Cryopreservation of MHC multimers: Recommendations for quality assurance in detection of antigen specific T cells

Cytometry Part A

Volumn 87, Issue 1, 2015, Pages 37-48

  Hadrup, Sine Reker ^a   Maurer, Dominik ^b   Laske, Karoline ^c   Frøsig, Thomas Mørch ^a   Andersen, Sofie Ramskov ^a   Britten, Cedrik M ^d   van der Burg, Sjoerd H ^e   Walter, Steffen ^b   Gouttefangeas, Cécile ^c  

^a GENTOFTE UNIVERSITY HOSPITAL   (Denmark)

^b IMMATICS BIOTECHNOLOGIES GMBH   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

^d UNIVERSITY MEDICAL CENTER MAINZ   (Germany)

^e LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cryopreservation; Cryoprotectant; Glycerol in T cell staining; MHC multimer; Quality assurance; Recommendations for MHC multimer storage

Indexed keywords

CRYOPROTECTIVE AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FLUORESCENT DYE; HLA ANTIGEN CLASS 1; PEPTIDE; QUANTUM DOT;

CHEMISTRY; CRYOPRESERVATION; FLOW CYTOMETRY; HUMAN; IMMUNOLOGY; PATHOLOGY; PROTEIN MULTIMERIZATION; QUALITY CONTROL; REPRODUCIBILITY; STAINING; STANDARDS; T LYMPHOCYTE;

CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; FLOW CYTOMETRY; FLUORESCENT DYES; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; INDICATORS AND REAGENTS; PEPTIDES; PROTEIN MULTIMERIZATION; QUALITY CONTROL; QUANTUM DOTS; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING; T-LYMPHOCYTES;

EID: 84919873655     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22575     Document Type: Article

References (34)

