Engineering a switchable toxin: The potential use of PDZ domains in the expression, targeting and activation of modified saporin variants

Protein Engineering, Design and Selection

Volumn 23, Issue 2, 2010, Pages 61-68

  Giansanti, Francesco ^a   Di Leandro, Luana ^a   Koutris, Ilias ^a   Pitari, Giuseppina ^a   Fabbrini, Maria Serena ^b   Lombardi, Alessio ^b   Flavell, David J ^c   Flavell, Sopsamorn U ^c   Gianni, Stefano ^d   Ippoliti, Rodolfo ^a  

^a UNIVERSITY OF L'AQUILA   (Italy)

^b CNR Consiglio Nazionale delle Ricerche   (Italy)

^c UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

PDZ domain; Recombinant expression; Ribosome inactivating protein; Saporin

Indexed keywords

CELLULAR DOMAIN; CO-EXPRESSION; CRITICAL PROBLEMS; HETEROLOGOUS SYSTEMS; HUMAN CANCER CELLS; NEW SYSTEM; PDZ DOMAINS; RECOMBINANT EXPRESSION; RIBOSOME-INACTIVATING PROTEIN; SAPORIN; STABILIZATION EFFECTS; SWITCHABLE; TOXIC PROTEINS;

BIOCHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; MAMMALS; TOXIC MATERIALS;

PROTEINS;

RIBOSOME INACTIVATING PROTEIN; SAPORIN; TOXIN;

ARTICLE; BACTERIAL CELL; BIOSYNTHESIS; CANCER CELL; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MAMMAL CELL; MOLECULAR STABILITY; NONHUMAN; PDZ DOMAIN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; PROTEIN VARIANT; RIBOSOME;

ANIMALS; CELL LINE; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; PDZ DOMAINS; PROTEIN ENGINEERING; PROTEIN SYNTHESIS INHIBITORS; RECOMBINANT PROTEINS; RIBOSOME INACTIVATING PROTEINS, TYPE 1; TRANSFECTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; MURINAE;

EID: 75649122575     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp070     Document Type: Article

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