A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

BMC Biotechnology

Volumn 14, Issue 1, 2014, Pages

  Kim, Juhan ^a   Webb, Anthony M ^a   Kershner, Jamie P ^a   Blaskowski, Stephen ^a   Copley, Shelley D ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Escherichia coli; Genome editing; I SceI; Salmonella enterica

Indexed keywords

ESCHERICHIA COLI; GENE ENCODING; SALMONELLA; TRANSCRIPTION;

DOUBLE STRAND BREAKS; HOMOLOGOUS RECOMBINATION; OPEN READING FRAME; OPTIMIZED PROTOCOL; POINT MUTATIONS; RECOMBINATION EVENTS; SALMONELLA ENTERICA; TRANSCRIPTION TERMINATORS;

GENES;

CODON; DNA TRANSCRIPTION; ESCHERICHIA COLI; ESSENTIAL GENE; GENE FREQUENCY; GENE MUTATION; GENETIC TRANSCRIPTION; GENOME; INDEL MUTATION; OPEN READING FRAME; PLASMID; POINT MUTATION; SALMONELLA ENTERICA; ARTICLE; BACTERIAL GENOME; GENE EXPRESSION REGULATION; GENETICS; METHODOLOGY; MUTATION;

ESCHERICHIA COLI; GENES, ESSENTIAL; GENOME, BACTERIAL; MUTAGENESIS, INSERTIONAL; MUTATION; PLASMIDS; SALMONELLA ENTERICA;

EID: 84908101863     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-84     Document Type: Article

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