1. Altman JD, Moss PA, Goulder PJ, Barouch DH, McHeyzer-Williams MG, Bell JI, McMichael AJ, Davis MM. Phenotypic analysis of antigen-specific T lymphocytes. Science 1996;274:94-96.
2. Davis MM, Altman JD, Newell EW. Interrogating the repertoire: broadening the scope of peptide-MHC multimer analysis. Nat Rev Immunol 2011;11:551-558.
3. Appay V, Dunbar PR, Callan M, Klenerman P, Gillespie GM, Papagno L, Ogg GS, King A, Lechner F, Spina CA, et al. Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections. Nat Med 2002;8:379-385.
4. Tan LC, Gudgeon N, Annels NE, Hansasuta P, O'Callaghan CA, Rowland-Jones S, McMichael AJ, Rickinson AB, Callan MF. A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers. J Immunol 1999;162:1827-1835.
5. Khan N, Cobbold M, Cummerson J, Moss PAH. Persistent viral infection in humans can drive high frequency low-affinity T-cell expansions. Immunology 2010;131:537-548.
6. Walter S, Weinschenk T, Stenzl A, Zdrojowy R, Pluzanska A, Szczylik C, Staehler M, Brugger W, Dietrich P-Y, Mendrzyk R, et al. Multipeptide immune response to cancer vaccine IMA901 after single-dose cyclophosphamide associates with longer patient survival. Nat Med 2012;18:1254-1261.
7. Van Rooij N, van Buuren MM, Philips D, Velds A, Toebes M, Heemskerk B, van Dijk LJA, Behjati S, Hilkmann H, El Atmioui D, et al. Tumor exome analysis reveals neoantigen-specific T-cell reactivity in an ipilimumab-responsive melanoma. J Clin Oncol 2013;31:e439-e442.
8. Andersen RS, Thrue CA, Junker N, Lyngaa R, Donia M, Ellebæk E, Svane IM, Schumacher TN, Thor Straten P, Hadrup SR. Dissection of T-cell antigen specificity in human melanoma. Cancer Res 2012;72:1642-1650.
9. Perfetto SP, Ambrozak D, Nguyen R, Chattopadhyay PK, Roederer M. Quality assurance for polychromatic flow cytometry using a suite of calibration beads. Nat Protoc 2012;7:2067-2079.
10. Chattopadhyay PK, Melenhorst JJ, Ladell K, Gostick E, Scheinberg P, Barrett AJ, Wooldridge L, Roederer M, Sewell AK, Price DA. Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers. Cytometry A 2008;73A:1001-1009.
11. Maecker HT, McCoy JP, Nussenblatt R. Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol 2012;12:191-200.
12. Britten CM, Gouttefangeas C, Welters MJP, Pawelec G, Koch S, Ottensmeier C, Mander A, Walter S, Paschen A, Müller-Berghaus J, et al. The CIMT-monitoring panel: a two-step approach to harmonize the enumeration of antigen-specific CD8+ T lymphocytes by structural and functional assays. Cancer Immunol Immunother 2008;57:289-302.
13. Britten CM, Janetzki S, Ben-Porat L, Clay TM, Kalos M, Maecker H, Odunsi K, Pride M, Old L, Hoos A, et al. Harmonization guidelines for HLA-peptide multimer assays derived from results of a large scale international proficiency panel of the Cancer Vaccine Consortium. Cancer Immunol Immunother 2009;58:1701-1713.
14. Van der Burg SH, Kalos M, Gouttefangeas C, Janetzki S, Ottensmeier C, Welters MJP, Romero P, Britten CM, Hoos A. Harmonization of immune biomarker assays for clinical studies. Sci Transl Med 2011;3:108-144.
15. Attig S, Price L, Janetzki S, Kalos M, Pride M, McNeil L, Clay T, Yuan J, Odunsi K, Hoos A, et al. A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols. J Transl Med 2011;9:108.
16. Hadrup SR, Bakker AH, Shu CJ, Andersen RS, van VJ, Hombrink P, Castermans E, Thor SP, Blank C, Haanen JB, et al. Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers. Nat Methods 2009;6:520-526.
17. Newell EW, Klein LO, Yu W, Davis MM. Simultaneous detection of many T-cell specificities using combinatorial tetramer staining. Nat Methods 2009;6:497-499.
18. Andersen RS, Kvistborg P, Moerch TF, Pedersen NW, Lyngaa R, Bakker AH, Shu CJ, thor Straten P, Schumacher TN, Hadrup SR. Parallel detection of antigen-specific T-cell responses by combinatorial encoding of MHC multimers. Nat Protoc 2012;7:891-902.
19. Schumacher TN, Heemels MT, Neefjes JJ, Kast WM, Melief CJ, Ploegh HL. Direct binding of peptide to empty MHC class I molecules on intact cells and in vitro. Cell 1990;62:563-567.
20. Emerson TE. Unique features of albumin: A brief review. Crit Care Med 1989;17:690-694.
21. Fuller BJ. Cryoprotectants: The essential antifreezes to protect life in the frozen state. Cryo Letters 2004;25:375-88.
22. Haanen JB, Wolkers MC, Kruisbeek AM, Schumacher TN. Selective expansion of cross-reactive CD8(+) memory T cells by viral variants. J Exp Med 1999;190:1319-1328.
23. Haanen JB, van Oijen MG, Tirion F, Oomen LC, Kruisbeek AM, Vyth-Dreese FA, Schumacher TN. In situ detection of virus- and tumor-specific T-cell immunity. Nat Med 2000;6:1056-1060.
24. Britten CM, Janetzki S, Butterfield LH, Ferrari G, Gouttefangeas C, Huber C, Kalos M, Levitsky HI, Maecker HT, Melief CJM, et al. T cell assays and MIATA: The essential minimum for maximum impact. Immunity 2012;37:1-2.
25. Ellebaek E, Iversen TZ, Junker N, Donia M, Engell-Noerregaard L, Met O, Hölmich LR, Andersen RS, Hadrup SR, Andersen MH, et al. Adoptive cell therapy with autologous tumor infiltrating lymphocytes and low-dose Interleukin-2 in metastatic melanoma patients. J Transl Med 2012;10:169.
26. Weinschenk T, Gouttefangeas C, Schirle M, Obermayr F, Walter S, Schoor O, Kurek R, Loeser W, Bichler K, Wernet D, et al. Integrated functional genomics approach for the design of patient-individual antitumor vaccines integrated functional genomics approach for the design of patient-individual. 2002;62:5818-5827.
27. Walter S, Bioley G, Bühring H-J, Koch S, Wernet D, Zippelius A, Pawelec G, Romero P, Stevanović S, Rammensee H-G, et al. High frequencies of functionally impaired cytokeratin 18-specific CD8+ T cells in healthy HLA-A2+ donors. Eur J Immunol 2005;35:2876-2885.
28. Hadrup SR, Toebes M, Rodenko B, Bakker AH, Egan DA, Ovaa H, Schumacher TNM. High-throughput T-cell epitope discovery through MHC peptide exchange. Methods Mol Biol 2009;524:383-405.
29. Maecker HT, Frey T, Nomura LE, Trotter J. Selecting fluorochrome conjugates for maximum sensitivity. Cytometry A 2004;62A:169-173.
30. Fishman AI, Alexander B, Eshghi M, Choudhury M, Konno S. Nephrotoxin-induced renal cell injury involving biochemical alterations and its prevention with antioxidant. J Clin Med Res 2012;4:95-101.
31. Fahning ML, Garcia MA. Status of cryopreservation of embryos from domestic animals. Cryobiology 1992;29:1-18.
32. Nguyen R, Perfetto S, Mahnke YD, Chattopadhyay P, Roederer M. Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A 2013;83A:306-315.
33. Toebes M, Coccoris M, Bins AD, Rodenko B, Gomez R, Nieuwkoop NJ, van de Kasteele W, Rimmelzwaan G, Haanen JB, Schumacher TN. Design and use of conditional MHC class I ligands. Nat.Med. 2006;12:246-251.
34. Bakke M, Kajiyama N. Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification. Appl. Biochem. Biotechnol. 2004;112:123-31.