Engineering of novel Staphylococcal Protein A ligands to enable milder elution pH and high dynamic binding capacity

Journal of Chromatography A

Volumn 1362, Issue , 2014, Pages 180-185

  Pabst, Timothy M ^a   Palmgren, Ronnie ^b   Forss, Annika ^b   Vasic, Jelena ^b   Fonseca, Mariko ^a   Thompson, Christopher ^a   Wang, William K ^a   Wang, Xiangyang ^a   Hunter, Alan K ^a  

^a MEDIMMUNE   (United States)

^b GE Healthcare   (Sweden)

Author keywords

Affinity chromatography; Antibody; Protein A; Z domain

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANTIBODIES; MOLECULES;

ACIDIC CONDITIONS; ALANINE MUTATIONS; DYNAMIC BINDING CAPACITY; FC FUSION PROTEINS; PROTEIN A; PROTEIN SEQUENCES; STAPHYLOCOCCAL PROTEIN A; Z-DOMAIN;

LIGANDS;

ALANINE; ASPARAGINE; HISTIDINE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; SERINE; STAPHYLOCOCCUS PROTEIN A; ANTIBODY; LIGAND; RECOMBINANT PROTEIN;

ACIDITY; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CULTURE MEDIUM; ELUTION; GEL PERMEATION CHROMATOGRAPHY; PH; POINT MUTATION; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN PURIFICATION; ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; PROCEDURES;

ANIMALS; ANTIBODIES; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETULUS; HYDROGEN-ION CONCENTRATION; LIGANDS; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A;

EID: 84908575348     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.08.046     Document Type: Article

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