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Volumn 1160, Issue , 2014, Pages 4-17

Minimum information necessary for quantitative real-time PCR experiments

Author keywords

Diagnostics; Gene expression; PCR; Reverse transcription

Indexed keywords

ARTICLE; BASIC RESEARCH; CLINICAL PRACTICE; GENE EXPRESSION; PRACTICE GUIDELINE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCIENTIFIC LITERATURE; GENE EXPRESSION PROFILING; PROCEDURES;

EID: 84921424981     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0733-5_2     Document Type: Article
Times cited : (66)

References (26)
  • 1
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
    • Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622
    • (2009) Clin Chem , vol.55 , pp. 611-622
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3
  • 2
    • 84927772485 scopus 로고    scopus 로고
    • Why there is no link between measles virus and autism
    • In: Fitzgerald M, Intech. Rijeka, Croatia
    • Bustin SA (2013) Why there is no link between measles virus and autism. In: Fitzgerald M (ed) Recent advances in autism spectrum disorders, vol I, Intech. Rijeka, Croatia, pp 81–98
    • (2013) Recent Advances in Autism Spectrum Disorders , vol.1 , pp. 81-98
    • Bustin, S.A.1
  • 4
    • 84871817784 scopus 로고    scopus 로고
    • Researchers fi nd no link between XMRV and chronic fatigue syndrome
    • Roehr B (2012) Researchers fi nd no link between XMRV and chronic fatigue syndrome. BMJ 345:e6331
    • (2012) BMJ , vol.6331 , pp. 345
    • Roehr, B.1
  • 6
    • 77649233414 scopus 로고    scopus 로고
    • Why the need for qPCR publication guidelines?—The case for MIQE
    • Bustin SA (2010) Why the need for qPCR publication guidelines?—The case for MIQE. Methods 50:217–226
    • (2010) Methods , vol.50 , pp. 217-226
    • Bustin, S.A.1
  • 7
    • 84941111200 scopus 로고    scopus 로고
    • Apple store, apple or
    • Abdel Nour AM, Pfaffl M (2011) Apple store, apple https://itunes.apple.com/app/miqeqpcr/id423650002?mt=8 or https://play.google.com/store/apps/details?id=com.biorad.miqeqPCR
    • (2011)
    • Abdel Nour, A.M.1    Pfaffl, M.2
  • 8
    • 84878650332 scopus 로고    scopus 로고
    • Announcement: Reducing our irreproducibility
    • Anonymous
    • Anonymous (2013) Announcement: reducing our irreproducibility. Nature 496:398
    • (2013) Nature , vol.496 , pp. 398
  • 9
    • 77950523190 scopus 로고    scopus 로고
    • Routine lab method’s accuracy called into question
    • Shaffer C (2010) Routine lab method’s accuracy called into question. Nat Med 16:349
    • (2010) Nat Med , vol.16 , pp. 349
    • Shaffer, C.1
  • 10
    • 84877260561 scopus 로고    scopus 로고
    • PCR: Living life amplifi ed and standardized
    • Marx V (2013) PCR: living life amplifi ed and standardized. Nat Methods 10:391–395
    • (2013) Nat Methods , vol.10 , pp. 391-395
    • Marx, V.1
  • 11
    • 77956673889 scopus 로고    scopus 로고
    • MIQE precis: Practical implementation of minimum standard guidelines for fl uorescencebased quantitative real-time PCR experiments
    • Bustin SA, Beaulieu JF, Huggett J et al (2010) MIQE precis: practical implementation of minimum standard guidelines for fl uorescencebased quantitative real-time PCR experiments. BMC Mol Biol 11:74
    • (2010) BMC Mol Biol , vol.11 , pp. 74
    • Bustin, S.A.1    Beaulieu, J.F.2    Huggett, J.3
  • 12
    • 84878431927 scopus 로고    scopus 로고
    • The digital MIQE guidelines: Minimum information for publication of quantitative digital PCR experiments
    • Huggett JF, Foy CA, Benes V et al (2013) The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments. Clin Chem 59:892–902
    • (2013) Clin Chem , vol.59 , pp. 892-902
    • Huggett, J.F.1    Foy, C.A.2    Benes, V.3
  • 13
    • 79957717050 scopus 로고    scopus 로고
    • Primer sequence disclosure: A clarifi cation of the MIQE guidelines
    • Bustin SA, Benes V, Garson JA et al (2011) Primer sequence disclosure: a clarifi cation of the MIQE guidelines. Clin Chem 57:919–921
    • (2011) Clin Chem , vol.57 , pp. 919-921
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3
  • 14
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the reverse transcription reaction in mRNA quantifi cation
    • Stahlberg A, Hakansson J, Xian X et al (2004) Properties of the reverse transcription reaction in mRNA quantifi cation. Clin Chem 50: 509–515
