Introducing transglycosylation activity in Bacillus licheniformis α-amylase by replacement of His235 with Glu

Biochemical and Biophysical Research Communications

Volumn 451, Issue 4, 2014, Pages 541-547

  Tran, Phuong Lan ^a   Cha, Hyun Ju ^b   Lee, Jin Sil ^a   Park, Sung Hoon ^c   Woo, Eui Jeon ^d   Park, Kwan Hwa ^a,e  

^a Sangmyung University   (South Korea)

^b Korea Food Research Institute   (South Korea)

^c Gachon University College of Medicine   (South Korea)

^d Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^e Seoul National University   (South Korea)

Author keywords

Bacillus licheniformis thermostable; Binding subsite mapping; Site directed mutagenesis; Substrate transglycosylation; Transfer product; amylase

Indexed keywords

AMYLASE; CARBON 14; ENZYME; ENZYME H235E; GLUCOSE; GLUTAMIC ACID; GLYCOSIDE; HISTIDINE; UNCLASSIFIED DRUG; ALPHA AMYLASE, BACILLUS LICHENIFORMIS; ALPHA-AMYLASE, BACILLUS LICHENIFORMIS; MALTOOCTAOSE; OLIGOSACCHARIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS LICHENIFORMIS; CATALYSIS; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; GLYCOSYLATION; HYDROLYSIS KINETICS; MOLECULAR MODEL; NONHUMAN; STATIC ELECTRICITY; WILD TYPE; AMINO ACID SEQUENCE; BACILLUS; BACILLUS LICHENIFORMIS THERMOSTABLE Α-AMYLASE; BINDING SITE; BINDING-SUBSITE MAPPING; ENZYMOLOGY; GENETICS; HYDROLYSIS; IDEOMOTOR APRAXIA; METABOLISM; SITE-DIRECTED MUTAGENESIS; SUBSTRATE TRANSGLYCOSYLATION; TRANSFER PRODUCT;

BACILLUS LICHENIFORMIS THERMOSTABLE Α-AMYLASE; BINDING-SUBSITE MAPPING; SITE-DIRECTED MUTAGENESIS; SUBSTRATE TRANSGLYCOSYLATION; TRANSFER PRODUCT;

ALPHA-AMYLASES; AMINO ACID SEQUENCE; APRAXIA, IDEOMOTOR; BACILLUS; BINDING SITES; GLUTAMIC ACID; GLYCOSYLATION; HISTIDINE; HYDROLYSIS; MODELS, MOLECULAR; OLIGOSACCHARIDES;

EID: 84908365836     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.08.019     Document Type: Article

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