Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES

Biochemical and Biophysical Research Communications

Volumn 323, Issue 2, 2004, Pages 505-511

  Uchida, Eriko ^a   Kagawa, Norio ^b   Sakaki, Toshiyuki ^c   Urushino, Naoko ^a   Sawada, Natsumi ^d   Kamakura, Masaki ^c   Ohta, Miho ^e   Kato, Shigeaki ^f   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^d UNIVERSITY OF SHIZUOKA   (Japan)

^e Koshien Junior College   (Japan)

^f UNIVERSITY OF TOKYO   (Japan)

Author keywords

CYP27B1; GroEL ES; Molecular chaperonin; Vitamin D 1 hydroxylation; Vitamin D 25 hydroxylation

Indexed keywords

ALFACALCIDOL; CALCIFEDIOL; CHAPERONIN; COLECALCIFEROL; CYTOCHROME; CYTOCHROME P450; CYTOCHROME P450 105A1; CYTOCHROME P450 27B1; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROXYLATION; MEASUREMENT; MOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STREPTOMYCES;

25-HYDROXYVITAMIN D3 1-ALPHA-HYDROXYLASE; ANIMALS; CHAPERONINS; CLONING, MOLECULAR; ENZYME ACTIVATION; ESCHERICHIA COLI; GENETIC ENHANCEMENT; GROEL PROTEIN; GROES PROTEIN; MICE; MOLECULAR WEIGHT; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; STREPTOMYCES;

EID: 4544258603     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.110     Document Type: Article

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