Purslane suppresses osteoclast differentiation and bone resorbing activity via inhibition of Akt/GSK3β-c-Fos-NFATc1 signaling in vitro and prevents lipopolysaccharide-induced bone loss in vivo

Biological and Pharmaceutical Bulletin

Volumn 38, Issue 1, 2015, Pages 66-74

  Kim, Ju Young ^a   Oh, Hyun Mee ^b   Kwak, Sung Chul ^c   Cheon, Yoon Hee ^a   Lee, Myeung Su ^a   Rho, Mun Chual ^b   Oh, Jaemin ^a  

^a Wonkwang University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c Korea Institute of Science and Technology   (South Korea)

Author keywords

Bone loss; Natural medicine; Osteoclast; Osteoporosis; Purslane (Portulaca oleracea)

Indexed keywords

ACID PHOSPHATASE TARTRATE RESISTANT ISOENZYME; F ACTIN; GLYCOGEN SYNTHASE KINASE 3BETA; LIPOPOLYSACCHARIDE; OSTEOCLAST DIFFERENTIATION FACTOR; PORTULACA OLERACEA EXTRACT; PROTEIN C FOS; PROTEIN KINASE B; TRANSCRIPTION FACTOR NFAT; GLYCOGEN SYNTHASE KINASE 3; GLYCOGEN SYNTHASE KINASE 3 BETA; PLANT EXTRACT;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BONE MASS; BONE STRUCTURE; CELL ASSAY; CELL DIFFERENTIATION; CELL FUSION; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; DRUG MECHANISM; GENE CONTROL; HISTOPATHOLOGY; IN VITRO STUDY; IN VIVO STUDY; INFLAMMATION; MACROPHAGE FUNCTION; MALE; MICRO-COMPUTED TOMOGRAPHY; MOUSE; MOUSE MODEL; NONHUMAN; OSTEOCLAST; OSTEOCLASTOGENESIS; OSTEOLYSIS; PORTULACACEAE; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; ANIMAL; ANTAGONISTS AND INHIBITORS; BONE MARROW CELL; CHEMICALLY INDUCED; CYTOLOGY; DRUG EFFECTS; INSTITUTE FOR CANCER RESEARCH MOUSE; METABOLISM; PORTULACA; PREVENTION AND CONTROL;

ANIMALS; BONE MARROW CELLS; BONE RESORPTION; CELL DIFFERENTIATION; GLYCOGEN SYNTHASE KINASE 3; LIPOPOLYSACCHARIDES; MALE; MICE, INBRED ICR; NFATC TRANSCRIPTION FACTORS; OSTEOCLASTS; PLANT EXTRACTS; PORTULACA; PROTO-ONCOGENE PROTEINS C-AKT; PROTO-ONCOGENE PROTEINS C-FOS; RANK LIGAND; SIGNAL TRANSDUCTION;

EID: 84920531638     PISSN: 09186158     EISSN: 13475215     Source Type: Journal    
DOI: 10.1248/bpb.b14-00567     Document Type: Article

References (26)

1. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature, 423, 337-342 (2003).
2. Harada S, Rodan GA. Control of osteoblast function and regulation of bone mass. Nature, 423, 349-355 (2003).
3. Lacey DL, Timms E, Tan HL, Kelley MJ, Dunstan CR, Burgess T, Elliott R, Colombero A, Elliott G, Scully S, Hsu H, Sullivan J, Hawkins N, Davy E, Capparelli C, Eli A, Qian YX, Kaufman S, Sarosi I, Shalhoub V, Senaldi G, Guo J, Delaney J, Boyle WJ. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell, 93, 165-176 (1998).
4. Jurdic P, Saltel F, Chabadel A, Destaing O. Podosome and sealing zone: specificity of the osteoclast model. Eur. J. Cell Biol., 85, 195-202 (2006).
5. Kong YY, Yoshida H, Sarosi I, Tan HL, Timms E, Capparelli C, Morony S, Oliveira-dos-Santos AJ, Van G, Itie A, Khoo W, Wakeham A, Dunstan CR, Lacey DL, Mak TW, Boyle WJ, Penninger JM. OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature, 397, 315-323 (1999).
6. Takayanagi H. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat. Rev. Immunol., 7, 292-304 (2007).
7. Grigoriadis AE, Wang ZQ, Cecchini MG, Hofstetter W, Felix R, Fleisch HA, Wagner EF. c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling. Science, 266, 443-448 (1994).
8. Matsuo K, Galson DL, Zhao C, Peng L, Laplace C, Wang KZ, Bachler MA, Amano H, Aburatani H, Ishikawa H, Wagner EF. Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos. J. Biol. Chem., 279, 26475-26480 (2004).
9. Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner EF, Mak TW, Kodama T, Taniguchi T. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev. Cell, 3, 889-901 (2002).
10. Yamashita T, Yao Z, Li F, Zhang Q, Badell IR, Schwarz EM, Takeshita S, Wagner EF, Noda M, Matsuo K, Xing L, Boyce BF. NF-kappaB p50 and p52 regulate receptor activator of NF-kappaB ligand (RANKL) and tumor necrosis factor-induced osteoclast precursor differentiation by activating c-Fos and NFATc1. J. Biol. Chem., 282, 18245-18253 (2007).
11. Uddin MK, Juraimi AS, Hossain MS, Nahar MA, Ali ME, Rahman MM. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. Scientific World Journal, 2014, 951019 (2014).
12. Liu L, Howe P, Zhou YF, Xu ZQ, Hocart C, Zhang R. Fatty acids and beta-carotene in australian purslane (Portulaca oleracea) varieties. J. Chromatogr. A, 893, 207-213 (2000).
13. Xiang L, Xing D, Wang W, Wang R, Ding Y, Du L. Alkaloids from Portulaca oleracea L. Phytochemistry, 66, 2595-2601 (2005).
14. Chan K, Islam MW, Kamil M, Radhakrishnan R, Zakaria MN, Habibullah M, Attas A. The analgesic and anti-inflammatory effects of Portulaca oleracea L. ssp. Sativa (HAW.) CELAK. J. Ethnopharmacol., 73, 445-451 (2000).
15. Silva R, Carvalho IS. In vitro antioxidant activity, phenolic compounds and protective effect against DNA damage provided by leaves, stems and flowers of Portulaca oleracea (PURSLANE). Nat. Prod. Commun., 9, 45-50 (2014).
16. Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J. Ethnopharmacol., 88, 131-136 (2003).
17. Cao H, Zhu K, Qiu L, Li S, Niu H, Hao M, Yang S, Zhao Z, Lai Y, Anderson JL, Fan J, Im HJ, Chen D, Roodman GD, Xiao G. Critical role of AKT protein in myeloma-induced osteoclast formation and osteolysis. J. Biol. Chem., 288, 30399-30410 (2013).
18. Moon JB, Kim JH, Kim K, Youn BU, Ko A, Lee SY, Kim N. Akt induces osteoclast differentiation through regulating the GSK3beta/NFATc1 signaling cascade. J. Immunol., 188, 163-169 (2012).
19. Gingery A, Bradley E, Shaw A, Oursler MJ. Phosphatidylinositol 3-kinase coordinately activates the MEK/ERK and AKT/NF-kappaB pathways to maintain osteoclast survival. J. Cell. Biochem., 89, 165-179 (2003).
20. Wong BR, Besser D, Kim N, Arron JR, Vologodskaia M, Hanafusa H, Choi Y. TRANCE, a TNF family member, activates Akt/PKB through a signaling complex involving TRAF6 and c-Src. Mol. Cell, 4, 1041-1049 (1999).
21. Angel NZ, Walsh N, Forwood MR, Ostrowski MC, Cassady AI, Hume DA. Transgenic mice overexpressing tartrate-resistant acid phosphatase exhibit an increased rate of bone turnover. J. Bone Miner. Res., 15, 103-110 (2000).
22. Nemeth K, Schoppet M, Al-Fakhri N, Helas S, Jessberger R, Hofbauer LC, Goettsch C. The role of osteoclast-associated receptor in osteoimmunology. J. Immunol., 186, 13-18 (2011).
23. Delaissé J-M, Andersen TL, Engsig MT, Henriksen K, Troen T, Blavier L. Matrix metalloproteinases (MMP) and cathepsin K contribute differently to osteoclastic activities. Microsc. Res. Tech., 61, 504-513 (2003).
24. Kim K, Lee SH, Ha Kim J, Choi Y, Kim N. NFATc1 induces osteoclast fusion via up-regulation of Atp6v0d2 and the dendritic cellspecific transmembrane protein (DC-STAMP). Mol. Endocrinol., 22, 176-185 (2008).
25. Yang M, Birnbaum MJ, MacKay CA, Mason-Savas A, Thompson B, Odgren PR. Osteoclast stimulatory transmembrane protein (OC-STAMP), a novel protein induced by RANKL that promotes osteoclast differentiation. J. Cell. Physiol., 215, 497-505 (2008).
26. Nakamura I, Duong LT, Rodan SB, Rodan GA. Involvement of alpha (v) beta3 integrins in osteoclast function. J. Bone Miner. Metab., 25, 337-344 (2007).