Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae

Journal of Visualized Experiments

Volumn , Issue 96, 2015, Pages

  Watts, Sheldon G ^a   Crowder, Justin J ^a   Coffey, Samuel Z ^a,b   Rubenstein, Eric M ^a  

^a BALL STATE UNIVERSITY   (United States)

^b Cincinnati Children's Hospital   (United States)

Author keywords

Budding yeast; Endoplasmic reticulum associated degradation; Growth assay; Issue 96; Molecular biology; Mutants; Protein degradation; Protein extracts; Saccharomyces cerevisiae; Ubiquitin proteasome system; Western blotting; Yeast genetics

Indexed keywords

SACCHAROMYCES CEREVISIAE PROTEIN;

GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MUTATION; PHYSIOLOGY; PROTEIN DEGRADATION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING;

BLOTTING, WESTERN; MUTATION; PROTEOLYSIS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84923614495     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52428     Document Type: Article

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