Sperm quality after density gradient centrifugation with three commercially available media: A controlled trial

Reproductive Biology and Endocrinology

Volumn 12, Issue 1, 2014, Pages

  Malvezzi, Helena ^a   Sharma, Rakesh ^a   Agarwal, Ashok ^a   Abuzenadah, Adel M ^b   Abu Elmagd, Muhammad ^b,c  

^a LERNER RESEARCH INSTITUTE   (United States)

^b KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^c KACST Technology Innovation Center in Personalized Medicine King AbdulAziz University ^*  (Egypt)

Author keywords

Density gradient centrifugation; DNA fragmentation; Reproductive potential; Sperm motility; Sperm processing

Indexed keywords

DNA;

ADULT; ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DNA DAMAGE; DNA FRAGMENTATION; HUMAN; LEUKOCYTE; MALE; NORMAL HUMAN; SEMEN ANALYSIS; SPERM QUALITY; SPERMATOZOON DENSITY; SPERMATOZOON MOTILITY; CELL SEPARATION; CYTOLOGY; FLOW CYTOMETRY; METABOLISM; PHYSIOLOGY; PROCEDURES; SPERMATOZOON; SPERMATOZOON COUNT; YOUNG ADULT;

ADULT; CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; DNA DAMAGE; FLOW CYTOMETRY; HUMANS; MALE; SEMEN ANALYSIS; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA; YOUNG ADULT;

EID: 84924207283     PISSN: None     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/1477-7827-12-121     Document Type: Article

References (37)

