Dual-Color Ultrasensitive Bright-Field RNA in Situ Hybridization with Rnascope

Methods in Molecular Biology

Volumn 1211, Issue , 2014, Pages 139-149

  Wang, Hongwei ^a   Su, Nan ^a   Wang, Li Chong ^a   Wu, Xingyong ^a   Bui, Son ^a   Nielsen, Allissa ^a   Vo, Hong Thuy ^a   Luo, Yuling ^a   Ma, Xiao Jun ^a  

^a NONE

Author keywords

Biomarker; Cancer; Dual color CISH; Gene expression; In situ hybridization; Messenger; Nucleic acid hybridization; RNA; RNAscope

Indexed keywords

ALKALINE PHOSPHATASE; HORSERADISH PEROXIDASE; RNA; MESSENGER RNA;

ARTICLE; CONTROLLED STUDY; IN SITU HYBRIDIZATION; PRIORITY JOURNAL; STAINING; TISSUE PREPARATION; TISSUE SECTION; ANIMAL; GENETICS; HUMAN; MICROSCOPY; MOUSE; NEOPLASM; PATHOLOGY; PROCEDURES;

ANIMALS; HUMANS; IN SITU HYBRIDIZATION; MICE; MICROSCOPY; NEOPLASMS; RNA, MESSENGER;

EID: 84921767559     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1459-3_12     Document Type: Article

References (34)

