Analysis of DNA damage via single-cell electrophoresis

Methods in Molecular Biology

Volumn 1054, Issue , 2013, Pages 209-218

  Anderson, Diana ^a   Laubenthal, Julian ^a  

^a UNIVERSITY OF BRADFORD   (United Kingdom)

Author keywords

Alkali labile sites; Comet assay; DNA damage; Double strand breaks; Single cell electrophoresis; Single strand breaks

Indexed keywords

ALKALI; DNA; MUTAGENIC AGENT;

ARTICLE; BIOLOGICAL MONITORING; CELL ISOLATION; CELL NUCLEUS MATRIX; CELL SUSPENSION; COHORT ANALYSIS; COMET ASSAY; DNA DAMAGE; DOUBLE STRANDED DNA BREAK; GENOTOXICITY; PH; PRIORITY JOURNAL; SINGLE STRANDED DNA BREAK; CHEMISTRY; DNA REPAIR; GENETICS; HUMAN; PROCEDURES;

COMET ASSAY; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR; HUMANS; MUTAGENS;

EID: 84922813354     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-565-1_14     Document Type: Article

References (15)

1. Ostling O, Johanson KJ (1984) Microelectrophoretic study of radiation- induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123:291-298
2. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191
3. Olive PL, Banath JP, Durand RE (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay. Radiat Res 122:86-94
4. Klaude M, Eriksson S, Nygren J, Ahnstrom G (1996) The comet assay: Mechanisms and technical considerations. Mutat Res 363:89-96
5. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206-221
6. Fairbairn DW, Olive PL, O'Neill KL (1995) The comet assay: A comprehensive review. Mutat Res 339:37-59
7. Collins AR (2004) The comet assay for DNA damage and repair: Principles, applications, and limitations. Mol Biotechnol 26:249-261
8. Anderson D, Schmid TE, Baumgartner A, Cemeli-Carratala E, Brinkworth MH, Wood JM (2003) Oestrogenic compounds and oxidative stress (in human sperm and lymphocytes in the Comet assay). Mutat Res 544:173-178
9. Sipinen V, Laubenthal J, Baumgartner A, Cemeli E, Linschooten JO, Godschalk RW, Van Schooten FJ, Anderson D, Brunborg G (2010) In vitro evaluation of baseline and induced DNA damage in human sperm exposed to benzo[a]pyrene or its metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide, using the comet assay. Mutagenesis 25: 417-425
10. Cemeli E, Baumgartner A, Anderson D (2009) Antioxidants and the Comet assay. Mutat Res 681:51-67
11. Collins AR, Azqueta A (2011) DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assay. Mutat Res 736(1-2):122-129
12. Baumgartner A, Cemeli E, Anderson D (2009) The comet assay in male reproductive toxicology. Cell Biol Toxicol 25:81-98
13. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DE, Tice R et al (2000) IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutat Res 463:111-172
14. Kumaravel TS, Vilhar B, Faux SP, Jha AN (2009) Comet assay measurements: A perspective. Cell Biol Toxicol 25:53-64
15. Collins AR, Oscoz AA, Brunborg G, Gaivao I, Giovannelli L, Kruszewski M, Smith CC, Stetina R (2008) The comet assay: Topical issues. Mutagenesis 23:143-151