Comparative performance of the GeneXpert C. difficile PCR assay and C. diff Quik Chek Complete kit assay for detection of Clostridium difficile antigen and toxins in symptomatic community-onset infections

International Journal of Infectious Diseases

Volumn 29, Issue , 2014, Pages e244-e248

  Jamal, Wafaa ^a   Pauline, Eunice M ^a   Rotimi, Vincent O ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Antigen detection; Clostridium difficile; EIA; Gene Xpert; Toxin

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; GLUTAMATE DEHYDROGENASE; BACTERIAL ANTIGEN; BACTERIAL TOXIN; DIAGNOSTIC KIT;

ANTIGEN DETECTION; ARTICLE; CHILD; CLOSTRIDIUM DIFFICILE INFECTION; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME IMMUNOASSAY; FECES ANALYSIS; FECES CULTURE; HUMAN; IMMUNOLOGY TEST KIT; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TOXIN ANALYSIS; COMMUNITY-ACQUIRED INFECTIONS; DIAGNOSTIC KIT; ENTEROCOLITIS, PSEUDOMEMBRANOUS; EVALUATION STUDY; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; PEPTOCLOSTRIDIUM DIFFICILE; POLYMERASE CHAIN REACTION; PROCEDURES;

ANTIGENS, BACTERIAL; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; COMMUNITY-ACQUIRED INFECTIONS; ENTEROCOLITIS, PSEUDOMEMBRANOUS; GLUTAMATE DEHYDROGENASE; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY;

EID: 84921913207     PISSN: 12019712     EISSN: 18783511     Source Type: Journal    
DOI: 10.1016/j.ijid.2014.10.025     Document Type: Article

References (28)

