Label-free ITO-based immunosensor for the detection of very low concentrations of pathogenic bacteria

Bioelectrochemistry

Volumn 101, Issue , 2015, Pages 146-152

  Barreiros dos Santos, M ^a,b,c,d   Azevedo, S ^e   Agusil, J P ^a,d   Prieto Simon B ^a,d   Sporer, C ^a,d   Torrents, E ^a   Juarez A ^a,c   Teixeira, V ^e   Samitier, J ^a,c,d  

^a INSTITUTE FOR BIOENGINEERING OF CATALONIA IBEC   (Spain)

^b INTERNATIONAL IBERIAN NANOTECHNOLOGY LABORATORY   (Portugal)

^c UNIVERSITY OF BARCELONA   (Spain)

^d BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^e UNIVERSITY OF MINHO   (Portugal)

Author keywords

E. coli O157:H7; Electrochemical Impedance Spectroscopy; Immunosensor; Indium tin oxide; Label free detection

Indexed keywords

ANTIBODIES; ATOMIC FORCE MICROSCOPY; BACTERIA; CYCLIC VOLTAMMETRY; ELECTRON DEVICE MANUFACTURE; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; IMMUNOSENSORS; SPECTROSCOPY; TIN OXIDES;

E.COLI O157:H7; ESCHERICHIA COLI O157:H7; IMPEDIMETRIC IMMUNOSENSORS; INDIUM TIN OXIDE; LABEL-FREE DETECTION; MICRO CONTACT PRINTING; OPTICAL WAVEGUIDE LIGHT MODE SPECTROSCOPY; SALMONELLA TYPHIMURIUM;

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY;

BACTERIUM ANTIBODY; EPOXYSILANE; INDIUM TIN OXIDE; UNCLASSIFIED DRUG; IMMOBILIZED ANTIBODY;

ANTIGEN BINDING; ARTICLE; ATOMIC FORCE MICROSCOPY; BACTERIUM DETECTION; CYCLIC POTENTIOMETRY; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; ESCHERICHIA COLI O157; FLUORESCENCE MICROSCOPY; IMMUNOSENSOR; LIMIT OF DETECTION; OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY; REPRODUCIBILITY; SALMONELLA TYPHIMURIUM; SPECTROSCOPY; CHEMISTRY; DEVICES; ELECTRODE; EQUIPMENT DESIGN; ESCHERICHIA COLI K 12; IMMUNOASSAY; IMMUNOLOGY; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; PATHOGENICITY; PROCEDURES; SALMONELLA ENTERICA SEROVAR TYPHIMURIUM;

ANTIBODIES, BACTERIAL; ANTIBODIES, IMMOBILIZED; BACTERIOLOGICAL TECHNIQUES; DIELECTRIC SPECTROSCOPY; ELECTRODES; EQUIPMENT DESIGN; ESCHERICHIA COLI K12; ESCHERICHIA COLI O157; IMMUNOASSAY; LIMIT OF DETECTION; MICROSCOPY, ATOMIC FORCE; REPRODUCIBILITY OF RESULTS; SALMONELLA TYPHIMURIUM;

EID: 84911071704     PISSN: 15675394     EISSN: 1878562X     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2014.09.002     Document Type: Article

References (42)

