Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids

Metabolic Engineering

Volumn 28, Issue , 2015, Pages 202-212

  Clomburg, James M ^a   Blankschien, Matthew D ^a   Vick, Jacob E ^a   Chou, Alexander ^a   Kim, Seohyoung ^a   Gonzalez, Ramon ^a  

^a Rice University   (United States)

Author keywords

Fuels and chemicals; Metabolic engineering; Synthetic biology; oxidation reversal; oxidation

Indexed keywords

BACTERIA; CARBON; CARBOXYLIC ACIDS; CHAINS; ENZYMES; GLYCEROL; METABOLIC ENGINEERING; SYNTHETIC FUELS;

ALDEHYDE DEHYDROGENASE; DICARBOXYLIC ACID; EXPRESSION SYSTEM; FUNCTIONALIZATIONS; INDEPENDENT CONTROL; INTEGRATED ENGINEERING; PSEUDOMONAS PUTIDA; SYNTHETIC BIOLOGY;

OXIDATION;

10 HYDROXYDECANOIC ACID; 6 HYDROXYHEXANOIC ACID; 8 HYDROXYOCTANOIC ACID; ACETYL COENZYME A ACETYLTRANSFERASE; ADIPIC ACID; DICARBOXYLIC ACID; GLYCEROL; HEXANOIC ACID DERIVATIVE; HYDROXYACID; OCTANOIC ACID DERIVATIVE; SEBACIC ACID; THIOL ESTER HYDROLASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; BACTERIAL PROTEIN; CARBOXYLIC ACID; CYTOCHROME P450;

ANAEROBIC DIGESTION; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CELL GROWTH; CHEMICAL PARAMETERS; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENERGY RESOURCE; ENZYME ACTIVITY; FATTY ACID OMEGA OXIDATION; FATTY ACID OXIDATION; FATTY ACID SYNTHESIS; FERMENTATION; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; METABOLIC ENGINEERING; NONHUMAN; OXIDATION KINETICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION;

ACINETOBACTER SP.; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; CARBOXYLIC ACIDS; CYTOCHROME P-450 ENZYME SYSTEM; METABOLIC ENGINEERING; OXIDATION-REDUCTION; PSEUDOMONAS PUTIDA; THIOLESTER HYDROLASES;

EID: 84922980443     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.01.007     Document Type: Article

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