Genomic high-resolution profiling of single CKpos/CD45neg flow-sorting purified circulating tumor cells from patients with metastatic breast cancer

Clinical Chemistry

Volumn 60, Issue 10, 2014, Pages 1290-1297

  Neves, Rui P L ^a   Raba, Katharina ^a   Schmidt, Oliver ^b   Honisch, Ellen ^a   Meier Stiegen, Franziska ^a   Behrens, Bianca ^a   Möhlendick, Birte ^a   Fehm, Tanja ^a   Neubauer, Hans ^a   Klein, Christoph A ^b,c   Polzer, Bernhard ^c   Sproll, Christoph ^a   Fischer, Johannes C ^a   Niederacher, Dieter ^a   Stoecklein, Nikolas H ^a  

^a UNIVERSITY HOSPITAL DÜSSELDORF   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

^c FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD45 ANTIGEN; CYCLIN D1; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE 3 KINASE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE CATALYTIC SUBUNIT ALPHA; PROTEIN P53; UNCLASSIFIED DRUG; ALLOPHYCOCYANIN; CCND1 PROTEIN, HUMAN; PHOSPHATIDYLINOSITOL 3 KINASE; PHYCOCYANIN; PHYCOERYTHRIN; PIK3CA PROTEIN, HUMAN; PTPRC PROTEIN, HUMAN; TP53 PROTEIN, HUMAN; TUMOR MARKER;

ADVANCED CANCER; ARTICLE; BREAST METASTASIS; CANCER PATIENT; CELL LEVEL; CELL SORTER; CHROMOSOME ABERRATION; CIRCULATING TUMOR CELL; CLINICAL ARTICLE; COMPARATIVE GENOMIC HYBRIDIZATION; CORRELATIONAL STUDY; EXON; GENE AMPLIFICATION; GENE LOCUS; GENE SEQUENCE; GENOME; GENOMICS; HUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE STUDY; VALIDATION STUDY; WORKFLOW; BLOOD; BREAST TUMOR; COPY NUMBER VARIATION; FEMALE; FLOW CYTOMETRY; GENETICS; METABOLISM; METASTASIS; MUTATION; PATHOLOGY; PROCEDURES; SINGLE CELL ANALYSIS; TUMOR EMBOLISM;

ANTIGENS, CD45; BREAST NEOPLASMS; COMPARATIVE GENOMIC HYBRIDIZATION; CYCLIN D1; DNA COPY NUMBER VARIATIONS; EXONS; FEMALE; FLOW CYTOMETRY; HUMANS; MUTATION; NEOPLASM METASTASIS; NEOPLASTIC CELLS, CIRCULATING; PHOSPHATIDYLINOSITOL 3-KINASES; PHYCOCYANIN; PHYCOERYTHRIN; SINGLE-CELL ANALYSIS; TUMOR MARKERS, BIOLOGICAL; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84921793301     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.222331     Document Type: Article

References (29)

