Evaluation of the fully automated BD MAX Cdiff and Xpert C. difficile assays for direct detection of Clostridium difficile in stool specimens

Journal of Clinical Microbiology

Volumn 51, Issue 6, 2013, Pages 1906-1908

  Dalpke, Alexander H ^a   Hofko, Marjeta ^a   Zorn, Markus ^a   Zimmermann, Stefan ^a  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM TOXIN;

AGAR MEDIUM; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BIOASSAY; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; DIAGNOSTIC TEST ACCURACY STUDY; ENDOTOXIN DETECTION; FECES ANALYSIS; HUMAN; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; MAJOR CLINICAL STUDY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

AUTOMATION, LABORATORY; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CHROMOGENIC COMPOUNDS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CULTURE MEDIA; FECES; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

CLOSTRIDIUM DIFFICILE;

EID: 84878184870     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00344-13     Document Type: Article

References (25)

1. Rupnik M, Wilcox MH, Gerding DN. 2009. Clostridium difficile infection: new developments in epidemiology and pathogenesis. Nat. Rev. Microbiol. 7:526-536.
2. Crobach MJ, Dekkers OM, Wilcox MH, Kuijper EJ. 2009. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile infection (CDI). Clin. Microbiol. Infect. 15:1053-1066.
3. Swindells J, Brenwald N, Reading N, Oppenheim B. 2010. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 48:606-608.
4. Wilcox MH, Planche T, Fang FC, Gilligan P. 2010. What is the current role of algorithmic approaches for diagnosis of Clostridium difficile infection? J. Clin. Microbiol. 48:4347-4353.
5. Peterson LR, Mehta MS, Patel PA, Hacek DM, Harazin M, Nagwekar PP, Thomson RB, Jr, Robicsek A. 2011. Laboratory testing for Clostridium difficile infection: light at the end of the tunnel. Am. J. Clin. Pathol. 136:372-380.
6. Belanger SD, Boissinot M, Clairoux N, Picard FJ, Bergeron MG. 2003. Rapid detection of Clostridium difficile in feces by real-time PCR. J. Clin. Microbiol. 41:730-734. (Pubitemid 36184188)
7. Le Guern R, Herwegh S, Grandbastien B, Courcol R, Wallet F. 2012. Evaluation of a new molecular test, the BD Max Cdiff, for detection of toxigenic Clostridium difficile in fecal samples. J. Clin. Microbiol. 50:3089-3090.
8. Carroll KC. 2011. Tests for the diagnosis of Clostridium difficile infection: the next generation. Anaerobe 17:170-174.
9. Eckert C, Burghoffer B, Lalande V, Barbut F. 2013. Evaluation of the chromogenic agar chromID C. difficile. J. Clin. Microbiol. 51:1002-1004.
10. Perry JD, Asir K, Halimi D, Orenga S, Dale J, Payne M, Carlton R, Evans J, Gould FK. 2010. Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours. J. Clin. Microbiol. 48:3852-3858.
11. Fournier R, Wallet F, Grandbastien B, Dubreuil L, Courcol R, Neut C, Dessein R. 2012. Chemical extraction versus direct smear for MALDITOF mass spectrometry identification of anaerobic bacteria. Anaerobe 18:294-297.
12. Spigaglia P, Mastrantonio P. 2002. Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates. J. Clin. Microbiol. 40:3470-3475. (Pubitemid 34977470)
13. Arroyo LG, Rousseau J, Willey BM, Low DE, Staempfli H, McGeer A, Weese JS. 2005. Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions. J. Clin. Microbiol. 43:5341-5343. (Pubitemid 41484055)
14. Buchan BW, Mackey TL, Daly JA, Alger G, Denys GA, Peterson LR, Kehl SC, Ledeboer NA. 2012. Multicenter clinical evaluation of the portrait toxigenic C. difficile assay for detection of toxigenic Clostridium difficile strains in clinical stool specimens. J. Clin. Microbiol. 50:3932-3936.
15. Huang H, Weintraub A, Fang H, Nord CE. 2009. Comparison of a commercial multiplex real-timePCRto the cell cytotoxicity neutralization assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 47:3729-3731.
16. Novak-Weekley SM, Marlowe EM, Miller JM, Cumpio J, Nomura JH, Vance PH, Weissfeld A. 2010. Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J. Clin. Microbiol. 48:889-893.
17. Shin S, Kim M, Lim H, Kim H, Lee K, Chong Y. 2012. Evaluation of the Xpert Clostridium difficile assay for the diagnosis of Clostridium difficile infection. Ann. Lab. Med. 32:355-358.
18. Viala C, Le Monnier A, Maataoui N, Rousseau C, Collignon A, Poilane I. 2012. Comparison of commercial molecular assays for toxigenic Clostridium difficile detection in stools: BD GeneOhm Cdiff, XPert C. difficile and illumigene C. difficile. J. Microbiol. Methods 90:83-85.
19. Chapin KC, Dickenson RA, Wu F, Andrea SB. 2011. Comparison of five assays for detection of Clostridium difficile toxin. J. Mol. Diagn. 13:395-400.
20. Eastwood K, Else P, Charlett A, Wilcox M. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a realtime PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:3211-3217.
21. Fenner I, Eigner U, Lensing C, Petersen H, Veldenzer A, Wild U, Holfelder M. 2011. Evaluation of six real-time PCR-methods for the detection of Clostridium difficile in stool specimens. Abstr. 21st Eur. Congr. Clin. Microbiol. Infect. Dis., poster P1988.
22. Nistico JA, Hage JE, Schoch PE, Cunha BA. 2013. Unnecessary repeat Clostridium difficile PCR testing in hospitalized adults with C. difficile-negative diarrhea. Eur. J. Clin. Microbiol. Infect. Dis. 32:97-99.
23. Tenover FC, Baron EJ, Peterson LR, Persing DH. 2011. Laboratory diagnosis of Clostridium difficile infection can molecular amplification methods move us out of uncertainty? J. Mol. Diagn. 13:573-582.
24. Dubberke ER, Han Z, Bobo L, Hink T, Lawrence B, Copper S, Hoppe- Bauer J, Burnham CA, Dunne WM, Jr. 2011. Impact of clinical symptoms on interpretation of diagnostic assays for Clostridium difficile infections. J. Clin. Microbiol. 49:2887-2893.
25. Tenover FC, Novak-Weekley S, Woods CW, Peterson LR, Davis T, Schreckenberger P, Fang FC, Dascal A, Gerding DN, Nomura JH, Goering RV, Akerlund T, Weissfeld AS, Baron EJ, Wong E, Marlowe EM, Whitmore J, Persing DH. 2010. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 48:3719-3724.