When less becomes more: Optimization of protein expression in HEK293-EBNA1 cells using plasmid titration - A case study for NLRs

Protein Expression and Purification

Volumn 99, Issue , 2014, Pages 27-34

  Halff, Els F ^a   Versteeg, Marian ^a   Brondijk, T Harma C ^a   Huizinga, Eric G ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

HEK293E cells; Nod like receptors; Protein aggregation; Recombinant protein expression; Transient transfection

Indexed keywords

CALCIUM BINDING PROTEIN; CASPASE RECRUITMENT DOMAIN SIGNALING PROTEIN; CELL RECEPTOR; EBV-ENCODED NUCLEAR ANTIGEN 1; EPSTEIN BARR VIRUS ANTIGEN; GREEN FLUORESCENT PROTEIN; NLRC4 PROTEIN, HUMAN; POLYETHYLENEIMINE; PROTEIN AGGREGATE; RECOMBINANT PROTEIN;

CELL DEATH; CHEMISTRY; DRUG EFFECTS; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PLASMID; PROCEDURES; PROTEIN FOLDING; SOLUBILITY;

CALCIUM-BINDING PROTEINS; CARD SIGNALING ADAPTOR PROTEINS; CELL DEATH; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; PLASMIDS; POLYETHYLENEIMINE; PROTEIN AGGREGATES; PROTEIN FOLDING; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT PROTEINS; SOLUBILITY; TRANSFECTION;

EID: 84897978175     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.03.010     Document Type: Article

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