1. Devriese LA, Voest EE, Beijnen JH, Schellens JH. Circulating tumor cells as pharmacodynamic bio-marker in early clinical oncological trials. Cancer Treat Rev 2011;37:579-89.
2. Kaiser J. Medicine. Cancer's circulation problem. Science 2010;327:1072-4.
3. van de Stolpe A, Pantel K, Sleijfer S, Terstappen LW, den Toonder JM. Circulating tumor cell isolation and diagnostics: toward routine clinical use. Cancer Res 2011;71:5955-60.
4. Fischer JC, Niederacher D, Topp SA, Honisch E, Schumacher S, Schmitz N, et al. Diagnostic leu-kapheresis enables reliable detection of circulating tumor cells of nonmetastatic cancer patients. Proc Natl Acad Sci USA 2013;110:16580-5.
5. Campbell PJ, Yachida S, Mudie LJ, Stephens PJ, Pleasance ED, Stebbings LA, et al. The patterns and dynamics of genomic instability in metastatic pancreatic cancer. Nature 2010;467:1109-13.
6. Goranova TE, Ohue M, Shimoharu Y, Kato K. Dynamics of cancer cell subpopulations in primary and metastatic colorectal tumors. Clin Exp Metastasis 2011;28:427-35.
7. Klein CA. Selection and adaptation during meta-static cancer progression. Nature 2013;501:365-72.
8. Vermaat JS, Nijman IJ, Koudijs MJ, Gerritse FL, Scherer SJ, Mokry M, et al. Primary colorectal cancers and their subsequent hepatic metastases are genetically different: implications for selection of patients for targeted treatment. Clin Cancer Res 2012;18:688-99.
9. Stoecklein NH, Klein CA. Genetic disparity between primary tumours, disseminated tumour cells, and manifest metastasis. Int J Cancer 2010; 126:589-98.
10. Racila E, Euhus D, Weiss AJ, Rao C, McConnell J, Terstappen LW, Uhr JW. Detection and characterization of carcinoma cells in the blood. Proc Natl Acad Sci USA 1998;95:4589-94.
11. Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, et al. Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res 2004;10:6897-904.
12. Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, et al. Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med 2004;351:781-91.
13. Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, et al. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. Nat Biotechnol 2013;31:539-44.
14. Lianidou ES, Mavroudis D, Georgoulias V. Clinical challenges in the molecular characterization of circulating tumour cells in breast cancer. Br J Cancer 2013;108:2426-32.
15. Swennenhuis JF, Reumers J, Thys K, Aerssens J, Terstappen LW. Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACs. Genome Med 2013;5:106.
16. Mohlendick B, Bartenhagen C, Behrens B, Honisch E, Raba K, Knoefel WT, Stoecklein NH. A robust method to analyze copy number alterations of less than 100 kb in single cells using oligonucleotide array CGH. PLoS One 2013;8: e67031.
17. Klein CA, Schmidt-Kittler O, Schardt JA, Pantel K, Speicher MR, Riethmuller G. Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proc Natl Acad Sci USA 1999;96:4494-9.
18. Gillett C, Fantl V, Smith R, Fisher C, Bartek J, Dickson C, et al. Amplification and overexpres-sion of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Res 1994; 54:1812-7.
19. Buckley MF, Sweeney KJ, Hamilton JA, Sini RL, Manning DL, Nicholson RI, et al. Expression and amplification of cyclin genes in human breast cancer. Oncogene 1993;8:2127-33.
20. Bamford S, Dawson E, Forbes S, Clements J, Pettett R, Dogan A, et al. The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website. Br J Cancer 2004;91:355-8.
21. Gasch C, Bauernhofer T, Pichler M, Langer-Freitag S, Reeh M, Seifert AM, et al. Heterogeneity of epidermal growth factor receptor status and mutations of KRAS/PIK3CA in circulating tumor cells of patients with colorectal cancer. Clin Chem 2013;59:252-60.
22. Riethdorf S, Muller V, Zhang L, Rau T, Loibl S, Komor M, et al. Detection and HER2 expression of circulating tumor cells: prospective monitoring in breast cancer patients treated in the neoadju-vant GeparQuattro trial. Clin Cancer Res 2010; 16:2634-45.
23. Heitzer E, Auer M, Gasch C, Pichler M, Ulz P, Hoffmann EM, et al. Complex tumor genomes inferred from single circulating tumor cells by array-CGH and next-generation sequencing. Cancer Res 2013;73:2965-75.
24. Peeters DJ, De Laere B, Van den Eynden GG, Van Laere SJ, Rothe F, Ignatiadis M, et al. Semiauto-mated isolation and molecular characterisation of single or highly purified tumour cells from Cell-Search enriched blood samples using dielectro-phoretic cell sorting. Br J Cancer 2013;108:1358-67.
25. Stoecklein NH, Erbersdobler A, Schmidt-Kittler O, Diebold J, Schardt JA, Izbicki JR, Klein CA. SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples. Am J Pathol 2002;161: 43-51.
26. van Beers EH, Joosse SA, Ligtenberg MJ, Fles R, Hogervorst FB, Verhoef S, Nederlof PM. A multiplex PCR predictor for aCGH success of FFPE samples. Br J Cancer 2006;94:333-7.
27. Baudis M, Cleary ML. Progenetix.net: an online repository for molecular cytogenetic aberration data. Bioinformatics 2001;17:1228-9.
28. Schmidt-Kittler O, Ragg T, Daskalakis A, Granzow M, Ahr A, Blankenstein TJ, et al. From latent disseminated cells to overt metastasis: genetic analysis of systemic breast cancer progression. Proc Natl Acad Sci USA 2003;100:7737-42.
29. Klein CA, Blankenstein TJ, Schmidt-Kittler O, Petronio M, Polzer B, Stoecklein NH, Riethmuller G. Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet 2002;360:683-9.