Crystal structures of Bacillus cereus NCTU2 chitinase complexes with chitooligomers reveal novel substrate binding for catalysis: A chitinase without chitin binding and insertion domains

Journal of Biological Chemistry

Volumn 285, Issue 41, 2010, Pages 31603-31615

  Hsieh, Yin Cheng ^a,c   Wu, Yue Jin ^b   Chiang, Tzu Ying ^a,c   Kuo, Chueh Yuan ^a,c   Shrestha, Keshab Lal ^b   Chao, Cheng Fu ^b   Huang, Yen Chieh ^a   Chuankhayan, Phimonphan ^a   Wu, Wen Guey ^c   Li, Yaw Kuen ^b   Chen, Chun Jung ^a,c,d  

^a NATIONAL SYNCHROTRON RADIATION RESEARCH CENTER   (Taiwan)

^b NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

^c NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^d NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS CEREUS; BACTERIOLOGY; CATALYSIS; CHITIN; CONFORMATIONS; CRYSTAL STRUCTURE; HYDROLYSIS; SUGARS;

ACTIVE SITE; BINDING CLEFTS; CATALYTIC DOMAINS; CATALYTIC ROLE; CHITIN BINDING; CHITINASES; CHITOOLIGOMERS; CONFORMATIONAL CHANGE; GLYCOSIDE HYDROLASES; GLYCOSIDIC BOND; INTERMEDIATE STATE; KINETICS ANALYSIS; LINEAR POLYMERS; MULTIPLE CONFORMATIONS; N-ACETYL-D-GLUCOSAMINE; NUTRIENT SOURCES; PRODUCT RELEASE; SUBSTRATE BINDING; WILD TYPES; ZINC IONS;

SUBSTRATES;

ASPARTIC ACID; CHITIN; CHITINASE; CHITOBIOSE; CYCLO(HISTIDYLPROLINE); GLUTAMINE; GLYCINE; GLYCOSIDASE; ISOLEUCINE; N ACETYLGLUCOSAMINE; OLIGOMER; THREONINE; TYROSINE; VALINE; WATER; ZINC ION; BACTERIAL PROTEIN; DISACCHARIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS CEREUS; CHEMICAL BOND; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN INTERACTION; WILD TYPE; BINDING SITE; CATALYSIS; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MISSENSE MUTATION; PROTEIN SECONDARY STRUCTURE; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

BACILLUS CEREUS;

AMINO ACID SUBSTITUTION; BACILLUS CEREUS; BACTERIAL PROTEINS; BINDING SITES; CATALYSIS; CHITINASE; CRYSTALLOGRAPHY, X-RAY; DISACCHARIDES; KINETICS; MUTATION, MISSENSE; PROTEIN STRUCTURE, SECONDARY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 77957814635     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.149310     Document Type: Article

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