Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Della Cristina, Pietro ^a   Castagna, Monica ^a   Lombardi, Alessio ^b   Barison, Erika ^a   Tagliabue, Giovanni ^b   Ceriotti, Aldo ^b   Koutris, Ilias ^c   Di Leandro, Luana ^c   Giansanti, Francesco ^c   Vago, Riccardo ^c   Ippoliti, Rodolfo ^c   Flavell, Sopsamorn U ^d   Flavell, David J ^d   Colombatti, Marco ^a   Fabbrini, Serena ^b,e  

^a UNIVERSITY OF VERONA   (Italy)

^b CNR Consiglio Nazionale delle Ricerche   (Italy)

^c UNIVERSITY OF L'AQUILA   (Italy)

^d SOUTHAMPTON GENERAL HOSPITAL   (United Kingdom)

^e Istituto Nazionale di Genetica Molecolare   (Italy)

Author keywords

Anti CD22; Bacterial eukaryotic expression systems; Pseudomonas exotoxin A; Recombinant immunotoxins; Saporin

Indexed keywords

CD22 ANTIGEN; CYTOTOXIC AGENT; PSEUDOMONAS EXOTOXIN; PSEUDOMONAS EXOTOXIN A; SAPORIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; BACTERIAL TOXIN; EXOTOXIN; HYBRID PROTEIN; IMMUNOTOXIN; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; RIBOSOME INACTIVATING PROTEIN 1; TOXA PROTEIN, PSEUDOMONAS AERUGINOSA; VIRULENCE FACTOR;

ADENOSINE DIPHOSPHATE RIBOSYLATION; ANIMAL EXPERIMENT; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DAUDI CELL LINE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HUMAN; IC50; KOMAGATAELLA PASTORIS; MOUSE; NONHUMAN; PROTEIN SYNTHESIS INHIBITION; TARGET CELL; BURKITT LYMPHOMA; CELL LINE; CELL SURVIVAL; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECTS; GENETICS; IMMUNOLOGY; METABOLISM; PATHOLOGY; PICHIA; PROTEIN SYNTHESIS; TUMOR CELL LINE; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); PICHIA PASTORIS; PSEUDOMONAS EXOTOXIN;

ADP RIBOSE TRANSFERASES; BACTERIAL TOXINS; BLOTTING, WESTERN; BURKITT LYMPHOMA; CELL LINE; CELL LINE, TUMOR; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; EXOTOXINS; HUMANS; IMMUNOTOXINS; PICHIA; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; RIBOSOME INACTIVATING PROTEINS, TYPE 1; SIALIC ACID BINDING IG-LIKE LECTIN 2; SINGLE-CHAIN ANTIBODIES; VIRULENCE FACTORS;

EID: 84924121417     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0202-z     Document Type: Article

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