1. Bartlett J.G. Narrative review: the new epidemic of Clostridium difficile-associated enteric disease. Ann Intern Med 2006, 145:758-764.
2. Crobach M.J., Dekkers O.M., Wilcox M.H., Kuijper E.J. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations diagnosing of Clostridium difficile infection (CDI). Clin Microbiol Infect 2009, 15:1053-1066.
3. He M., Miyajima F., Roberts P., Ellison L., Pickard D.J., Martin M.J., et al. Emergence and global spread of epidemic healthcare-associated Clostridium difficile. Nat Genet 2013, 45:109-113.
4. Bauer M.P., Notermans D.W., van Benthem B.H., Brazier J.S., Wilcox M.H., Rupnik M., et al. Clostridium difficile infection in Europe: a hospital base survey. Lancet 2011, 377:63-73.
5. Karas J.A., Enoch D.A., Aliyu S.H. A review of mortality due to Clostridium difficile infection. J infect 2010, 61:1-8.
6. Monaghan T., Boswell T., Mahida Y.R. Recent advances in Clostridium difficile-associated disease. Gut 2008, 57:850-870.
7. Cohen S.H., Gerding D.N., Johnson S., Kelly C.P., Loo V.G., McDonald L.C., et al. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect Control Hosp Epidemiol 2010, 31:431-455.
8. Reller M.E., Lema C.A., Perl T.M., Cai M., Ross T.L., Speck K.A., et al. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J Clin Microbiol 2007, 45:3601-3605.
9. Kvach E.J., Ferguson D., Riska P.F., Landry M.L. Comparison of BD GenOhm C diff real-time PCR assay with two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J Clin Microbiol 2010, 48:109-114.
10. Swindells J., Brenwald N., Reading N., Oppenheim B. Evaluation of diagnostic tests for Clostridium difficile infection. J Clin Microbiol 2010, 48:606-608.
11. Wilcox M.H. Overcoming barriers to effective recognition and diagnosis of Clostridium difficile infection. Clin Microbiol Infect 2012, 18(Suppl 6):13-20.
12. Delmee M., Van Broeck J., Simon A., Janssens M., Avesani V. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J Med Microbiol 2005, 54:187-191.
13. Gerding D.N. Global epidemiology of Clostridium difficile infection in 2010. Infect Control Hosp Epidemiol 2010, 31(Suppl 1):S32-S34.
14. Hansen G., Blatt S., Brecher S.M., Dubberke E., Dorsett P. Clinical laboratory news: Clostridium difficile. Clinical Laboratory News 2010, 36:10-13.
15. Alcala L., Sanchez-Cambronero L., Catalan M.P., Sanchez-Somolinos M., Pelaez M.T., Marin M., et al. Comparison of three commercial methods for rapid detection of Clostridium difficile toxins A and B from fecal specimens. J Clin Microbiol 2008, 46:3833-3835.
16. Eastwood K., Else P., Charlett A., Wilcox M. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytogenic culture methods. J Clin Microbiol 2009, 47:3211-3217.
17. Miendje Deyi V.Y., Vandenberg O., Mascart G., Gning S., Retore P., Douat N., et al. Diagnostic value of five commercial tests for the rapid diagnosis of Clostridium difficile-associated disease. Clin Lab 2008, 54:9-13.
18. Hernandez-Rocha C., Barra-Carrasco J., Alvarez-Lobos M., Paredes-Sabja D., Guzman-Duran A.M. Prospective comparison of a commercial multiplex real-time polymerase chain reaction and an enzyme immunoassay with toxigenic culture in the diagnosis of Clostridium difficile-associated infections. Diagn Microbiol Infect Dis 2013, 75:361-365.
19. Quinn C.D., Sefers S.E., Babiker W., He Y., Alcabasa R., Stratton C.W., et al. C. diff Quik Chek Complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J Clin Microbiol 2010, 48:603-605.
20. Rotimi V.O., Mokaddas E.M., Jamal W.Y., Verghese T.L., El-Din K., Junaid T.A. Hospital-acquired Clostridium difficile infection amongst ICU and burn patients in Kuwait. Med Princ Pract 2002, 11:23-28.
21. Tenover F.C., Novak-Weekley S., Woods C.W., Peterson L.R., Davis T., Schreckenberger P., et al. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J Clin Microbiol 2010, 48:3719-3724.
22. Leslie J.L., Cohen S.H., Solnick J.V., Polage C.R. Role of fecal Clostridium difficile load in discrepancies between toxin tests and PCR: is quantitation the next step in C. difficile testing?. Eur J Clin Microbiol Infect Dis 2012, 31:3295-3299.
23. Curry S.R., Schlackman J.L., Hamilton T.M., Henderson T.K., Brown N.T., Marsh J.W., et al. Perirectal swab surveillance for Clostridium difficile by use of selective broth preamplification and real-time PCR detection of tcdB. J Clin Microbiol 2011, 49:3788-3793.
24. Emmadi R., Boonyaratanakornkit J.B., Selvarangan R., Shyamala V., Zimmer B.L., Williams L., et al. Molecular methods and platforms for infectious diseases testing-a review of FDA-approved and cleared assays. J Mol Diagn 2011, 13:583-604.
25. Dalpke A.H., Hofko M., Zorn M., Zimmermann S. Evaluation of the fully automated BD MAX Cdiff and Xpert C. difficile assays for direct detection of Clostridium difficile in stool specimens. J Clin Microbiol 2013, 51:1906-1908.
26. Dubberke E.R., Han Z., Bobo L., Hink T., Lawrence B., Copper S., et al. Impact of clinical symptoms on interpretation of diagnostic assays for Clostridium difficile. J Clin Microbiol 2011, 49:2887-2893.
27. Planche T.D., Davies K.A., Coen P.G., Finney J.M., Monahan I.M., Morris K.A., et al. Differences in outcome according to Clostridium difficile testing method: a prospective multicenter diagnostic validation study of C. difficile infection. Lancet Infect Dis 2013, 13:936-945.
28. Jamal W., Rotimi V.O., Brazier J., Duerden B.I. Analysis of prevalence, risk factors and molecular epidemiology of Clostridium difficile infection in Kuwait over a 3-year period. Anaerobe 2010, 16:560-565.