Quantitative transporter proteomics by liquid chromatography with tandem mass spectrometry: Addressing methodologic issues of plasma membrane isolation and expression-activity relationship

Drug Metabolism and Disposition

Volumn 43, Issue 2, 2015, Pages 284-288

  Kumar, Vineet ^a   Prasad, Bhagwat ^a   Patilea, Gabriela ^a   Gupta, Anshul ^b   Salphati, Laurent ^d   Evers, Raymond ^c   Hop, Cornelis E C A ^d   Unadkat, Jashvant D ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b ASTRAZENECA   (United Kingdom)

^c MERCK RESEARCH LABORATORIES   (United States)

^d GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); BREAST CANCER RESISTANCE PROTEIN; ORGANIC ANION TRANSPORTER B; SHORT HAIRPIN RNA; SOLUTE CARRIER ORGANIC ANION TRANSPORTER 1B1; ABC TRANSPORTER; ABCG2 PROTEIN, HUMAN; BENZIMIDAZOLE DERIVATIVE; ESTRADIOL; HOE 33342; ORGANIC ANION TRANSPORTER; RECOMBINANT PROTEIN; SLCO1B1 PROTEIN, HUMAN; TUMOR PROTEIN;

ADULT; ANIMAL CELL; ARTICLE; CELL CLONE; CELL MEMBRANE; CONTROLLED STUDY; EMBRYO; ENZYME LOCALIZATION; HUMAN; HUMAN CELL; HUMAN TISSUE; LIQUID CHROMATOGRAPHY; LIVER; NONHUMAN; PROTEIN EXPRESSION; PROTEOMICS; TANDEM MASS SPECTROMETRY; TRANSPORT KINETICS; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOLOGICAL MODEL; CELL FRACTIONATION; CHEMISTRY; CHO CELL LINE; COMPARATIVE STUDY; CRICETULUS; DOG; DRUG SCREENING; GENE EXPRESSION REGULATION; GENETICS; HEK293 CELL LINE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KIDNEY; MDCK CELL LINE; METABOLISM; RNA INTERFERENCE; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BENZIMIDAZOLES; BIOLOGICAL TRANSPORT; CELL FRACTIONATION; CELL MEMBRANE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONE CELLS; CRICETULUS; DOGS; DRUG EVALUATION, PRECLINICAL; ESTRADIOL; GENE EXPRESSION REGULATION; HEK293 CELLS; HUMANS; KIDNEY; LIVER; MADIN DARBY CANINE KIDNEY CELLS; MODELS, BIOLOGICAL; NEOPLASM PROTEINS; ORGANIC ANION TRANSPORTERS; RECOMBINANT PROTEINS; RNA INTERFERENCE; TANDEM MASS SPECTROMETRY;

EID: 84921024813     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.114.061614     Document Type: Article

References (14)

1. Elia G (2012) Cell surface protein biotinylation for SDS-PAGE analysis. Methods Mol Biol 869: 361-372.
2. Gui C, Miao Y, Thompson L, Wahlgren B, Mock M, Stieger B, and Hagenbuch B (2008) Effect of pregnane X receptor ligands on transport mediated by human OATP1B1 and OATP1B3. Eur J Pharmacol 584:57-65.
3. Kawakami H, Ohtsuki S, Kamiie J, Suzuki T, Abe T, and Terasaki T (2011) Simultaneous absolute quantification of 11 cytochrome P450 isoforms in human liver microsomes by liquid chromatography tandem mass spectrometry with in silico target peptide selection. J Pharm Sci 100:341-352.
4. Kim M, Turnquist H, Jackson J, Sgagias M, Yan Y, Gong M, Dean M, Sharp JG, and Cowan K (2002) The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. Clin Cancer Res 8: 22-28.
5. Kopplow K, Letschert K, König J, Walter B, and Keppler D (2005) Human hepatobiliary transport of organic anions analyzed by quadruple-transfected cells. Mol Pharmacol 68: 1031-1038.
6. Padilla-Benavides T, Roldán ML, Larre I, Flores-Benitez D, Villegas-Sepúlveda N, Contreras RG, Cereijido M, and Shoshani L (2010) The polarized distribution of Na+, K+-ATPase: role of the interaction between beta subunits. Mol Biol Cell 21:2217-2225.
7. Prasad B and Unadkat JD (2014) Optimized approaches for quantification of drug transporters in tissues and cells by MRM proteomics. AAPS J 16:634-648.
8. Prasad B, Evers R, Gupta A, Hop CE, Salphati L, Shukla S, Ambudkar SV, and Unadkat JD (2014) Interindividual variability in hepatic organic anion-transporting polypeptides and P-glycoprotein (ABCB1) protein expression: quantification by liquid chromatography tandem mass spectroscopy and influence of genotype, age, and sex. Drug Metab Dispos 42: 78-88.
9. Prasad B, Lai Y, Lin Y, and Unadkat JD (2013) Interindividual variability in the hepatic expression of the human breast cancer resistance protein (BCRP/ABCG2): effect of age, sex, and genotype. J Pharm Sci 102:787-793.
10. Qiu X, Bi YA, Balogh LM, and Lai Y (2013) Absolute measurement of species differences in sodium taurocholate cotransporting polypeptide (NTCP/Ntcp) and its modulation in cultured hepatocytes. J Pharm Sci 102:3252-3263.
11. Schaefer O, Ohtsuki S, Kawakami H, Inoue T, Liehner S, Saito A, Sakamoto A, Ishiguro N, Matsumaru T, Terasaki T, and Ebner T (2012) Absolute quantification and differential expression of drug transporters, cytochrome P450 enzymes, and UDP-glucuronosyltransferases in cultured primary human hepatocytes. Drug Metab Dispos 40:93-103.
12. Suski JM, Lebiedzinska M, Wojtala A, Duszynski J, Giorgi C, Pinton P, and Wieckowski MR (2014) Isolation of plasma membrane-associated membranes from rat liver. Nat Protoc 9: 312-322.
13. Xia CQ, Liu N, Yang D, Miwa G, and Gan LS (2005) Expression, localization, and functional characteristics of breast cancer resistance protein in Caco-2 cells. Drug Metab Dispos 33: 637-643.
14. Yamazaki M, Li B, Louie SW, Pudvah NT, Stocco R, Wong W, Abramovitz M, Demartis A, Laufer R, and Hochman JH, et al. (2005) Effects of fibrates on human organic aniontransporting polypeptide 1B1-, multidrug resistance protein 2-and P-glycoprotein-mediated transport. Xenobiotica 35:737-753.