Rational design of a glycosynthase by the crystal structure of β-galactosidase from Bacillus circulans (BgaC) and its use for the synthesis of N-acetyllactosamine type 1 glycan structures

Journal of Biotechnology

Volumn 191, Issue , 2014, Pages 78-85

  Henze, Manja ^a   You, Dong Ju ^b,c   Kamerke, Claudia ^a   Hoffmann, Natalie ^d   Angkawidjaja, Clement ^c   Ernst, Sabrina ^a   Pietruszka, Jörg ^d,e   Kanaya, Shigenori ^c   Elling, Lothar ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b Korea Basic Science Institute (KBSI)   (South Korea)

^c OSAKA UNIVERSITY   (Japan)

^d HEINRICH HEINE UNIVERSITY   (Germany)

^e FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Bacillus circulans galactosidase; Crystal structure; Glycoconjugates; Glycosynthase

Indexed keywords

AMINO ACIDS; BACTERIOLOGY; ENZYME ACTIVITY; REGIOSELECTIVITY;

ENZYMATIC SYNTHESIS; GALACTOSIDASES; GLYCOCONJUGATES; GLYCOSIDE HYDROLASES; GLYCOSYNTHASE; REGIOSELECTIVE SYNTHESIS; SITE DIRECTED MUTAGENESIS; STREPTOCOCCUS PNEUMONIAE;

CRYSTAL STRUCTURE;

AMINO ACID; BETA GALACTOSIDASE; GALACTOSE; GLUTAMIC ACID; GLYCAN; GLYCOSIDASE; N ACETYLLACTOSAMINE SYNTHASE; AMINOSUGAR; N-ACETYLLACTOSAMINE; POLYSACCHARIDE;

ARTICLE; BACILLUS CIRCULANS; CRYSTAL STRUCTURE; ENZYME STRUCTURE; ENZYME SYNTHESIS; NONHUMAN; NUCLEOPHILICITY; SITE DIRECTED MUTAGENESIS; STREPTOCOCCUS PNEUMONIAE; STRUCTURE ANALYSIS; BACILLUS; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; PROTEIN FOLDING; X RAY CRYSTALLOGRAPHY;

BACILLUS CIRCULANS;

AMINO SUGARS; BACILLUS; BETA-GALACTOSIDASE; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; GALACTOSE; MUTAGENESIS, SITE-DIRECTED; POLYSACCHARIDES; PROTEIN FOLDING; SUBSTRATE SPECIFICITY;

EID: 84911903336     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.07.003     Document Type: Article

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