Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering

Journal of Surgical Research

Volumn 192, Issue 2, 2014, Pages 454-463

  Xing, Junchao ^a,b,c   Jin, Huiyong ^a,b,c,d   Hou, Tianyong ^a,b,c   Chang, Zhengqi ^a,b,c   Luo, Fei ^a,b,c   Wang, Pinpin ^a,b,c   Li, Zhiqiang ^a,b,c   Xie, Zhao ^a,b,c   Xu, Jianzhong ^a,b,c  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b Laboratory of Tissue Engineering in Chongqing City   (China)

^c Center of Regenerative and Reconstructive Engineering Technology   (China)

^d Military Hospital No 519   (China)

Author keywords

Bilateral bone defect; Mesenchymal stem cells; Mouse model; Tissue engineering

Indexed keywords

CD31 ANTIGEN; TITANIUM; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASIC RESEARCH; BONE DEFECT; BONE MARROW DERIVED MESENCHYMAL STEM CELL; BONE MATRIX; BONE PLATE; BONE RADIOGRAPHY; BONE TISSUE; BONE TRANSPLANTATION; COMPUTED TOMOGRAPHY SCANNER; CONTROLLED STUDY; CORTICAL BONE; ENDOTHELIAL PROGENITOR CELL; FEASIBILITY STUDY; FEMALE; FEMUR; FEMUR IMPLANT; FRACTURE FIXATION; FRACTURE HEALING; HISTOLOGY; IMMUNOFLUORESCENCE; LARGE SEGMENTAL BONE DEFECT; MESENCHYMAL STEM CELL TRANSPLANTATION; MICRO-COMPUTED TOMOGRAPHY; MOUSE; MOUSE MODEL; NONHUMAN; OSTEOSYNTHESIS; POSTOPERATIVE PERIOD; RADIOGRAPHY DEVICE; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; TISSUE ENGINEERING; TISSUE SCAFFOLD; ANIMAL; BONE DEVELOPMENT; CYTOLOGY; DISEASE MODEL; FEMORAL FRACTURES; GENETICS; INBRED MOUSE STRAIN; MALE; MESENCHYMAL STROMA CELL; PROCEDURES; PROSTHESES AND ORTHOSES; RADIOGRAPHY; TRANSGENIC MOUSE; WEIGHT BEARING;

ANIMALS; BONE PLATES; BONE TRANSPLANTATION; DISEASE MODELS, ANIMAL; FEMORAL FRACTURES; FEMUR; FRACTURE HEALING; GREEN FLUORESCENT PROTEINS; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; MICE, INBRED STRAINS; MICE, TRANSGENIC; OSTEOGENESIS; PROSTHESES AND IMPLANTS; REPRODUCIBILITY OF RESULTS; TISSUE ENGINEERING; WEIGHT-BEARING;

EID: 84912106510     PISSN: 00224804     EISSN: 10958673     Source Type: Journal    
DOI: 10.1016/j.jss.2014.05.037     Document Type: Article

References (35)

