A lentivirus-mediated miR-23b sponge diminishes the malignant phenotype of glioma cells in vitro and in vivo

Oncology Reports

Volumn 31, Issue 4, 2014, Pages 1573-1580

  Chen, Luyue ^b   Zhang, Kailiang ^b   Shi, Zhendong ^b   Zhang, Anling ^b   Jia, Zhifan ^b   Wang, Guangxiu ^b   Pu, Peiyu ^b   Kang, Chunsheng ^a   Han, Lei ^b  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

Glioblastoma; Malignant phenotype; MicroRNA sponge; MicroRNA 23b

Indexed keywords

BETA CATENIN; GELATINASE A; GELATINASE B; HYPOXIA INDUCIBLE FACTOR 1ALPHA; LENTIVIRUS VECTOR; MICRORNA; MICRORNA 23B SPONGE; TRANSCRIPTION FACTOR ZEB1; UNCLASSIFIED DRUG; UVOMORULIN; VASCULOTROPIN; VON HIPPEL LINDAU PROTEIN;

ANGIOGENESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL FUNCTION; CELL INVASION; CELL MIGRATION; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENE THERAPY; GLIOMA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MALIGNANT NEOPLASTIC DISEASE; MOUSE; NONHUMAN; PHENOTYPE; PLEIOTROPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA ANALYSIS; RNA BINDING; TUMOR GROWTH;

ANIMALS; BLOTTING, WESTERN; BRAIN NEOPLASMS; CELL LINE, TUMOR; GENE KNOCKDOWN TECHNIQUES; GLIOMA; HETEROGRAFTS; HUMANS; IMMUNOHISTOCHEMISTRY; LENTIVIRUS; MICE; MICE, NUDE; MICRORNAS; PHENOTYPE; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84907934338     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2014.3012     Document Type: Article

References (27)

1. Krützfeldt J, Rajewsky N, Braich R, et al: Silencing of microRNAs in vivo with 'antagomirs'. Nature 438: 685-689, 2005.
2. Meister G, Landthaler M, Dorsett Y and Tuschl T: Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA 3: 544-550, 2004.
3. Ørom UA, Kauppinen S and Lund AH: LNA-modified oligonucleotides mediate specific inhibition of microRNA function. Gene 372: 137-141, 2006.
4. Ebert MS, Neilson JR and Sharp PA: MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods 9: 721-726, 2007.
5. Ebert MS and Sharp PA: MicroRNA sponges: progress and possibilities. RNA 16: 2043-2050, 2010.
6. Valastyan S, Reinhardt F, Benaich N, et al: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Cell 137: 1032-1046, 2009.
7. Lin J, Teo S, Lam DH, Jeyaseelan K and Wang S: MicroRNA-10b pleiotropically regulates invasion, angiogenicity and apoptosis of tumor cells resembling mesenchymal subtype of glioblastoma multiforme. Cell Death Dis 3: e398, 2012.
8. Zhang H, Hao Y, Yang J, et al: Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis. Nat Commun 2: 554, 2011.
9. Xu W, Liu M, Peng X, et al: miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells via cooperative regulation of MXI1. Int J Oncol 42: 757-766, 2013.
10. Furnari FB, Fenton T, Bachoo RM, et al: Malignant astrocytic glioma: genetics, biology, and paths to treatment. Genes Dev 21: 2683-2710, 2007.
11. Chen L, Han L, Zhang K, et al: VHL regulates the effects of miR-23b on glioma survival and invasion via suppression of HIF-1/VEGF and β-catenin/Tcf-4 signaling. Neuro Oncol 14: 1026-1036, 2012.
12. Ciafrè SA, Galardi S, Mangiola A, et al: Extensive modulation of a set of microRNAs in primary glioblastoma. Biochem Biophys Res Commun 334: 1351-1358, 2005.
13. Wang KC, Garmire LX, Young A, et al: Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth. Proc Natl Acad Sci USA 107: 3234-3239, 2010.
14. Jin L, Wessely O, Marcusson EG, Ivan C, Calin GA and Alahari SK: Prooncogenic factors miR-23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-α in breast cancer. Cancer Res 73: 2884-2896, 2013.
15. Rogler CE, LeVoci L, Ader T, et al: MicroRNA-23b cluster microRNAs regulate transforming growth factor-beta/bone morphogenetic protein signaling and liver stem cell differentiation by targeting Smads. Hepatology 50: 575-584, 2009.
16. Zhou X, Ren Y, Moore L, et al: Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest 90: 144-155, 2010.
17. Rubinson DA, Dillon CP, Kwiatkowski AV, et al: A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 33: 401-406, 2003.
18. Dull T, Zufferey R, Kelly M, et al: A third-generation lentivirus vector with a conditional packaging system. J Virol 72: 8463-8471, 1998.
19. Hutson TE, Davis ID, Machiels JH, et al: Biomarker analysis and final efficacy and safety results of a phase II renal cell carcinoma trial with pazopanib (GW786034), a multi-kinase angiogenesis inhibitor. J Clin Oncol 158: 5046, 2008.
20. Lu Z, Ghosh S, Wang Z and Hunter T: Downregulation of caveolin-1 function by EGF leads to the loss of E-cadherin, increased transcriptional activity of β-catenin, and enhanced tumor cell invasion. Cancer Cell 4: 499-515, 2003.
21. Burk U, Schubert J, Wellner U, et al: A reciprocal repression between ZEB1 and members of the miR-200 family promotes EMT and invasion in cancer cells. EMBO Rep 9: 582-589, 2008.
22. Wang L, Zhang ZG, Zhang RL, et al: Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J Neurosci 26: 5996-6003, 2006.
23. Kota J, Chivukula RR, O'Donnell KA, et al: Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell 137: 1005-1017, 2009.
24. Zhang J, Han L, Ge Y, et al: miR-221/222 promote malignant progression of glioma through activation of the Akt pathway. Int J Oncol 36: 913-920, 2010.
25. Geng J, Luo H, Pu Y, et al: Methylation mediated silencing of miR-23b expression and its role in glioma stem cells. Neurosci Lett 528: 185-189, 2012.
26. Foxler DE, Bridge KS, James V, et al: The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity. Nat Cell Biol 14: 201-208, 2012.
27. Schmalhofer O, Brabletz S and Brabletz T: E-cadherin, β-catenin, and ZEB1 in malignant progression of cancer. Cancer Metastasis Rev 28: 151-166, 2009.