Live spheroid formation recorded with light sheet-based fluorescence microscopy

Methods in Molecular Biology

Volumn 1251, Issue , 2014, Pages 43-57

  Pampaloni, Francesco ^a   Richa, Roli ^a   Stelzer, Ernst H K ^a  

^a NONE

Author keywords

HepaRG cells; Light sheet based fluorescence microscopy; Live cell assay; Live cell imaging; LSFM; Spheroid formation; SPIM; Three dimensional cell cultures; Tumor spheroids

Indexed keywords

H2B GFP PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN; HISTONE;

ARTICLE; CELL AGGREGATION; CELL PROLIFERATION; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HEPARG CELL LINE; HUMAN; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; SPHEROID CELL; THREE DIMENSIONAL IMAGING; CELL ADHESION; CELL LINE; GENETICS; IMAGE PROCESSING; MULTICELLULAR SPHEROID; PHYSIOLOGY; PROCEDURES; ULTRASTRUCTURE;

CELL ADHESION; CELL LINE; CELL PROLIFERATION; GREEN FLUORESCENT PROTEINS; HISTONES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, FLUORESCENCE; SPHEROIDS, CELLULAR;

EID: 84921801432     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2080-8_3     Document Type: Article

References (24)

1. Baker M (2010) Cellular imaging: taking a long, hard look. Nature 466:1137–1140
2. Homem CCF, Reichardt I, Berger C et al (2013) Long-term live cell imaging and automated 4D analysis of drosophila neuroblast lineages. PloS One 8(11):e79588. doi: 10.1371/journal.pone.0079588
3. Carlton PM, Boulanger J, Kervrann C et al (2010) Fast live simultaneous multiwavelength four-dimensional optical microscopy. Proc Natl Acad Sci U S A 107:16016–16022
4. Huisken J, Stainier DY (2009) Selective plane illumination microscopy techniques in developmental biology. Development 136: 1963–1975
5. Huisken J, Swoger J, Del Bene F et al (2004) Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305:1007–1009
6. Keller PJ, Schmidt AD, Wittbrodt J et al (2008) Reconstruction of zebrafi sh early embryonic development by scanned light sheet microscopy. Science 322:1065–1069
7. Keller PJ, Stelzer EH (2008) Quantitative in vivo imaging of entire embryos with digital scanned laser light sheet fl uorescence microscopy. Curr Opin Neurobiol 18:624–632
8. Maizel A, von Wangenheim D, Federici F et al (2011) High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fl uorescence microscopy. Plant J 68:377–385
9. Keller PJ, Schmidt AD, Santella A et al (2010) Fast, high-contrast imaging of animal development with scanned light sheet-based structured- illumination microscopy. Nat Methods 7:637–642
10. Pampaloni F, Kroschewski R, Berge U et al (2014) Tissue-Culture Light Sheet Fluorescence Microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions. Integrative Biology. doi: 10.1039/C4IB00121D
11. Harma V, Virtanen J, Makela R et al (2010) A comprehensive panel of three-dimensional models for studies of prostate cancer growth, invasion and drug responses. PLoS One 5:e10431
12. Friedrich J, Seidel C, Ebner R et al (2009) Spheroid-based drug screen: considerations and practical approach. Nat Protoc 4:309–324
13. Matsuda M, Shiratori S (2011) Correlation of antithrombogenicity and heat treatment for layer-by-layer self-assembled polyelectrolyte fi lms. Langmuir 27:4271–4277
14. Takano S, Tian W, Matsuda M et al (2011) Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing. Brain Tumor Pathol 28:115–123
15. Lee MY, Kumar RA, Sukumaran SM et al (2008) Three-dimensional cellular microarray for high-throughput toxicology assays. Proc Natl Acad Sci U S A 105:59–63
16. Goto A, Hoshino M, Matsuda M et al (2011) Phosphorylation of STEF/Tiam2 by protein kinase A is critical for Rac1 activation and neurite outgrowth in dibutyryl cAMPtreated PC12D cells. Mol Biol Cell 22: 1780–1790
17. Kelm JM, Timmins NE, Brown CJ et al (2003) Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol Bioeng 83:173–180
18. Lin RZ, Chou LF, Chien CC et al (2006) Dynamic analysis of hepatoma spheroid formation: roles of E-cadherin and beta1-integrin. Cell Tissue Res 324:411–422
19. Verveer PJ, Swoger J, Pampaloni F et al (2007) High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy. Nat Methods 4:311–313
20. Lorenzo C, Frongia C, Jorand R et al (2011) Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy. Cell Div 6:22
21. Guillouzo A, Corlu A, Aninat C et al (2007) The human hepatoma HepaRG cells: a highly differentiated model for studies of liver metabolism and toxicity of xenobiotics. Chem Biol Interact 168:66–73
22. Greger K, Swoger J, Stelzer EH (2007) Basic building units and properties of a fl uorescence single plane illumination microscope. Rev Sci Instrum 78:023705
23. Huisken J, Stainier DY (2007) Even fl uorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Opt Lett 32:2608–2610
24. Zeng J, Du J, Lin J et al (2009) High-effi ciency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors. Mol Ther 17:1585–1593