Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

Journal of Assisted Reproduction and Genetics

Volumn 29, Issue 6, 2012, Pages 557-563

  Jayaraman, Varshini ^a   Upadhya, Dinesh ^a   Narayan, Pratap Kumar ^a   Adiga, Satish Kumar ^a  

^a KASTURBA MEDICAL COLLEGE   (India)

Author keywords

Density gradient; Semen preparation; Sperm DNA damage; Sperm selection; Swim up

Indexed keywords

ARTICLE; CHROMATIN; CONTROLLED STUDY; DENSITY GRADIENT; DNA DAMAGE; HUMAN; INCIDENCE; MAJOR CLINICAL STUDY; MALE; NICK END LABELING; OLIGOSPERMIA; PREGNANCY; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON ABNORMALITY; STATISTICAL SIGNIFICANCE;

CENTRIFUGATION, DENSITY GRADIENT; DNA DAMAGE; HUMANS; IN SITU NICK-END LABELING; INFERTILITY, MALE; MALE; OLIGOSPERMIA; REFERENCE VALUES; SEMEN; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA;

EID: 84864942223     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-012-9742-x     Document Type: Article

References (33)

1. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;1:108.
2. Aitken RJ, Clarkson JS. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl. 1988;9:367-76
3. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril. 1992;57:409-16
4. Griveau JF, Le Lannou D. Effects of antioxidants on human sperm preparation techniques. Int J Androl. 1994;17:225-31
5. Aitken RJ, Buckingham DW, Brindle J, Gomez E, Baker HW, Irvine DS. Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma. Hum Reprod. 1995;10:2061-71
6. Zalata A, Hafez T, Comhaire F. Evaluation of the role of reactive oxygen species in male infertility. Hum Reprod. 1995;10:1444-51
7. Mortimer D. Sperm preparation techniques and iatrogenic failures of in vitro fertilization. Hum Reprod. 1991;6:173-6.
8. Oehninger S, Blackmore P, Mahony M, Hodgen G. Effects of hydrogen peroxide on human spermatozoa. J Assist Reprod Genet. 1995;12:41-7.
9. Le Lannou D, Blanchard Y. Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim-up procedure. J Reprod Fertil. 1988;84:551-6.
10. Ng FL, Liu DY, Baker HW. Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples. Hum Reprod. 1992;7:261-6.
11. Chen SU, Ho HN, Chen HF, Chao KH, Lin HR, Huang SC, et al. Comparison between a two-layer discontinuous Percoll gradient and swim-up for sperm preparation on normal and abnormal semen samples. J Assist Reprod Genet. 1995;12:698-70
12. Chen SU, Ho HN, Chen HF, Chao KH, Wu MY, Chen CD, et al. Combination of direct swim-up technique and discontinuous Percoll gradient centrifugation for sperm preparation of oligoasthenozoospermic samples. Arch Androl. 1996:37;103-9.
13. Prakash P, Leykin L, Chen Z, Toth T, Sayegh R, Schiff I, et al. Preparation by differential gradient centrifugation is better than swim-up in selecting sperm with normal morphology (strict criteria). Fertil Steril. 1998;69:722-6.
14. Sakkas D, Manicardi GC, Tomlinson M, Mandrioli M, Bizzaro D, Bianchi PG, et al. The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod. 2000;15:1112-6.
15. Zini A, Finelli A, Phang D, Jarvi K. Influence of semen processing technique on human sperm DNA integrity. Urology. 2000;56:1081-4.
16. Zini A, Nam RK, Mak V, Phang D, Jarvi K. Influence of initial semen quality on the integrity of human sperm DNA following semen processing. Fertil Steril. 2000;74:824-7.
17. Ahmad L, Jalali S, Shami SA, Akram Z. Sperm preparation: DNA damage by comet assay in normo-and teratozoospermics. Arch Androl. 2007;53:325-38
18. Hashimoto S, Goda S, Akamatsu Y, Yamanaka M, Morimoto Y. Effects of sperm preparation on sperm DNA fragmentation and morphology. RBM Online. 2008;16:S28.
19. RicciG, Perticarari S, Boscolo R, MonticoM, Guaschino S, PresaniG. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertil Steril. 2009;91:632-8.
20. Boomsma CM, Heineman MJ, Cohlen BJ, Farquhar C. Semen preparation techniques for intrauterine insemination. Cochrane Database Syst Rev. 2004;3:CD004507.
21. Boomsma CM, Heineman MJ, Cohlen BJ, Farquhar C. Semen preparation techniques for intrauterine insemination. Cochrane Database Syst Rev. 2007;4:CD004507.
22. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge: Cambridge University Press; 1999.
23. Spanò M, Toft G, Hagmar L, Eleuteri P, Rescia M, Rignell-Hydbom A, et al. Exposure to PCB and p, p'-DDE in European and Inuit populations: impact on human sperm chromatin Integrity. Hum Reprod. 2005;20:3488-99
24. Sakkas D, Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril. 2010;93:1027-36
25. Bungum M, Humaidan P, AxmonA, Spano M, Bungum L, Erempreiss J, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod. 2007;22:174-9.
26. Bungum M, Spanò M, Humaidan P, Eleuteri P, Rescia M, Giwercman A. Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART. Hum Reprod. 2008;23:4-10
27. Gosálvez J, Cortés-Gutiérrez EI, Nuñez R, Fernández JL, Caballero P, López-Fernández C, et al. A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors. Fertil Steril. 2009;92:1915-9.
28. Dalzell LH, McVicar CM, McClure N, Lutton D, Lewis SE. Effects of short and long incubations on DNA fragmentation of testicular sperm. Fertil Steril. 2004;82:1443-5.
29. Bormann C, Rocha A, Hassun P, Motta E, Serafini P, Smith G. The effect of sperm separation on sperm chromatin decondensation and motility at 0 and 24 hours of culture. Fertil Steril. 2008;90:S452-3.
30. Zini A, Mak V, Phang D, Jarvi K. Potential adverse effect of semen processing on human sperm deoxyribonucleic acid integrity. Fertil Steril. 1999;72:496-9.
31. Stevanato J, Bertolla RP, Barradas V, Spaine DM, Cedenho AP, Ortiz V. Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates. Fertil Steril. 2008;90:889-90
32. Twigg J, Irvine DS, Houston P, Fulton N, Michael L, Aitken RJ. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma. Mol Hum Reprod. 1998;4:439-45
33. Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, et al. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod. 2009;24:2409-16.