    • (2004) Clin Chem , vol.50 , pp. 509-515
    • Stahlberg, A.1    Hakansson, J.2    Xian, X.3
  • 15
    • 4344564046 scopus 로고    scopus 로고
    • Comparison of reverse transcriptases in gene expression analysis
    • Stahlberg A, Kubista M, Pfaffl M (2004) Comparison of reverse transcriptases in gene expression analysis. Clin Chem 50: 1678–1680
    • (2004) Clin Chem , vol.50 , pp. 1678-1680
    • Stahlberg, A.1    Kubista, M.2    Pfaffl, M.3
  • 16
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A qPCR assay for the detection of inhibitors in nucleic acid preparations
    • Nolan T, Hands RE, Ogunkolade BW et al (2006) SPUD: a qPCR assay for the detection of inhibitors in nucleic acid preparations. Anal Biochem 351:308–310
    • (2006) Anal Biochem , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, B.W.3
  • 17
    • 65549134904 scopus 로고    scopus 로고
    • Differential susceptibility of PCR reactions to inhibitors: An important and unrecognized phenomenon
    • Huggett JF, Novak T, Garson JA et al (2008) Differential susceptibility of PCR reactions to inhibitors: an important and unrecognized phenomenon. BMC Res Notes 1:70
    • (2008) BMC Res Notes , vol.1 , pp. 70
    • Huggett, J.F.1    Novak, T.2    Garson, J.A.3
  • 18
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantifi cation of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin SA (2000) Absolute quantifi cation of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169–193
    • (2000) J Mol Endocrinol , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 19
    • 79961014660 scopus 로고    scopus 로고
    • Standardisation and reporting for nucleic acid quantifi cation
    • Huggett J, Bustin SA (2011) Standardisation and reporting for nucleic acid quantifi cation. Accredit Qual Assur 16:399–405
    • (2011) Accredit Qual Assur , vol.16 , pp. 399-405
    • Huggett, J.1    Bustin, S.A.2
  • 20
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalisation; strategies and considerations
    • Huggett J, Dheda K, Bustin S et al (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6:279–284
    • (2005) Genes Immun , vol.6 , pp. 279-284
    • Huggett, J.1    Dheda, K.2    Bustin, S.3
  • 21
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    • Tricarico C, Pinzani P, Bianchi S et al (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309:293–300
    • (2002) Anal Biochem , vol.309 , pp. 293-300
    • Tricarico, C.1    Pinzani, P.2    Bianchi, S.3
  • 22
    • 27644563899 scopus 로고    scopus 로고
    • The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
    • Dheda K, Huggett JF, Chang JS et al (2005) The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344: 141–143
    • (2005) Anal Biochem , vol.344 , pp. 141-143
    • Dheda, K.1    Huggett, J.F.2    Chang, J.S.3
  • 23
    • 26844512816 scopus 로고    scopus 로고
    • Real-time reverse transcription PCR (QRT-PCR) and its potential use in clinical diagnosis
    • Bustin SA, Mueller R (2005) Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin Sci (Lond) 109: 365–379
    • (2005) Clin Sci (Lond) , vol.109 , pp. 365-379
    • Bustin, S.A.1    Mueller, R.2
  • 24
    • 33644914709 scopus 로고    scopus 로고
    • The real-time polymerase chain reaction
    • Kubista M, Andrade JM, Bengtsson M et al (2006) The real-time polymerase chain reaction. Mol Aspects Med 27:95–125
    • (2006) Mol Aspects Med , vol.27 , pp. 95-125
    • Kubista, M.1    Rade, J.M.2    Bengtsson, M.3
  • 25
    • 37549052481 scopus 로고    scopus 로고
    • Detection limits of several commercial reverse transcriptase enzymes: Impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR
    • Levesque-Sergerie JP, Duquette M, Thibault C et al (2007) Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR. BMC Mol Biol 8:93
    • (2007) BMC Mol Biol , vol.8 , pp. 93
    • Levesque-Sergerie, J.P.1    Duquette, M.2    Thibault, C.3
  • 26
    • 84887077614 scopus 로고    scopus 로고
    • The need for transparency and good practices in the qPCR literature
    • Bustin SA, Benes V, Garson J et al (2013) The need for transparency and good practices in the qPCR literature. Nat Methods 10: 1063–1067
    • (2013) Nat Methods , vol.10 , pp. 1063-1067
    • Bustin, S.A.1    Benes, V.2    Garson, J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.