Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains

Bioscience, Biotechnology and Biochemistry

Volumn 78, Issue 4, 2014, Pages 624-634

  Itoh, Takafumi ^a   Sugimoto, Ikumi ^a   Hibi, Takao ^a   Suzuki, Fumiko ^a   Matsuo, Koichi ^b   Fujii, Yutaka ^c   Taketo, Akira ^d   Kimoto, Hisashi Kimoto ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c UNIVERSITY OF FUKUI   (Japan)

^d FUKUI UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

Cell surface expressed chitinase; Chitin; Glycoside hydrolase family 18; Multi modular protein; Paenibacillus sp. FPU 7

Indexed keywords

CHITIN; DICHROISM; ENZYME ACTIVITY; ESCHERICHIA COLI; HYDROLASES; PROTEINS; PURIFICATION; SUGARS;

BIOCHEMICAL PROPERTIES; CELL SURFACE MOLECULES; CHITINASES; GLYCOSIDE HYDROLASES; MULTI-MODULAR; PAENIBACILLUS SP; SITE DIRECTED MUTAGENESIS; VACUUM ULTRAVIOLET CIRCULAR DICHROISM;

CELL MEMBRANES;

ESCHERICHIA COLI; PAENIBACILLUS; PAENIBACILLUS SP.;

CHITIN; CHITINASE;

AMINO ACID SEQUENCE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; MUTATION; PAENIBACILLUS;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CHITIN; CHITINASE; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PAENIBACILLUS; SUBSTRATE SPECIFICITY;

EID: 84924069481     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1080/09168451.2014.891935     Document Type: Article

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