Biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli by utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase

Applied and Environmental Microbiology

Volumn 80, Issue 21, 2014, Pages 6828-6836

  Miki, Yuta ^a,b   Asano, Yasuhisa ^a,b  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; BIOSYNTHESIS; CYANIDES; ENCODING (SYMBOLS); ENZYMES; GENES;

ALDOXIME DEHYDRATASE; ALDOXIME-NITRILE PATHWAY; ARABIDOPSIS THALIANA; BIOSYNTHETIC PATHWAY; N-TERMINAL TRUNCATION; OPTIMIZED CONDITIONS; PHARMACEUTICAL INDUSTRY; PLANT CYTOCHROME P450;

ESCHERICHIA COLI;

CONCENTRATION (COMPOSITION); CYANIDE; CYTOCHROME; ENZYME ACTIVITY; GENE EXPRESSION; METABOLITE; MICROBIAL ACTIVITY; PLANT COMMUNITY; REACTION KINETICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ACETONITRILE DERIVATIVE; ALDOXIME DEHYDRATASE; BACTERIAL PROTEIN; BENZYL CYANIDE; CYTOCHROME P450; ESCHERICHIA COLI PROTEIN; GROE PROTEIN, E COLI; HEAT SHOCK PROTEIN; HYDROLYASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; VEGETABLE PROTEIN;

ARABIDOPSIS; BACILLUS; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLIC ENGINEERING; METABOLISM;

ACETONITRILES; ARABIDOPSIS; BACILLUS; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEAT-SHOCK PROTEINS; HYDRO-LYASES; METABOLIC ENGINEERING; NADPH-FERRIHEMOPROTEIN REDUCTASE; PLANT PROTEINS; RECOMBINANT PROTEINS;

EID: 84914145225     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01623-14     Document Type: Article

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