1. Vanage GR, Gopalkrishnan K, Sheth AR: Effect of antibodies to human seminal plasma inhibin on spermatogenesis and sperm agglutination in adult male rats. Mol Reprod Dev 1990, 25:227-236. 10.1002/mrd.1080250304
2. Levay PF, Fourie FR, Meintjes J: The effect of seminal plasma on human sperm-zona pellucida binding. Hum Reprod 1995, 10:2590-2594.
3. Gil-Guzman E, Ollero M, Lopez MC, Sharma RK, Alvarez JG, Thomas AJ Jr, Agarwal A: Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001, 16:1922-1930. 10.1093/humrep/16.9.1922
4. Allamaneni SS, Agarwal A, Rama S, Ranganathan P, Sharma RK: Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatazoa. Asian J Androl 2005, 7:86-92. 10.1111/j.1745-7262.2005.00008.x
5. Beydola T, Sharma RK, Lee W, Agarwal A: Sperm preparation and selection techniques. In Male Infertility Practice. Edited by: Rizk B, Aziz N, Agarwal A. New Delhi: Jaypee Brothers Medical Publishers; 2013:244-251.
6. Boomsma CM, Heineman MJ, Cohlen BJ, Farquhar C: Semen preparation techniques for intrauterine insemination. Cochrane Database Syst Rev 2004, 3:CD004507.
7. Jayaraman V, Upadhya D, Narayan PK, Adiga SK: Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet 2012, 29:557-563. 10.1007/s10815-012-9742-x
8. Zini A, Nam RK, Mak V, Phang D, Jarvi K: Influence of initial semen quality on the integrity of human sperm DNA following semen processing. Fertil Steril 2000, 74:824-827. 10.1016/S0015-0282(00)01495-3
9. Zini A, Finelli A, Phang D, Jarvi K: Influence of semen processing technique on human sperm DNA integrity. Urology 2000, 56:1081-1084. 10.1016/S0090-4295(00)00770-6
10. Bungum M, Spanò M, Humaidan P, Eleuteri P, Rescia M, Giwercman A: Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART. Hum Reprod 2008, 23:4-10.
11. Brahem S, Mehdi M, Landolsi H, Mougou S, Elghezal H, Saad A: Semen parameters and sperm DNA fragmentation as causes of recurrent pregnancy loss. Urology 2011, 78:792-796. 10.1016/j.urology.2011.05.049
12. Brahem S, Mehdi M, Elghezal H, Saad A: Semen processing by density gradient centrifugation is useful in selecting sperm with higher double-strand DNA integrity. Andrologia 2011, 43:196-202. 10.1111/j.1439-0272.2010.01050.x
13. Enciso M, Iglesias M, Galán I, Sarasa J, Gosálvez A, Gosálvez J: The ability of sperm selection techniques to remove single- or double-strand DNA damage. Asian J Androl 2011, 13:764-768. 10.1038/aja.2011.46
14. Lopes S, Sun JG, Jurisicova A, Meriano J, Casper RF: Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril 1998, 69:528-532. 10.1016/S0015-0282(97)00536-0
15. Muratori M, Piomboni P, Baldi E, Filimberti E, Pecchioli P, Moretti E, Gambera L, Baccetti B, Biagiotti R, Forti G, Maggi M: Functional and ultrastructural features of DNA-fragmented human sperm. J Androl 2000, 21:903-912.
16. Sharma RK, Sabanegh E, Mahfouz R, Gupta S, Thiyagarajan A, Agarwal A: TUNEL as a test for sperm DNA damage in the evaluation of male infertility. Urology 2010, 76:1380-1386. 10.1016/j.urology.2010.04.036
17. Sharma RK, Agarwal A: Laboratory evaluation of sperm chromatin: TUNEL assay. In Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction. Edited by: Zini A, Agarwal A. New York: Springer Science + Business Media; 2011:201-215.
18. Mahfouz R, Sharma R, Thiyagarajan A, Kale V, Gupta S, Sabanegh E, Agarwal A: Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species. Fertil Steril 2010, 94:2141-2146. 10.1016/j.fertnstert.2009.12.030
19. Agarwal A, Varghese AC, Sharma RK: Markers of oxidative stress and sperm chromatin integrity. Methods Mol Biol 2009, 590:377-402. 10.1007/978-1-60327-378-7_24
20. Makker K, Agarwal A, Sharma R: Oxidative stress & male infertility. Indian J Med Res 2009, 129:357-367.
21. Abd-Elmoaty MA, Saleh R, Sharma R, Agarwal A: Increased levels of oxidants and reduced antioxidants in semen of infertile men with varicocele. Fertil Steril 2010, 94:1531-1534. 10.1016/j.fertnstert.2009.12.039
22. Benchaib M, Ajina M, Lornage J, Niveleau A, Durand P, Guérin JF: Quantitation by image analysis of global DNA methylation in human spermatozoa and its prognostic value in in vitro fertilization: a preliminary study. Fertil Steril 2003, 80:947-953. 10.1016/S0015-0282(03)01151-8
23. Virant-Klun I, Tomazevic T, Meden-Vrtovec H: Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J Assist Reprod Genet 2002, 19:319-328. 10.1023/A:1016006509036
24. Sun JG, Jurisicova A, Casper RF: Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod 1997, 56:602-607. 10.1095/biolreprod56.3.602
25. Larson KL, DeJonge CJ, Barnes AM, Jost LK, Evenson DP: Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod 2000, 15:1717-1722. 10.1093/humrep/15.8.1717
26. Lewis SE, Aitken RJ: DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 2005, 322:33-41. 10.1007/s00441-005-1097-5
27. Chiamchanya C, Kaewnoonual N, Visutakul P, Manochantr S, Chaiya J: Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters. Asian J Androl 2010, 12:271-277. 10.1038/aja.2009.60
28. World Health Organization: WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th edition. Geneva, Switzerland;
29. Sharma R, Masaki J, Agarwal A: Sperm DNA fragmentation analysis using the TUNEL assay. Methods Mol Biol 2013, 927:121-136. 10.1007/978-1-62703-038-0_12
30. Chi HJ, Kim JH, Ryu CS, Lee JY, Park JS, Chung DY, Choi SY, Kim MH, Chun EK, Roh SI: Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa. Hum Reprod 2008, 23:1023-1028. 10.1093/humrep/den060
31. Benchaib M, Lornage J, Mazoyer C, Lejeune H, Salle B, François Guerin J: Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril 2007, 87:93-100. 10.1016/j.fertnstert.2006.05.057
32. Li Z, Zhou Y, Liu R, Lin H, Liu W, Xiao W, Lin Q: Effects of semen processing on the generation of reactive oxygen species and mitochondrial membrane potential of human spermatozoa. Andrologia 2012, 44:157-163. 10.1111/j.1439-0272.2010.01123.x
33. Marchesi DE, Biederman H, Ferrara S, Hershlag A, Feng HL: The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel Toluidine Blue assay. Eur J Obstet Gynecol Reprod Biol 2010, 151:176-180. 10.1016/j.ejogrb.2010.05.003
34. Zhang XD, Chen MY, Gao Y, Han W, Liu DY, Huang GN: The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation. Hum Fertil (Camb) 2011, 14:187-191. 10.3109/14647273.2011.604817
35. Stevanato J, Bertolla RP, Barradas V, Spaine DM, Cedenho AP, Ortiz V: Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates. Fertil Steril 2008, 90:889-890. 10.1016/j.fertnstert.2007.01.059
36. Lewis SE, Simon L: Clinical implications of sperm DNA damage. Hum Fertil (Camb) 2010, 13:201-207. 10.3109/14647273.2010.528823
37. Jiang H, He RB, Wang CL, Zhu J: The relationship of sperm DNA fragmentation index with the outcomes of in-vitro fertilisation-embryo transfer and intracytoplasmic sperm injection. J Obstet Gynaecol 2011, 31:636-639. 10.3109/01443615.2011.590910