Engineering cellular redox balance in Saccharomyces cerevisiae for improved production of L-lactic acid

Biotechnology and Bioengineering

Volumn 112, Issue 4, 2015, Pages 751-758

  Lee, Ju Young ^a   Kang, Chang Duk ^a   Lee, Seung Hyun ^a   Park, Young Kyoung ^a   Cho, Kwang Myung ^a  

^a Samsung Advanced Institute of Technology (SAIT)   (South Korea)

Author keywords

L lactic acid; Redox balace engineering; S. cerevisiae

Indexed keywords

BIODEGRADABLE POLYMERS; ENCODING (SYMBOLS); GENE ENCODING; GENES; INDUSTRIAL CHEMICALS; METABOLIC ENGINEERING; METABOLISM; YEAST;

INDUSTRIAL BIOTECHNOLOGY; INDUSTRIAL PRODUCTION; L -LACTIC ACIDS; LACTIC ACID PRODUCTION; MICROBIAL CELL FACTORIES; NADH DEHYDROGENASE; PYRUVATE DECARBOXYLASE; S.CEREVISIAE;

LACTIC ACID;

LACTIC ACID; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; FED BATCH REACTOR; FUNGAL GENE; FUNGAL STRAIN; GLYCEROL 3 PHOSPHATE DEHYDROGENASE GENE; L LACTATE CYTOCHROME C OXIDOREDUCTASE GENE; LACTATE DEHYDROGENASE GENE; NONHUMAN; OXIDATION REDUCTION STATE; PH MEASUREMENT; PYRUVATE DECARBOXYLASE 1 GENE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE 1 GENE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE 2 GENE; SACCHAROMYCES CEREVISIAE; BATCH CELL CULTURE; BIOREACTOR; GENE DELETION; GENE EXPRESSION; GENETICS; METABOLIC ENGINEERING; METABOLISM; OXIDATION REDUCTION REACTION; PH;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; GENE DELETION; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; LACTIC ACID; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84924040473     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25488     Document Type: Article

References (47)

