Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

Analytical Biochemistry

Volumn 417, Issue 1, 2011, Pages 25-35

  Dyson, Michael R ^a   Zheng, Yong ^b   Zhang, Cunjie ^b   Colwill, Karen ^b   Pershad, Kritika ^c   Kay, Brian K ^c   Pawson, Tony ^b,d   McCafferty, John ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b MOUNT SINAI HOSPITAL   (Canada)

^c UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

Affinity maturation; Antibody phage display; Immunoprecipitation; Mass spectrometry; Protein protein interaction networks

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BINDERS; BINDING SITES; EPITOPES; GENES; MAPPING; MASS SPECTROMETRY; PHAGE DISPLAY;

AFFINITY MATURATION; AFFINITY THRESHOLDS; EPIDERMAL GROWTH FACTORS; HUMAN BREAST CANCER; IMMUNOPRECIPITATIONS; PHOSPHORYLATION SITES; PROTEIN-PROTEIN INTERACTION NETWORKS; SRC HOMOLOGY 2 DOMAINS;

ANTIBODIES;

CELL PROTEIN; CRK PROTEIN; EPITOPE; NCK1 PROTEIN; PROTEIN KINASE LCK; PROTEIN KINASE LYN; PROTEIN KINASE ZAP 70; PROTEIN SH2; PROTEIN TYROSINE PHOSPHATASE SHP 2; SHC1 PROTEIN; UNCLASSIFIED DRUG; VAV1 PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTOR; HYBRID PROTEIN; PEPTIDE LIBRARY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; BREAST EPITHELIUM; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; CELL STRAIN HEK293; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; KINETICS; METABOLISM; METHODOLOGY; PROTEIN MULTIMERIZATION; SIGNAL TRANSDUCTION; SRC HOMOLOGY DOMAIN; TUMOR CELL LINE;

ANTIBODY AFFINITY; CELL LINE, TUMOR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEK293 CELLS; HUMANS; IMMUNOPRECIPITATION; KINETICS; MASS SPECTROMETRY; PEPTIDE LIBRARY; PROTEIN INTERACTION MAPPING; PROTEIN MULTIMERIZATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; SINGLE-CHAIN ANTIBODIES; SRC HOMOLOGY DOMAINS;

EID: 80655145637     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.05.005     Document Type: Article

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