1. Mannoor M.S., Zhang S., Link A.J., McAlpine M.C. Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides. Proc. Natl. Acad. Sci. U. S. A. 2010, 107:19207-19212.
2. Li D., Feng Y., Zhou L., Ye Z., Wang J., Ying Y., et al. Label-free capacitive immunosensor based on quartz crystal Au electrode for rapid and sensitive detection of Escherichia coli O157:H7. Anal. Chim. Acta. 2011, 687:89-96.
3. USDA Economic Research Service ERS/USDA Data Foodborne Illness Cost Calculator Available at:, (n.d.). http://webarchives.cdlib.org/sw1rf5mh0k/http://www.ers.usda.gov/Data/FoodborneIllness/.
4. Lazcka O., Del Campo F.J., Muñoz F.X. Pathogen detection: a perspective of traditional methods and biosensors. Biosens. Bioelectron. 2007, 22:1205-1217.
5. Mallotra B.D., Turner A.P.F. Advances in Biosensors Perspectives in Biosensors 2003, Elsevier Science B.V.
6. Kharitonov A.B., Alfonta L., Katz E., Willner I. Probing of bioaffinnity interactions at interfaces using impedance spectroscopy and chronopotentiometry. J. Electroanal. Chem. 2000, 487:133-141.
7. Katz E., Willner I. Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA-sensors, and enzyme biosensors. Electroanalysis 2003, 15:913-947.
8. Guan J.-G., Miao Y.-Q., Zhang Q.-J. Impedimetric biosensors. J. Biosci. Bioeng. 2004, 97:219-226.
9. Skottrup P.D., Nicolaisen M., Justesen A.F. Towards on-site pathogen detection using antibody-based sensors. Biosens. Bioelectron. 2008, 24:339-348.
10. Yang L., Bashir R. Electrical/electrochemical impedance for rapid detection of foodborne pathogenic bacteria. Biotechnol. Adv. 2008, 26:135-150.
11. Wang Y., Ye Z., Ying Y. New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria. Sensors (Basel) 2012, 12:3449-3471.
12. Sun X., Gillis K.D. On-chip amperometric measurement of quantal catecholamine release using transparent indium tin oxide electrodes. Anal. Chem. 2006, 78:2521-2525.
13. Adányi N., Varadi M., Kim N., Szendro I. Development of new immunosensors for determination of contaminants in food. Curr. Appl. Phys. 2006, 6:279-286.
14. Ruan C., Yang L., Li Y. Immunobiosensor chips for detection of Escherichia coli O157:H7 using Electrochemical Impedance Spectroscopy. Anal. Chem. 2002, 74:4814-4820.
15. Yang L., Li Y., Erf G.F. Interdigitated array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. Anal. Chem. 2004, 76:1107-1113.
16. Tokarskyy O., Marshall D.L. Immunosensors for rapid detection of Escherichia coli O157:H7 - perspectives for use in the meat processing industry. Food Microbiol. 2008, 25:1-12.
17. Ge B., Meng J. Advanced technologies for pathogen and toxin detection in foods: current applications and future directions. J. Assoc. Lab. Autom. 2009, 14:235-241.
18. 2 sputtered coatings on plastics for self-cleaning applications. Mater. Sci. Eng. B 2007, 138:144-150.
19. Horcas I., Fernández R., Gómez-Rodríguez J.M., Colchero J., Gómez-Herrero J., Baro A.M. WSXM: a software for scanning probe microscopy and a tool for nanotechnology. Rev. Sci. Instrum. 2007, 78:013705.
20. Caballero D., Pla-Roca M., Bessueille F., Mills C.A., Samitier J., Errachid A. Atomic force microscopy characterization of a microcontact printed, self-assembled thiol monolayer for use in biosensors. Anal. Lett. 2006, 39:1721-1734.
21. Hermanson G.T. Bioconjugate Techniques 2008, Academic Press. Second ed.
22. Tang H., Zhang W., Geng P., Wang Q., Jin L., Wu Z., et al. A new amperometric method for rapid detection of Escherichia coli density using a self-assembled monolayer-based bienzyme biosensor. Anal. Chim. Acta. 2006, 562:190-196.
