Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells

FASEB Journal

Volumn 14, Issue 2, 2000, Pages 391-398

  Fabbrini, M Serena ^a   Carpani, Daniela ^a   Soria, Marco R ^a   Ceriotti, Aldo ^a,b  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b CNR   (Italy)

Author keywords

Anti cancer therapy; Intracellular immunization; Ribosome inactivating proteins; Urokinase amino terminal fragment; Urokinase receptor

Indexed keywords

NEUTRALIZING ANTIBODY; RIBOSOME INACTIVATING PROTEIN; SAPORIN; UROKINASE RECEPTOR;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTINEOPLASTIC ACTIVITY; ARTICLE; CELL KILLING; CELL SURFACE; CELLULAR IMMUNITY; CHIMERA; CYTOSOL; CYTOTOXIC T LYMPHOCYTE; ENDOCYTOSIS; ENZYME BINDING; EUKARYOTE; GEL ELECTROPHORESIS; IMMUNIZATION; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SYNTHESIS; TARGET CELL;

ANIMALIA; EUKARYOTA;

EID: 0033950004     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.14.2.391     Document Type: Article

References (47)

1. Fitzgerald, D. (1996) Why toxins! Semin. Cancer Biol. 7, 87-95
2. Pai, L., H., Wittes, R., Setser, A., Willingham, M. C., and Pastan, I. (1996) Treatment of advanced solid tumors with immunotoxin LMB-1: an antibody linked to Pseudomonas exotoxin. Nature Med. 2, 350-353
3. Laske, D. W., Youle, R. J., and Oldfield, E., H. (1997) Tumor regression with regional distribution of the targeted toxin TF-CRM107 in patients with malignant brain tumors. Nature Med. 3, 1362-1368
4. Mantyh, P. W., Rogers, S. D., Honore, P., Allen, B. J., Ghilardi, J. R., Li, J., Daughters, R. S., Lappi, D. A., Wiley, R. G., and Simone, D. A. (1997) Inhibition of hyperalgesia by ablation of lamina I spinal neurons expressing the substance P receptor. Science 278, 275-279
5. Flavell, D. J. (1998) Saporin immunotoxins. Curr. Top. Microbiol. Immunol. 234, 57-61
6. Pastan, I. (1997) Targeted therapy of cancer with recombinant immunotoxins. Biochim. Biophys. Acta 1333, C1-C6
7. Leitzgen, K., and Haas, I. G. (1998) Protein maturation in the endoplasmic reticulum. Chemtracts-Biochem. Mol. Biol. 11, 423-445
8. Fabbrini, M. S., Carpani, D., Bello-Rivero, I., and Soria, M. R. (1997) The amino-terminal fragment of human urokinase directs a recombinant chimeric toxin to target cells: internalization is toxin mediated. FASEB J. 11, 1169-1176
9. Endo, Y., Tsurugi, K., and Lambert, J. M. (1988) The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins. Biochem. Biophys. Res. Commun. 150, 1032-1036
10. Andreasen, P. A., Kjøller, L., Christensen, L., and Duffy, M. J. (1997) The urokinase-type plasminogen activator system in cancer metastasis: a review. Int. J. Cancer 72, 1-22
11. Blasi, F., and Stoppelli, M. P. (1998) Proteases and cancer invasion: from belief to certainty. Biochim. Biophys. Acta 1423, R35-R44
12. Kim, J., Yu, K., and Ossowski, L. (1998) Requirement for specific proteases in cancer cell intravasation as revealed by a novel semiquantitative PCR-based assay. Cell 94, 353-362
13. Cavallaro, U., Nykjær, A., Nielsen, M., and Soria, M. R. (1995) α2-Macroglobulin receptor mediates binding and cytotoxicity of plant ribosome-inactivating proteins. Eur. J. Biochem. 232, 165-171
14. Fabbrini, M. S., Rappocciolo, E., Carpani, D., Solinas, M., Valsasina, B., Breme, U., Cavallaro, U., Nykjaer, A., Rovida, E., Legname, G., and Soria, M. R. (1997) Characterization of a saporin isoform with lower ribosome-inhibiting activity. Biochem. J. 322, 719-727