1. Itzkovitz S, van Oudenaarden A (2011) Validating transcripts with probes and imaging technology. Nat Methods 8:S12–S19
2. Bartlett JM, Campbell FM, Ibrahim M et al (2011) A UK NEQAS ISH multicenter ring study using the Ventana HER2 dualcolor ISH assay. Am J Clin Pathol 135: 157–162
3. Mollerup J, Henriksen U, Müller S et al (2012) Dual color chromogenic in situ hybridization for determination of HER2 status in breast cancer: a large comparative study to current state of the art fluorescence in situ hybridization. BMC Clin Pathol 12:3
4. Tubbs RR, Wang H, Wang Z et al (2013) Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. Am J Clin Pathol 140:736–746
5. Wang F, Flanagan J, Su N et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14:22–29
6. Tanas MR, Sboner A, Oliveira AM et al (2011) Identification of a disease-defining gene fusion in epithelioid hemangioendothelioma. Sci Transl Med 3:98ra82
7. Bordeaux JM, Cheng H, Welsh AW et al (2012) Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen. PLoS One 7:e36559
8. Hanley MB, Lomas W, Mittar D et al (2013) Detection of low abundance RNA molecules in individual cells by flow cytometry. PLoS One 8:e57002
9. Wang Z, Portier BP, Gruver AM et al (2013) Automated quantitative RNA in situ hybridization for resolution of equivocal and heterogeneous ERBB2 (HER2) status in invasive breast carcinoma. J Mol Diagn 15:210–219
10. Mehrad M, Carpenter DH, Chernock RD et al (2013) Papillary squamous cell carcinoma of the head and neck: clinicopathologic and molecular features with special reference to human papillomavirus. Am J Surg Pathol 37: 1349–1356
11. Schache AG, Liloglou T, Risk JM et al (2013) Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma. Br J Cancer 108:1332–1339
12. Bishop JA, Ma XJ, Wang H et al (2012) Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. Am J Surg Pathol 36:1874–1882
13. Liu X, Bates R, Yin DM et al (2011) Specific regulation of NRG1 isoform expression by neuronal activity. J Neurosci 31:8491–8501
14. Hickman S, Kingery N, Ohsumi T et al (2013) The microglial sensome revealed by direct RNA sequencing. Nat Neurosci 16:1896–1905
15. Lewis JS Jr, Chernock RD, Ma XJ et al (2012) Partial p16 staining in oropharyngeal squamous cell carcinoma: extent and pattern correlate with human papillomavirus RNA status. Mod Pathol 25:1212–1220
16. Payne RE, Wang F, Su N et al (2012) Viable circulating tumour cell detection using multiplex RNA in situ hybridisation predicts progression- free survival in metastatic breast cancer patients. Br J Cancer 106:1790–1797
17. Ukpo OC, Flanagan JJ, Ma XJ et al (2011) High-risk human papillomavirus E6/E7 mRNA detection by a novel in situ hybridization assay strongly correlates with p16 expression and patient outcomes in oropharyngeal squamous cell carcinoma. Am J Surg Pathol 35: 1343–1350
18. Staudt ND, Jo M, Hu J et al (2013) Myeloid cell receptor LRP1/CD91 regulates monocyte recruitment and angiogenesis in tumors. Cancer Res 73:3902–3912
19. Burd CE, Sorrentino JA, Clark KS et al (2013) Monitoring tumorigenesis and senescence in vivo with a p16INK4a-luciferase model. Cell 152:340–351
20. Shames DS, Carbon J, Walter K et al (2013) High heregulin expression is associated with activated HER3 and may define an actionable biomarker in patients with squamous cell carcinomas of the head and neck. PLoS One 8:e56765
21. Gao G, Chernock RD, Gay HA et al (2013) A novel RT-PCR method for quantification of human papillomavirus transcripts in archived tissues and its application in oropharyngeal cancer prognosis. Int J Cancer 132:882–890
22. Kim MA, Jung JE, Lee HE et al (2013) In situ analysis of HER2 mRNA in gastric carcinoma: comparison with fluorescence in situ hybridization, dual-color silver in situ hybridization, and immunohistochemistry. Hum Pathol 44: 487–494
23. Ziskin JL, Dunlap D, Yaylaoglu M et al (2013) In situ validation of an intestinal stem cell signature in colorectal cancer. Gut 62:1012–1023
24. Warrick JI, Tomlins SA, Carskadon SL et al (2013) Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization—a correlative study with urine PCA3 and TMPRSS2-ERG. Mod Pathol 27:609–620
25. van Beelen Granlund A, Østvik AE, Brenna Ø et al (2013) REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridization. Cell Tissue Res 352:639–646
26. Ouwendijk WJ, Abendroth A, Traina-Dorge V et al (2013) T-cell infiltration correlates with CXCL10 expression in ganglia of cynomolgus macaques with reactivated simian varicella virus. J Virol 87:2979–2982
27. Sørdal Ø, Qvigstad G, Nordrum IS et al (2013) In situ hybridization in human and rodent tissue by the use of a new and simplified method. Appl Immunohistochem Mol Morphol 21:185–189
28. Takata S, Sawa Y, Uchiyama T et al (2013) Expression of toll-like receptor 4 in glomerular endothelial cells under diabetic conditions. Acta Histochem Cytochem 46:35–42
29. Shinohara DB, Vaghasia AM, Yu SH et al (2013) A mouse model of chronic prostatic inflammation using a human prostate cancerderived isolate of Propionibacterium acnes. Prostate 73:1007–1015
30. Safronetz D, Prescott J, Haddock E et al (2013) Hamster-adapted Sin Nombre virus causes disseminated infection and efficiently replicates in pulmonary endothelial cells without signs of disease. J Virol 87:4778–4782
31. Brenna Ø, Furnes MW, Drozdov I et al (2013) Relevance of TNBS-colitis in rats: a methodological study with endoscopic, histologic and Transcriptomic characterization and correlation to IBD. PLoS One 8:e54543
32. Barry ER, Morikawa T, Butler BL et al (2013) Restriction of intestinal stem cell expansion and the regenerative response by YAP. Nature 493:106–110
33. Yan KS, Chia LA, Li X et al (2012) The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proc Natl Acad Sci U S A 109:466–471
34. Hammond ME, Hayes DF, Dowsett M et al (2010) American Society of Clinical Oncology/ College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. Arch Pathol Lab Med 134: 907–922