1. Gomes PS, Fernandes MH. Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies. Lab Anim 2011;45:14.
2. Horner EA, Kirkham J, Wood D, et al. Long bone defect models for tissue engineering applications: criteria for choice. Tissue engineering. Part B, Reviews 2010;16:263.
3. Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG. Animal models for implant biomaterial research in bone: a review. Eur cells & materials 2007;13:1.
4. Bensaid W, Oudina K, Viateau V, et al. De novo reconstruction of functional bone by tissue engineering in the metatarsal sheep model. Tissue engineering 2005;11:814.
5. Bruder SP, Kraus KH, Goldberg VM, Kadiyala S. The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects. The Journal of bone and joint surgery. American volume 1998;80:985.
6. Pek YS, Gao S, Arshad MS, Leck KJ, Ying JY. Porous collagenapatite nanocomposite foams as bone regeneration scaffolds. Biomaterials 2008;29:4300.
7. Petite H, Viateau V, Bensaid W, et al. Tissue-engineered bone regeneration. Nature biotechnology 2000;18:959.
8. Muschler GF, Raut VP, Patterson TE, Wenke JC, Hollinger JO. The design and use of animal models for translational research in bone tissue engineering and regenerative medicine. Tissue engineering. Part B, Reviews 2010;16:123.
9. Soleimani M, Nadri S. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nature protocols 2009;4:102.
10. Liu G, Li Y, Sun J, et al. In vitro and in vivo evaluation of osteogenesis of human umbilical cord blood-derived mesenchymal stem cells on partially demineralized bone matrix. Tissue engineering. Part A 2010;16:971.
11. Liu G, Sun J, Li Y, et al. Evaluation of partially demineralized osteoporotic cancellous bone matrix combined with human bone marrow stromal cells for tissue engineering: an in vitro and in vivo study. Calcified tissue international 2008;83:176.
12. Mauney JR, Jaquiery C, Volloch V, Heberer M, Martin I, Kaplan DL. In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering. Biomaterials 2005;26:3173.
13. Tortelli F, Tasso R, Loiacono F, Cancedda R. The development of tissue-engineered bone of different origin through endochondral and intramembranous ossification following the implantation of mesenchymal stem cells and osteoblasts in a murine model. Biomaterials 2010;31:242.
14. Zhou Y, Fan W, Prasadam I, Crawford R, Xiao Y. Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells. Journal of tissue engineering and regenerative medicine; 2012. http://dx.doi.org/10.1002/term.1619 [Epub ahead of print].
15. Nordgren A. Moral imagination in tissue engineering research on animal models. Biomaterials 2004;25:1723.
16. Hou T, Li Q, Luo F, et al. Controlled dynamization to enhance reconstruction capacity of tissue-engineered bone in healing critically sized bone defects: an in vivo study in goats. Tissue engineering. Part A 2010;16:201.
17. Kon E, Muraglia A, Corsi A, et al. Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones. Journal of biomedical materials research 2000;49:328.
18. Bruder SP, Kurth AA, Shea M, Hayes WC, Jaiswal N, Kadiyala S. Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells. Journal of orthopaedic research 1998;16:155.
19. Stubbs D, Deakin M, Chapman-Sheath P, et al. In vivo evaluation of resorbable bone graft substitutes in a rabbit tibial defect model. Biomaterials 2004;25:5037.
20. Srouji S, Ben-David D, Kohler T, Muller R, Zussman E, Livne E. A model for tissue engineering applications: femoral critical size defect in immunodeficient mice. Tissue engineering. Part C, Methods 2011;17:597.
21. Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clinical orthopaedics and related research 1986;205:299.
22. Reichert JC, Saifzadeh S, Wullschleger ME, et al. The challenge of establishing preclinicalmodels for segmental bone defect research. Biomaterials 2009;30:2149.
23. Holt J, Hertzberg B, Weinhold P, Storm W, Schoenfisch M, Dahners L. Decreasing bacterial colonization of external fixation pins through nitric oxide release coatings. Journal of orthopaedic trauma 2011;25:432.
24. Drosse I, Volkmer E, Seitz S, et al. Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue engineering. Part C, Methods 2008;14:79.
25. Zwingenberger S, Niederlohmann E, Vater C, et al. Establishment of a femoral critical-size bone defect model in immunodeficient mice. The Journal of surgical research 2013;181:e7.
26. Xu JZ, Qin H, Wang XQ, et al. Repair of large segmental bone defects using bone marrow stromal cells with demineralized bone matrix. Orthopaedic surgery 2009;1:34.
27. Quarto R, Mastrogiacomo M, Cancedda R, et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. The New England journal of medicine 2001;344:385.
28. Auer JA, Goodship A, Arnoczky S, et al. Refining animal models in fracture research: seeking consensus in optimising both animal welfare and scientific validity for appropriate biomedical use. BMC musculoskeletal disorders 2007;8:72.
29. Liu K, Li D, Huang X, et al. A murine femoral segmental defect model for bone tissue engineering using a novel rigid internal fixation system. The Journal of surgical research 2013;183:493.
30. Kinnaird T, Stabile E, Burnett MS, et al. Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circulation research 2004;94:678.
31. Wu Y, Wang J, Scott PG, Tredget EE. Bone marrow-derived stem cells in wound healing: a review. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2007;15(Suppl 1):S18.
32. Tasso R, Fais F, Reverberi D, Tortelli F, Cancedda R. The recruitment of two consecutive and different waves of host stem/progenitor cells during the development of tissueengineered bone in a murine model. Biomaterials 2010;31:2121.
33. Uusitalo H, Rantakokko J, Ahonen M, et al. A metaphyseal defect model of the femur for studies of murine bone healing. Bone 2001;28:423.
34. Laird DJ, von Andrian UH, Wagers AJ. Stem cell trafficking in tissue development, growth, and disease. Cell 2008;132:612.
35. Dupont KM, Sharma K, Stevens HY, Boerckel JD, Garcia AJ, Guldberg RE. Human stem cell delivery for treatment of large segmental bone defects. Proceedings of the National Academy of Sciences of the United States of America 2010;107:3305.