Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of postprostatectomy erectile dysfunction

Journal of Sexual Medicine

Volumn 11, Issue 10, 2014, Pages 2439-2448

  Xu, Yongde ^a   Guan, Ruili ^a   Lei, Hongen ^a   Li, Huixi ^a   Wang, Lin ^a   Gao, Zhezhu ^a   Song, Weidong ^a   Xin, Zhongcheng ^a  

^a PEKING UNIVERSITY FIRST HOSPITAL   (China)

Author keywords

Adipose Derived Stem Cells; Cavernous Nerve Injury; Cellular Retention; Erectile Dysfunction; Hanging Drop; Micro Tissues; Paracrine Factors; Radical Prostatectomy

Indexed keywords

5 ETHYNYL 2 DEOXYURIDINE; CHEMOKINE RECEPTOR CXCR4; COLLAGEN; COLLAGEN TYPE 4; DEOXYURIDINE DERIVATIVE; NERVE GROWTH FACTOR; PHOSPHATE BUFFERED SALINE; PROTEIN; UNCLASSIFIED DRUG; VASCULOTROPIN C; WNT5A PROTEIN; VASCULOTROPIN A;

ADIPOSE DERIVED STEM CELL; ADIPOSE TISSUE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL AGGREGATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CORPUS CAVERNOSUM; CRUSH TRAUMA; ERECTILE DYSFUNCTION; FEASIBILITY STUDY; HISTOPATHOLOGY; IMMUNOHISTOLOGY; INTRACAVERNOUS PRESSURE; MALE; MALE GENITAL TRACT PARAMETERS; MICRO TISSUE; MICROSCOPY; NERVE INJURY; NERVE REGENERATION; NERVE STIMULATION; NONHUMAN; PENIS ERECTION; PENIS INJURY; PRESSURE AND TENSION; PRIORITY JOURNAL; PROSTATECTOMY; PROTEIN EXPRESSION; RAT; SHAM PROCEDURE; STEM CELL TRANSPLANTATION; TISSUE REPAIR; WESTERN BLOTTING; ADIPOCYTE; ADVERSE EFFECTS; ANIMAL; CYTOLOGY; METABOLISM; PATHOPHYSIOLOGY; PENIS; PHYSIOLOGY; PROCEDURES; SPRAGUE DAWLEY RAT; TRANSPLANTATION; VASCULARIZATION;

ADIPOCYTES; ADIPOSE TISSUE; ANIMALS; ERECTILE DYSFUNCTION; MALE; PENILE ERECTION; PENIS; PROSTATECTOMY; RATS; RATS, SPRAGUE-DAWLEY; STEM CELL TRANSPLANTATION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 84908503274     PISSN: 17436095     EISSN: 17436109     Source Type: Journal    
DOI: 10.1111/jsm.12636     Document Type: Article

References (27)