1. Adachi E, Torigoe M, Sugiyama M, Nikawa JI, Shimizu K. 1998. Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value. J Ferment Bioeng 86:284-289.
2. Adams BG. 1972. Induction of galactokinase in Saccharomyces cerevisiae: Kinetics of induction and glucose effects. J Bacteriol 111:308-313.
3. Alani E, Cao L, Kleckner N. 1987. A method for gene disruption that allows for repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541-545.
4. Albers E, Larsson C, Liden G, Niklasson C, Gustafsson L. 1996. Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation. Appl Environ Microbiol 62:3187-3195.
5. Aldiguier AS, Alfenore S, Cameleyre X, Goma G, Uribelarrea JL, Guillouet SE, Molina-Jouve C. 2004. Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behaviour in ethanol bio-fuel production. Bioprocess Biosyst Eng 26:217-222.
6. +-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16:2179-2187.
7. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. 1989. Current protocols in molecular biology. New York: John Wiley and Sons.
8. Baker SM, Johnston SA, Hopper JE, Jaehning JA. 1987. Transcription of multiple copies of the yeast GAL7 gene is limited by specific factors in addition to GALA. Mol Gen Genet 208:127-134.
9. Benninga HA. 1990. A History of Lactic Acid Making. Dordrecht: Kluwer Academic Publishers.
10. +) in acquired osmotolerance of Saccharomyces cerevisiae. J Bacteriol 171:1087-1092.
11. Bro C, Regenberg B, Nielsen J. 2004. Genome-wide transcriptional response of a Saccharomyces cerevisiae strain with an altered redox metabolism. Biotechnol Bioeng 85:269-276.
12. Colombié S, Dequin S, Sablayrolle JM. 2003. Control of lactate production by Saccharomyces cerevisiae expressing a bacterial LDH gene. Enzyme Microb Technol 70:38-46.
13. Datta R, Tsai SP, Bonsignore P, Moon SH, Frank JR. 1995. Technological and economic potential of poly(lactic acid) and lactic acid derivatives. FEMS Microbiol Rev 16:221-231.
14. Dequin S, Barre P. 1994. Mixed lactic acid-alcoholic fermentation by Saccharomyces cerevisiae expressing the Lactobacillus casei L(+)-LDH. Biotechnology 12:173-177.
15. Flikweert MT, de Swaaf M, van Dijken JP, Pronk JT. 1999. Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae. FEMS Microbiol Lett 174:73-79.
16. Gatignol A, Dassain M, Tiraby GC. 1990. Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance. Gene 91:35-41.
17. Geertman JM, van Dijken JP, Pronk JT. 2006. Engineering NADH metabolism in Saccharomyces cerevisiae: Formate as an electron donor for glycerol production by anaerobic, glucose-limited chemostat cultures. FEMS Yeast Res 6:1193-1203.
18. 2O-forming NADH oxidase and impact on redox metabolism. Metab Eng 8:303-314.
19. Hohmann S. 2002. Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol Rev 66:300-372.
20. Hou J, Lages NF, Oldiges M, Vemuri GN. 2009. Metabolic impact of redox cofactor perturbations in Saccharomyces cerevisiae. Metab Eng 11:253-261.
21. Ilmén M, Koivuranta K, Ruohonen L, Rajgarhia V, Suominen P, Penttilä M. 2013. Production of l-lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases. Microb Cell Fact 12:53.
22. Ishida N, Saitoh S, Tokuhiro K, Nagamori E, Matsuyama T, Kitamoto K, Takahashi H. 2005. Efficient production of L-Lactic acid by metabolically engineered Saccharomyces cerevisiae with a genome-integrated L-lactate dehydrogenase gene. Appl Environ Microbiol 71:1964-1970.
23. Ishida N, Saitoh S, Ohnishi T, Tokuhiro K, Nagamori E, Kitamoto K, Takahashi H. 2006a. Metabolic engineering of Saccharomyces cerevisiae for efficient production of pure L-(+)-lactic acid. Appl Biochem Biotechnol 131:795-807.
24. Ishida N, Saitoh S, Ohnishi T, Tokuhiro K, Nagamori E, Kitamoto K, Takahashi H. 2006b. The effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production. Biosci Biotechnol Biochem 70:1148-1153.
25. Kim Y, Ingram LO, Shanmugam KT. 2007. Construction of an Escherichia coli K-12 mutant for homoethanologenic fermentation of glucose or xylose without foreign genes. Appl Environ Microbiol 73:1766-1771.
26. Kim Y, Ingram LO, Shanmugam KT. 2008. Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12. J Bacteriol 190:3851-3858.
27. Laughon A, Gesteland RF. 1982. Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast. Proc Natl Acad Sci USA 79:6827-6831.
28. Luttik MA, Overkamp KM, Kötter P, de Vries S, van Dijken JP, Pronk JT. 1998. The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. J Biol Chem 273:24529-24534.
29. Luyten K, Albertyn J, Skibbe WF, Prior BA, Ramos J, Thevelein JM, Hohmann S. 1995. Fps1, a yeast member of the MIP family of channel proteins, is a facilitator for glycerol uptake and efflux and is inactive under osmotic stress. EMBO J 14:1360-1371.
30. Marres CA, de Vries S, Grivell LA. 1991. Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: Ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem 195:857-862.
31. Mumberg D, Muller R, Funk M. 1995. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156:119-122.
32. Ogden JE, Stanway C, Kim S, Mellor J, Kingsman AJ, Kingsman SM. 1986. Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activating sequence but does not require TATA sequences. Mol Cell Biol 6:4335-4343.
33. Oura E. 1977. Reaction-products of yeast fermentations. Process Biochem 12:19-21.
34. Pei J, Zhou Q, Jing Q, Li L, Dai C, Li H, Wiegel J, Shao W. 2011. The mechanism for regulating ethanol fermentation by redox levels in Thermoanaerobacter ethanolicus. Metab Eng 13:186-193.
35. Porro D, Brambilla L, Ranzi BM, Martegani E, Alberghina L. 1995. Development of metabolically engineered Saccharomyces cerevisiae cells for the production of lactic acid. Biotechnol Prog 11:294-298.
36. Porro D, Bianchi MM, Brambilla L, Menghini R, Bolzani D, Carrera V, Lievense J, Liu CL, Ranzi BM, Frontali L, Alberghina L. 1999. Replacement of a metabolic pathway for large-scale production of lactic acid from engineered yeasts. Appl Environ Microbiol 65:4211-4215.
37. Partow S, Siewers V, Bjørn S, Nielsen J, Maury J. 2010. Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27:955-964.
38. Remize F, Cambon B, Barnavon L, Dequin S. 2003. Glycerol formation during wine fermentation is mainly linked to Gpd1p and is only partially controlled by the HOG pathway. Yeast 20:1243-1253.
39. Rigoulet M, Aguilaniu H, Avéret N, Bunoust O, Camougrand N, Grandier-Vazeille X, Larsson C, Pahlman IL, Manon S, Gustafsson L. 2004. Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae. Mol Cell Biochem 73:256-257.
40. Saitoh S, Ishida N, Onishi T, Tokuhiro K, Nagamori E, Kitamoto K, Takahashi H. 2005. Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity. Appl Environ Microbiol 71:2789-2792.
41. Shen CR, Lan EI, Dekishima Y, Baez A, Cho KM, Liao JC. 2011. Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli. Appl Environ Microbiol 77:2905-2915.
42. Skory CD. 2003. Lactic acid production by Saccharomyces cerevisiae expressing a Rhizopus oryzae lactate dehydrogenase gene. J Ind Microbiol Biotechnol 30:22-27.
43. van Dijken J, Scheffers WA. 1986. Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol Lett 32:199-224.
44. van Maris AJ, Winkler AA, Porro D, van Dijken JP, Pronk JT. 2004. Homofermentative lactate production cannot sustain anaerobic growth of engineered Saccharomyces cerevisiae: Possible consequence of energy-dependent lactate export. Appl Environ Microbiol 70:2898-2905.
45. Vemuri GN, Eiteman MA, McEwen JE, Olsson L, Nielsen J. 2007. Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 104:2402-2407.
46. von Jagow G, Klingenberg M. 1970. Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis. Eur J Biochem 12:583-592.
47. Wang Y, Tashiro Y, Sonomoto K. 2014. Fermentative production of lactic acid from renewable materials: Recent achievements, prospects, and limits. J Biosci Bioeng 14:002199.