Nuclease-mediated Double-Strand Break (DSB) enhancement of Small Fragment Homologous Recombination (SFHR) gene modification in human-induced pluripotent stem cells (hiPSCs)

Methods in Molecular Biology

Volumn 1114, Issue , 2014, Pages 279-290

  Sargent, R Geoffrey ^a   Suzuki, Shingo ^a,b   Gruenert, Dieter C ^c,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b KUMAMOTO UNIVERSITY   (Japan)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Clonal isolation; Cystic fibrosis; Gene targeting; iPS cells; Nucleofection; Polynucleotides; SDFs; Sequence specific endonucleases; SFHR; TALENs

Indexed keywords

CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; GENOMIC DNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE; DNA FRAGMENT;

AGAR GEL ELECTROPHORESIS; ALLELE; ANIMAL CELL; ARTICLE; CELL EXPANSION; CELL ISOLATION; CONTROLLED STUDY; DNA MODIFICATION; DNA PURIFICATION; DOUBLE STRANDED DNA BREAK; GENE; GENE MUTATION; GENETIC ENHANCEMENT; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; NONHUMAN; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SMALL FRAGMENT HOMOLOGOUS RECOMBINATION GENE; WILD TYPE; DNA SEQUENCE; EXON; HOMOZYGOSITY; NUCLEOTIDE SEQUENCE; SMALL FRAGMENT HOMOLOGOUS RECOMBINATION;

IPS;

CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; ENDONUCLEASES; GENE TARGETING; HOMOLOGOUS RECOMBINATION; HUMANS; INDUCED PLURIPOTENT STEM CELLS;

EID: 84922059115     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-761-7_18     Document Type: Article

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