23. Azevedo S., Diéguez L., Carvalho P., Carneiro J.O., Teixeira V., Martínez E., et al. Deposition of ITO thin films onto PMMA substrates for waveguide based biosensing devices. J. Nano Res. 2012, 17:75-83.
24. Daniels J.S., Pourmand N. Label-free impedance biosensors: opportunities and challenges. Electroanalysis 2007, 19:1239-1257.
25. Miller J., Miller J. Statistic and chemometrics for analytical chemistry 2005, Pearson Prentice Hall. Fifth ed.
26. Zhang D., Chen S., Qin L., Li R., Wang P., Li Y. The novel immunobiosensors for detection of Escherichia coli O157:H7 using Electrochemical Impedance Spectroscopy. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2005, 7:7111-7113.
27. Taylor A., Yu Q., Chen S., Homola J., Jiang S. Comparison of E. coli O157:H7 preparation methods used for detection with surface plasmon resonance sensor. Sensors Actuators B Chem. 2005, 107:202-208.
28. Waswa J., Irudayaraj J., Debroy C. Direct detection of E. Coli O157:H7 in selected food systems by a surface plasmon resonance biosensor. LWT Food Sci. Technol. 2007, 40:187-192.
29. Choi J., Oh B. Optical Detection of Pathogens using Protein Chip 2008, 348-362.
30. Su Y., Li X.L. A self-assembled monolayer-based piezoelectric immunosensor for rapid detection of Escherichia coli O157:H7. Biosens. Bioelectron. 2004, 19:563-574.
31. Shen Z.-Q., Wang J.-F., Qiu Z.-G., Jin M., Wang X.-W., Chen Z.-L., et al. QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold. Biosens. Bioelectron. 2011, 26:3376-3381.
32. Abdel-Hamid I., Ivnitski D., Atanasov P., Wilkins E. Flow-through immunofiltration assay system for rapid detection of E. coli O157:H7. Biosens. Bioelectron. 1999, 14(3):309-316.
33. Ruan C., Wang H., Li Y. A bienzyme electrochemical biosensor coupled with immunomagnatic separation for rapid detection of Escherichia coli O157:H7 in food samples. Trans. ASABE 2002, 45:249-255.
34. Gehring A.G., Patterson D.L., Tu S.I. Use of a light-addressable potentiometric sensor for the detection of Escherichia coli O157:H7. Anal. Biochem. 1998, 258:293-298.
35. Ercole C., Del Gallo M., Mosiello L., Baccella S., Lepidi A. Escherichia coli detection in vegetable food by a potentiometric biosensor. Sensors Actuators B Chem. 2003, 91:163-168.
36. Yang L., Li Y. AFM and impedance spectroscopy characterization of the immobilization of antibodies on indium-tin oxide electrode through self-assembled monolayer of epoxysilane and their capture of Escherichia coli O157:H7. Biosens. Bioelectron. 2005, 20:1407-1416.
37. Maalouf R., Fournier-Wirth C., Coste J., Chebib H., Saïkali Y., Vittori O., et al. Label-free detection of bacteria by electrochemical impedance spectroscopy: comparison to surface plasmon resonance. Anal. Chem. 2007, 79:4879-4886.
38. Geng P., Zhang X., Meng W., Wang Q., Zhang W., Jin L., et al. Self-assembled monolayers-based immunosensor for detection of Escherichia coli using electrochemical impedance spectroscopy. Electrochim. Acta 2008, 53:4663-4668.
39. Barreiros dos Santos M., Agusil J.P., Prieto-Simon B., Sporer C., Teixeira V., Samitier J. Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy. Biosens. Bioelectron. 2013, 45:174-180.
40. Moore E.J., Curtin M., Ionita J., Maguire A.R., Ceccone G., Galvin P. Selective release of DNA from the surface of indium-tin oxide thin electrode films using thiol-disulfide exchange chemistry. Anal. Chem. 2007, 79:2050-2057.
41. Nam Y., Branch D.W., Wheeler B.C. Epoxy-silane linking of biomolecules is simple and effective for patterning neuronal cultures. Biosens. Bioelectron. 2006, 22:589-597.
42. Solomun T., Mix R., Sturm H. Immobilization of silanized DNA on glass: influence of the silane tether on the DNA hybridization. ACS Appl. Mater. Interfaces 2010, 2:2171-2174.