Ginsenoside F2 possesses anti-obesity activity via binding with PPARγ and inhibiting adipocyte differentiation in the 3T3-L1 cell line

Journal of Enzyme Inhibition and Medicinal Chemistry

Volumn 30, Issue 1, 2015, Pages 9-14

  Siraj, Fayeza Md ^a   SathishKumar, Natarajan ^a   Kim, Yeon Ju ^a   Kim, Se Young ^a   Yang, Deok Chun ^a  

^a Kyung Hee University   (South Korea)

Author keywords

Adipogenesis; Ginsenoside F2; Molecular interaction; Obesity; Panax ginseng; PPAR

Indexed keywords

ANTIOBESITY AGENT; GINSENOSIDE; GINSENOSIDE CK; GINSENOSIDE F2; GINSENOSIDE RE; GINSENOSIDE RG 3; PERILIPIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; UNCLASSIFIED DRUG; CARRIER PROTEIN; PERILIPIN 1; PHOSPHOPROTEIN; PLANT EXTRACT; PROTEIN BINDING;

3T3 CELL LINE; ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ANTIOBESITY ACTIVITY; ARTICLE; BINDING AFFINITY; CELL DIFFERENTIATION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; DRUG ACTIVITY; DRUG PROTEIN BINDING; EMBRYO; GENE EXPRESSION; GLUCOSE TRANSPORT; HYDROGEN BOND; LIPID STORAGE; MOLECULAR DOCKING; MOLECULAR INTERACTION; MOUSE; NONHUMAN; OBESITY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; ANTAGONISTS AND INHIBITORS; BINDING SITE; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIPID METABOLISM; METABOLISM; PANAX;

PANAX GINSENG;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; ANIMALS; ANTI-OBESITY AGENTS; BINDING SITES; CARRIER PROTEINS; CELL DIFFERENTIATION; GENE EXPRESSION; GINSENOSIDES; HUMANS; LIPID METABOLISM; MICE; MOLECULAR DOCKING SIMULATION; PANAX; PHOSPHOPROTEINS; PLANT EXTRACTS; PPAR GAMMA; PROTEIN BINDING;

EID: 84921310261     PISSN: 14756366     EISSN: 14756374     Source Type: Journal    
DOI: 10.3109/14756366.2013.871006     Document Type: Article

References (29)

1. Yun JW. Possible anti-obesity therapeutics from nature - a review. Phytochemistry 2010;71:1625-41.
2. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 1999;58: 1685-93.
3. Gillis CN. Panax ginseng pharmacology: a nitric oxide link? Biochem Pharmacol 1997;54:1-8.
4. Wenbin S, Ying Y, Boren J, et al. Ginsenoside Rb1 promotes adipogenesis in 3T3-L1 cells by enhancing PPARγ2 and C/EBPα gene expression. Life Sci 2007;80:618-25.
5. Gu W, Kim KA, Kim DH. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm Bull 2013;36:102-7.
6. Hwang JT, Lee MS, Sung MJ, et al. Antiobesity effect of ginsenoside Rg3 involves the AMPK and PPAR-γ signal pathways. Phytother Res 2009;23:262-6.
7. Kim MS, Lee MS, Kim SH, et al. Anti-obesity Effects of Ginsenoside Rd via AMPK and PPAR Gamma. Korean J Biotech Bioeng 2007;22:341-4.
8. Hwang JT, Kim SH, Lee MS, et al. Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte. Biochem Biophys Res Commun 2007;364:1002-8.
9. Ko SR, Suzuki Y, Suzuki K, et al. Marked production of ginsenosides Rd, F2, Rg3, and compound K by enzymatic method. Chem Pharm Bull 2007;55:1522-7.
10. Shin JY, Lee JM, Shin HS, et al. Anti-cancer effect of ginsenoside F2 against glioblastoma multiforme in xenograft model in SD rats. J Ginseng Res 2012;36:86-92.
11. Mai TT, Moona JY, Song YW, et al. Ginsenoside F2 induces apoptosis accompanied by protective autophagy in breast cancer stem cells. Cancer Lett 2012;321:144-53.
12. Ma DL, Chana DSH, Leung CH. Molecular docking for virtual screening of natural product databases. Chem Sci 2011;2:1656-65.
13. Sohn YS, Lee Y, Park C, et al. Pharmacophore identification for peroxisome proliferator-activated receptor gamma agonists. Bull Korean Chem Soc 2011;32:201-7.
14. Berman HM, Westbrook J, Feng Z, et al. The protein data bank. Nucleic Acids Res 2000;28:235-42.
15. Goodsell DS, Morris GM, Olson AJ. Automated docking of flexible ligands: applications of AutoDock. J Mol Recognit 1996;9:1-5.
16. Jones G, Willett P, Glen RC, et al. Development and validation of a genetic algorithm for flexible docking. J Mol Biol 1997;267:727-48.
17. Rarey M, Kramer B, Lengauer T, Klebe G. A fast flexible docking method using an incremental construction algorithm. J Mol Biol 1996;261:470-89.
18. Morris GM, Goodsell DS, Halliday RS. Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function. J Comput Chem 1998;19:1639-62.
19. Park H, Lee J, Lee, S. Critical assessment of the automated AutoDock as a new docking tool for virtual screening. Prot Struct Funct Bioinform 2006;65:549-54.
20. Bursulaya BD, Totrov M, Abagyan R, Brooks III CL. Comparative study of several algorithms for flexible ligand docking. J Computer- Aided Mol Des 2003;17:755-63.
21. Sathishkumar N, Sathiyamoorthy S, Ramya M, et al. Molecular docking studies of anti-apoptotic BCL-2, BCL-XL, and MCL-1 proteins with ginsenosides from Panax ginseng. J Enzyme Inhibit Med Chem 2012;27:685-92.
22. Lagunin A, Stepanchikova A, Filimonov D, Poroikov V. PASS: prediction of activity spectra for biological active substances. Bioinform Appl Notes 2000;16:747-8.
23. Lagunin A, Filimonov D, Poroikov V. Multi-targeted natural products evaluation based on biological activity prediction with PASS. Curr Pharm Des 2010;16:1703-17.
24. Farmer SR. Regulation of PPARγ activity during adipogenesis. Int J Obes 2005;29:S13-16.
25. Park D, Yoon M. Compound K, a novel ginsenoside metabolite, inhibits adipocyte differentiation in 3T3-L1 cells: involvement of angiogenesis and MMPs. Biochem Biophys Res Commun 2012;422:263-7.
26. Lee OH, Lee HH, Kim JH, Lee BY. Effect of ginsenosides Rg3 and Re on glucose transport in mature 3T3-L1 adipocytes. Phytother Res 2011;25:768-73.
27. Kovsan J, Ben-Romano R, Souza SC, et al. Regulation of adipocyte lipolysis by degradation of the perilipin protein. J Biol Chem 2007;282:21704-11.
28. Blanchette-Mackie EJ, Dwyer NK, Barber LT, et al. Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes. J Lipid Res 1995;36:1211-26.
29. Rosen ED, Spiegelman BM. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol 2000;16:145-71.