15. Hur, Y., Hwang, D.-J., Zoubenko, O., Coetzer, C., Uckun, F. M., and Tumer, N. E. (1995) Isolation and characterization of pokeweed antiviral protein mutations in Saccharomices cerevisiae: identification of residues important for toxicity. Proc. Natl. Acad. Sci. USA 92, 8448-8452
16. Frigerio, L., Vitale, A., Lord, J. M., Ceriotti, A., and Roberts, L. M. (1998) Free ricin A chain, proricin and native toxin have different cellular fates when expressed in tobacco protoplasts. J. Biol. Chem. 273, 14194-14199
17. Brandazza, A., Lee, E., Ferrera, M., Tillman, U., Sarmientos, P., and Westphal, H. (1991) Use of the urokinase-type plasminogen activator gene as a general tool to monitor expression in transgenic animals: study of the tissue-specificity of the murine whey acidic protein expression signals. J. Biotechnol. 20, 201-212
18. Krieg, P. A., and Melton, D. A. (1984) Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 12, 7057-7070
19. Ceriotti, A., Pedrazzini, E., Fabbrini, M. S., Zoppè, M., Bollini, R., and Vitale, A. (1991) Expression of wild-type and mutated vacuolar storage protein phaseolin in Xenopus oocytes reveals relationships between assembly and intracellular transport. Eur. J. Biochem. 202, 959-968
20. Santoro, C., Marone, M., Ferrone, M., Costanzo, M., Colombo, M., Minganti, C., Cortese, R., and Silengo, L. (1986) Cloning of the gene coding for human L apoferritin. Nucleic Acids Res. 14, 2863-2876
21. Ceriotti, A., Pedrazzini, E., de Silvetris, M., and Vitale, A. (1995) (Galbraith, D. W., Bourque, D. P., and Bohnert, H. J., eds) In Methods in Cell Biology, Vol. 50B, chap. 21, pp. 295-308, Academic Press, San Diego
22. Sambrook, T., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual (Nolan, C., ed) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
23. Ceriotti, A., and Colman, A. (1995) mRNA Translation in Xenopus Oocytes (Tymms, M. J., ed) In Methods in Molecular Biology, Vol. 37, pp. 151-178, Humana Press, Totowa, New Jersey
24. Fabbrini, M. S., Vitale, A., Pedrazzini, E., Nitti, G., Zamai, M., Tamburin, M., Caiolfa, V. R., Patrono, C., and Benatti, L. (1993) In vivo expression of mutant preproendothelins: hierarchy of processing events but no strict requirement of Trp-Val at the processing site. Proc. Natl. Acad. Sci. USA 90, 3923-3927
25. Corti, A., Sarubbi, E., Nolli, M. L., Zanni, A., Galimberti, M., Parenti, F., and Cassani, G. (1989) Epitope mapping of the anti-urokinase monoclonal antibody 5B4 by isolated domains of urokinase. Thromb. Haemost. 62, 934-939
26. Galili, G., Altschuler, Y., and Ceriotti, A. (1995) (Galbraith, D. P. B., Bourque, D. P., and Bohnert, H. J., eds) In Methods in Cell Biology, Vol. 50B, chap. 36, pp. 497-517, Academic Press, San Diego
27. Laemmli, U. K., and Favre, M. (1973) Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol. 80, 575-599
28. Bonner, W. M., and Laskey, R. A. (1974) A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46, 83-88
29. Roldan, A. L., Cubellis, M. V., Masucci, M. T., Behrendt, N., Lund, L. R., Dano, K., Appella, E., and Blasi, F. (1990) Cloning and expression of the receptor for human urokinase plasminogen activator; a central molecule in cell surface, plasmin dependent proteolysis. EMBO J. 9, 467-474
30. Olson, D., Pollanen, J., Hoyer-Hansen, G., Ronne, E., Sakaguchi, K., Wun, T.-C., Appella, E., Dano, K., and Blasi, F. (1992) Internalization of the urokinase-plasminogen activator inhibitor type-1 complex is mediated by the urokinase Receptor. J. Biol. Chem. 267, 9129-9133