1. Hatzimouratidis K, Amar E, Eardley I, Giuliano F, Hatzichristou D, Montorsi F, Vardi Y, Wespes E. Guidelines on male sexual dysfunction: Erectile dysfunction and premature ejaculation. Eur Urol 2010;57:804-814.
2. Kendirci M, Bivalacqua TJ, Hellstrom WJ. Vardenafil: A novel type 5 phosphodiesterase inhibitor for the treatment of erectile dysfunction. Expert Opin Pharmacother 2004;5:923-932.
3. Jeong HH, Piao S, Ha JN, Kim IG, Oh SH, Lee JH, Cho HJ, Hong SH, Kim SW, Lee JY. Combined therapeutic effect of udenafil and adipose-derived stem cell (ADSC)/brain-derived neurotrophic factor (BDNF)-membrane system in a rat model of cavernous nerve injury. Urology 2013;81:e1107-1114.
4. Qiu X, Fandel TM, Ferretti L, Albersen M, Orabi H, Zhang H, Lin G, Lin CS, Schroeder T, Lue TF. Both immediate and delayed intracavernous injection of autologous adipose-derived stromal vascular fraction enhances recovery of erectile function in a rat model of cavernous nerve injury. Eur Urol 2012;62:720-727.
5. Montorsi F, Guazzoni G, Strambi LF, Da Pozzo LF, Nava L, Barbieri L, Rigatti P, Pizzini G, Miani A. Recovery of spontaneous erectile function after nerve-sparing radical retropubic prostatectomy with and without early intracavernous injections of alprostadil: Results of a prospective, randomized trial. J Urol 1997;158:1408-1410.
6. Muller A, Tal R, Donohue JF, Akin-Olugbade Y, Kobylarz K, Paduch D, Cutter SC, Mehrara BJ, Scardino PT, Mulhall JP. The effect of hyperbaric oxygen therapy on erectile function recovery in a rat cavernous nerve injury model. J Sex Med 2008;5:562-570.
7. Piao S, Kim IG, Lee JY, Hong SH, Kim SW, Hwang TK, Oh SH, Lee JH, Ra JC. Therapeutic effect of adipose-derived stem cells and BDNF-immobilized PLGA membrane in a rat model of cavernous nerve injury. J Sex Med 2012;9:1968-1979.
8. Kim SJ, Choi SW, Hur KJ, Park SH, Sung YC, Ha YS, Cho HJ, Hong SH, Lee JY, Hwang TK, Kim SW. Synergistic effect of mesenchymal stem cells infected with recombinant adenovirus expressing human BDNF on erectile function in a rat model of cavernous nerve injury. Korean J Urol 2012;53:726-732.
9. Kim IG, Piao S, Lee JY, Hong SH, Hwang TK, Kim SW, Kim CS, Ra JC, Noh I. Effect of an adipose-derived stem cell and nerve growth factor-incorporated hydrogel on recovery of erectile function in a rat model of cavernous nerve injury. Tissue Eng Part A 2013;19:14-23.
10. Potapova IA, Brink PR, Cohen IS, Doronin SV. Culturing of human mesenchymal stem cells as three-dimensional aggregates induces functional expression of CXCR4 that regulates adhesion to endothelial cells. J Biol Chem 2008;283:13100-13107.
11. Kelm JM, Diaz Sanchez-Bustamante C, Ehler E, Hoerstrup SP, Djonov V, Ittner L, Fussenegger M. VEGF profiling and angiogenesis in human microtissues. J Biotechnol 2005;118:213-229.
12. Frith JE, Thomson B, Genever PG. Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Eng Part C Methods 2010;16:735-749.
13. Bartosh TJ, Ylostalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, Lee RH, Choi H, Prockop DJ. Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci U S A 2010;107:13724-13729.
14. Huang YC, Shindel AW, Ning H, Lin G, Harraz AM, Wang G, Garcia M, Lue TF, Lin CS. Adipose derived stem cells ameliorate hyperlipidemia associated detrusor overactivity in a rat model. J Urol 2010;183:1232-1240.
15. Albersen M, Fandel TM, Lin G, Wang G, Banie L, Lin CS, Lue TF. Injections of adipose tissue-derived stem cells and stem cell lysate improve recovery of erectile function in a rat model of cavernous nerve injury. J Sex Med 2010;7:3331-3340.
16. Qiu X, Lin G, Xin Z, Ferretti L, Zhang H, Lue TF, Lin CS. Effects of low-energy shockwave therapy on the erectile function and tissue of a diabetic rat model. J Sex Med 2013;10:738-746.
17. Burnett AL. Rationale for cavernous nerve restorative therapy to preserve erectile function after radical prostatectomy. Urology 2003;61:491-497.
18. Leungwattanakij S, Bivalacqua TJ, Usta MF, Yang DY, Hyun JS, Champion HC, Abdel-Mageed AB, Hellstrom WJ. Cavernous neurotomy causes hypoxia and fibrosis in rat corpus cavernosum. J Androl 2003;24:239-245.
19. User HM, Hairston JH, Zelner DJ, McKenna KE, McVary KT. Penile weight and cell subtype specific changes in a post-radical prostatectomy model of erectile dysfunction. J Urol 2003;169:1175-1179.
20. Vignozzi L, Filippi S, Morelli A, Ambrosini S, Luconi M, Vannelli GB, Donati S, Crescioli C, Zhang XH, Mirone V, Forti G, Maggi M. Effect of chronic tadalafil administration on penile hypoxia induced by cavernous neurotomy in the rat. J Sex Med 2006;3:419-431.
21. Vignozzi L, Morelli A, Filippi S, Vannelli GB, Mungai S, Marini M, Boddi V, Forti G, Maggi M. Effect of sildenafil administration on penile hypoxia induced by cavernous neurotomy in the rat. Int J Impot Res 2008;20:60-67.
22. Kemp KC, Hows J, Donaldson C. Bone marrow-derived mesenchymal stem cells. Leuk Lymphoma 2005;46:1531-1544.
23. Lin G, Qiu X, Fandel T, Banie L, Wang G, Lue TF, Lin CS. Tracking intracavernously injected adipose-derived stem cells to bone marrow. Int J Impot Res 2011;23:268-275.
24. Fandel TM, Albersen M, Lin G, Qiu X, Ning H, Banie L, Lue TF, Lin CS. Recruitment of intracavernously injected adipose-derived stem cells to the major pelvic ganglion improves erectile function in a rat model of cavernous nerve injury. Eur Urol 2012;61:201-210.
25. Albersen M, Berkers J, Dekoninck P, Deprest J, Lue TF, Hedlund P, Lin CS, Bivalacqua TJ, Van Poppel H, De Ridder D. Expression of a distinct set of chemokine receptors in adipose tissue-derived stem cells is responsible for in vitro migration toward chemokines appearing in the major pelvic ganglion following cavernous nerve injury. Sex Med 2013;1:3-15.
26. Vignozzi L, Filippi S, Morelli A, Marini M, Chavalmane A, Fibbi B, Silvestrini E, Mancina R, Carini M, Vannelli GB, Forti G, Maggi M. Cavernous neurotomy in the rat is associated with the onset of an overt condition of hypogonadism. J Sex Med 2009;6:1270-1283.
27. Shindel AW, Xin ZC, Lin G, Fandel TM, Huang YC, Banie L, Breyer BN, Garcia MM, Lin CS, Lue TF. Erectogenic and neurotrophic effects of icariin, a purified extract of horny goat weed (Epimedium spp.) in vitro and in vivo. J Sex Med 2010;7:1518-1528.