Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design

Cytometry Part A

Volumn 83 A, Issue 3, 2013, Pages 306-315

  Nguyen, Richard ^a   Perfetto, Stephen ^a   Mahnke, Yolanda D ^a   Chattopadhyay, Pratip ^a   Roederer, Mario ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Compensation; Immunophenotyping; Quality control; Sensitivity

Indexed keywords

FLUORESCENT DYE;

ARTICLE; ARTIFACT; COLOR; EQUIPMENT; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; MONONUCLEAR CELL; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; STATISTICS;

ARTIFACTS; COLOR; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; HUMANS; LEUKOCYTES, MONONUCLEAR; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY;

EID: 84874116408     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22251     Document Type: Article

References (7)

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3. Baumgarth N, Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000; 243: 77-97.
4. Mahnke YD, Roederer M. Optimizing a multicolor immunophenotyping assay. Clin Lab Med 2007; 27: 469-485, v.
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6. Mahnke YD, Beddall MH, Roederer M. OMIP-015: Human regulatory and activated T-cells without intracellular staining. Cytometry A 2013; 83A: 179-181.
7. Roederer M. Compensation is not dependent on signal intensity or on number of parameters. Cytometry 2001; 46: 357-359.