Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments

Journal of Immunological Methods

Volumn 415, Issue , 2014, Pages 24-30

  Bailey, Lucas J ^a   Sheehy, Kimberly M ^a   Hoey, Robert J ^a   Schaefer, Zachary P ^a   Ura, Marcin ^a   Kossiakoff, Anthony A ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Affinity maturation; Affinity reagent; Antibody fragment purification; Immunoglobulin binding protein; Phage display; Protein G

Indexed keywords

ANTIBODY; BINDING PROTEIN; DIMER; IMMUNOGLOBULIN F(AB) FRAGMENT; PROTEIN G; PROTEIN G A1; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; IGG FC-BINDING PROTEIN, STREPTOCOCCUS; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; PEPTIDE LIBRARY; PROTEIN BINDING; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DIMERIZATION; MUTAGENESIS; NONHUMAN; PH; PHAGE DISPLAY; POINT MUTATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; STREPTOCOCCUS; AMINO ACID SEQUENCE; ANTIBODY AFFINITY; CHEMISTRY; GENETICS; IMMUNOLOGY; KINETICS; MOLECULAR GENETICS; MUTATION; PEPTIDE LIBRARY; PROCEDURES; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; BACTERIAL PROTEINS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

EID: 84912526074     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.10.003     Document Type: Article

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