Fluorescence assays for monitoring RNA-ligand interactions and riboswitch-targeted drug discovery screening

Methods in Enzymology

Volumn 550, Issue , 2015, Pages 363-383

  Liu, J ^a   Zeng, C ^a   Zhou, S ^a   Means, J A ^a   Hines, J V ^a  

^a OHIO UNIVERSITY   (United States)

Author keywords

Antiterminator; Fluorescence anisotropy; Fluorescence monitored thermal denaturation; Noncoding; RNA drug discovery; RNA ligand binding; Screening cascade; Steady state fluorescence; T box riboswitch

Indexed keywords

LIGAND; RNA; UNTRANSLATED RNA; RIBOSWITCH;

ARTICLE; DRUG BINDING; DRUG BINDING SITE; DRUG DETERMINATION; DRUG DEVELOPMENT; DRUG DISTRIBUTION; DRUG SCREENING; DRUG TARGETING; ENZYMOLOGY; FLUORESCENCE ANALYSIS; FLUORESCENCE MONITORED THERMAL DENATURATION ASSAY; HIGH THROUGHPUT SCREENING; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RIBOSWITCH; RNA BINDING; STEADY STATE; FLUORESCENCE POLARIZATION; GENETICS;

DRUG DISCOVERY; FLUORESCENCE POLARIZATION; RIBOSWITCH;

EID: 84921325255     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2014.10.040     Document Type: Chapter

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