Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13

Journal of Bacteriology

Volumn 179, Issue 11, 1997, Pages 3801-3803

  Kasberg, Thomas ^a   Seibert, Volker ^b   Schlömann, Michael ^b   Reineke, Walter ^a  

^a UNIVERSITY OF WUPPERTAL   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; MALEYLACETATE REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BURKHOLDERIA CEPACIA; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA CEPACIA; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0030983575     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.11.3801-3803.1997     Document Type: Article

References (20)

1. Chatterjee, D. K., S. T. Kellogg, S. Hamada, and A. M. Chakrabarty. 1981. Plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway. J. Bacteriol. 146:639-646.
2. Daubaras, D. L., C. D. Hershberger, K. Kitano, and A. M. Chakrabarty. 1995. Sequence analysis of a gene cluster involved in metabolism of 2,4,5-trichlorophenoxyacetic acid by Burkholderia cepacia AC1100. Appl. Environ. Microbiol. 61:1279-1289.
3. Daubaras, D. L., K. Saido, and A. M. Chakrabarty. 1996. Purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by Burkholderia cepacia AC1100. Appl. Environ. Microbiol. 62:4276-4279.
4. Don, R. H., A. J. Weightman, H.-J. Knackmuss, and K. N. Timmis. 1985. Transposon mutagenesis and cloning analysis of the pathways for the degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4). J. Bacteriol. 161:85-90.
5. Dorn, E., M. Hellwig, W. Reineke, and H.-J. Knackmuss. 1974. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch. Microbiol. 99:61-70.
6. Frantz, B., and A. M. Chakrabarty. 1987. Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation. Proc. Natl. Acad. Sci. USA 84:4460-4464.
7. Frantz, B., K.-L. Ngai, D. K. Chatterjee, L. N. Ornston, and A. M. Chakrabarty. 1987. Nucleotide sequence and expression of clcD, a plasmid-born dienelactone hydrolase gene from Pseudomonas sp. strain B13. J. Bacteriol. 169:704-709.
8. Gaal, A. B., and H. Y. Neujahr. 1980. Maleylacetate reductase from Trichosporon cutaneum. Biochem. J. 185:783-786.
9. Kasberg, T., D. L. Daubaras, A. M. Chakrabarty, D. Kinzelt, and W. Reineke. 1995. Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway. J. Bacteriol. 177:3885-3889.
10. Kasberg, T. Unpublished results.
11. Kaschabek, S. R., and W. Reineke. 1992. Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds. Arch. Microbiol. 158:412-417.
12. Kaschabek, S. R., and W. Reineke. 1993. Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13. J. Bacteriol. 175:6075-6081.
13. Kaschabek, S. R., and W. Reineke. 1995. Maleylacetate reductase of Pseudomonas sp. strain B13: specificity of substrate conversion and halide elimination. J. Bacteriol. 177:320-325.
14. Müller, D., M. Schlömann, and W. Reineke. 1996. Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties. J. Bacteriol. 178:298-300.
15. Perkins, E. J., M. V. Gordon, O. Caceres, and P. F. Lurquin. 1990. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J. Bacteriol. 172:2351-2359.
16. Schell, U., V. Seibert, M. Vollmer, and M. Schlömann. 1994. TfdF - a second, plasmid-encoded maleylacetate reductase of Alcaligenes eutrophus JMP134 (pJP4). Bioengineering Special issue, abstr. P413, p. 83.
17. Schell, U. Unpublished results.
18. Seibert, V., K. Stadler-Fritzsche, and M. Schlömann. 1993. Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134 (pJP4). J. Bacteriol. 175:6745-6754.
19. van der Meer, J. R., W. M. de Vos, S. Harayama, and A. J. B. Zehnder. 1992. Molecular mechanisms of genetic adaptation to xenobiotic compounds. Microbiol. Rev. 56:677-694.
20. van der Meer, J. R., R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos. 1991. Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of the catechol 1,2-dioxygenases for chlorinated substrates. J. Bacteriol. 173: 2425-2434.