31. May, J. M., Hartley, M. R., Roberts, L. M., Krieg, P. A., Osborn, R. W., and Lord, J. M. (1989) Ribosome inactivation by ricin A chain: a sensitive method to asses the activity of wild-type and mutant polypeptides. EMBO J. 8, 301-308
32. Saxena, S. K., O'Brien, A. D., and Ackerman, E. J. (1989) Shiga toxin, Shiga-like toxin II variant, and ricin are all single-site RNA N-glycosidases of 28S RNA when microinjected into Xenopus oocytes. J. Biol. Chem. 264, 596-601
33. Richter, J. D., and Smith, L. D. (1981) Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane bound polysomes. Cell 27, 183-191
34. Vassalli, J. D., Dayer, J. M., Wohlwend, A., and Belin, D. (1984) Concomitant secretion of prourokinase and of plasminogen activator-specific inhibitor by cultured human monocytes-macrophages. J. Exp. Med. 159, 1653-1668
35. Benatti, L., Saccardo, M. B., Dani, M., Nitti, G., Sassano, M., Lorenzetti, R., Lappi, D. A., and Soria, M. (1989) Nucleotide sequence of cDNA coding for saporin-6, a type-1 ribosome-inactivating protein from Saponaria officinalis. Eur. J. Biochem. 183, 465-470
36. Carzaniga, R., Sinclair, L., Fordhamskelton, A. P., Harris, N., and Croy, R. R. D. (1994) Cellular and subcellular distribution of saporins, type-1 ribosome-inactivating proteins, in soapwort (Saponaria officinalis L.). Planta 194, 461-470
37. Mollenhauer, H. H., Morre, D. J., and Rowe, L. D. (1990) Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity. Biochim. Biophys. Acta 7, 225-246
38. Cavallaro, U., del Vecchio, A., Lappi, D. A., and Soria, M. R. (1993) A conjugate between human urokinase and saporin, a type I ribosome-inactivating protein is selectively cytotoxic in urokinase receptor-expressing cells. J. Biol. Chem. 268, 23186-23190
39. Nykjaer, A., Kjøller, L., Cohen, R. L., Lawrence, D. A., Garni-Wagner, B. A., Todd, R. F., van Zonneveld, A. J., Gliemann, J., and Andreasen, P. A. (1994) Regions involved in binding of uPA inhibitor complex and pro-uPA to LRP. Evidence that the uPAR protects pro-uPA against binding to the endocytic receptor. J. Biol. Chem. 269, 25668-25676
40. Chen, S.-Y., Yang, A.-G., Chen, J.-D., Kute, T., King, C. R., Collier, J., Cong, Y., Yao, C., and Huang, X. F. (1997) Potent antitumor activity of a new class of tumor-specific killer cells. Nature (London) 385, 78-80
41. Yang, A. G., and Chen, S.-Y. (1997) A new class of antigen-specific killer cells. Nature Biotechnol. 15, 46-51
42. Williams, M. D., Rostovtsev, A., Narla, R. K., and Uckun, F. M. (1998) Production of recombinant DTc1GMCSF fusion toxin in a baculovirus expression vector system for biotherapy of GMCSF-receptor positive hematologic malignancies. Prot. Express. Purif. 13, 210-221
43. Pappenheimer, A. M. (1977) Diphtheria toxin. Annu. Rev. Biochem. 46, 69-94
44. Rapak, A., Pal, O. F., and Olsnes, S. (1997) Retrograde transport of mutant ricin to the endoplasmic reticulum with subsequent translocation to cytosol. Proc. Natl. Acad. Sci. USA 94, 3783-3788
45. Lord, J., and Roberts, L. (1998) Toxin entry - retrograde transport through the secretory pathway. J. Cell Biol. 140, 733-736
46. Tavladoraki, P., Benvenuto, E., Trinca, S., De Martinis, D., Cattaneo, A., and Galeffi, P. (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature (London) 366, 469-472
47. Biocca, S., Pierandrei-Amaldi, P., Campioni, N., and Cattaneo, A. (1994) Intracellular immunization with cytosolic recombinant antibodies. Biotechnology 12, 396-399