Dynamic expression of desmin, α-SMA and TGF-β1 during hepatic fibrogenesis induced by selective bile duct ligation in young rats

Brazilian Journal of Medical and Biological Research

Volumn 47, Issue 10, 2014, Pages 850-857

  Goncalves J O ^a   Tannuri, A C A ^a   Coelho, M C M ^a   Bendit, I ^a   Tannuri, U ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Alpha smooth muscle actin; Desmin; Hepatic fibrogenesis; RT PCR; Selective bile duct ligation; TGF 1

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COMPLEMENTARY DNA; CYCLOPHILIN; DESMIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; TRANSFORMING GROWTH FACTOR BETA1; ACTIN; COLLAGEN TYPE 1; SMOOTH MUSCLE ACTIN, RAT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BILE DUCT LIGATION; CHOLESTASIS; CONTROLLED STUDY; FEMALE; FIBROGENESIS; GENE AMPLIFICATION; GENE EXPRESSION; HEPATIC FIBROGENESIS; MALE; NONHUMAN; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; ANALYSIS OF VARIANCE; ANIMAL; BILE DUCT; BILE DUCT ATRESIA; BIOSYNTHESIS; COMPLICATION; DISEASE MODEL; GENETICS; LIGATION; LIVER; LIVER CIRRHOSIS; METABOLISM; PROCEDURES; SURGERY; WISTAR RAT;

ACTINS; ANALYSIS OF VARIANCE; ANIMALS; BILE DUCTS; BILIARY ATRESIA; CHOLESTASIS; COLLAGEN TYPE I; DESMIN; DISEASE MODELS, ANIMAL; FEMALE; GENE EXPRESSION; LIGATION; LIVER; LIVER CIRRHOSIS; MALE; RATS, WISTAR; REAL-TIME POLYMERASE CHAIN REACTION; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84907572383     PISSN: None     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/1414-431X20143679     Document Type: Article

References (31)

1. Tannuri AC, Coelho MC, de Oliveira Goncalves J, Santos MM, Ferraz da Silva LF, Bendit I, et al. Effects of selective bile duct ligation on liver parenchyma in young animals: histologic and molecular evaluations. J Pediatr Surg 2012; 47: 513-522, doi: 10.1016/j.jpedsurg.2011.10.009.
2. Tannuri U, Velhote MC, Santos MM, Gibelli NE, Ayoub AA, Maksoud-Filho JG, et al. Pediatric liver transplantation: fourteen years of experience at the children institute in Sao Paulo, Brazil. Transplant Proc 2004; 36: 941-942, doi: 10.1016/j.transproceed.2004.03.101.
3. Karakayali F, Kirnap M, Akdur A, Tutar N, Boyvat F, Moray G, et al. Biliary complications after pediatric liver transplantation. Transplant Proc 2013; 45: 3524-3527, doi: 10.1016/j.transproceed.2013.09.012.
4. Wadhawan M, Kumar A, Gupta S, Goyal N, Shandil R, Taneja S, et al. Post-transplant biliary complications: an analysis from a predominantly living donor liver transplant center. J Gastroenterol Hepatol 2013; 28: 1056-1060, doi: 10.1111/jgh.12169.
5. Glaser S, DeMorrow S, Francis H, Ueno Y, Gaudio E, Vaculin S, et al. Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms. Am J Physiol Gastrointest Liver Physiol 2008; 295: G124-G136, doi: 10.1152/ajpgi.00536.2007.
6. Marzioni M, Glaser S, Francis H, Marucci L, Benedetti A, Alvaro D, et al. Autocrine/paracrine regulation of the growth of the biliary tree by the neuroendocrine hormone serotonin. Gastroenterology 2005; 128: 121-137, doi: 10.1053/j.gastro.2004.10.002.
7. Gaudio E, Barbaro B, Alvaro D, Glaser S, Francis H, Ueno Y, et al. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism. Gastroenterology 2006; 130: 1270-1282, doi: 10.1053/j.gastro.2005.12.034.
8. Novo E, di Bonzo LV, Cannito S, Colombatto S, Parola M. Hepatic myofibroblasts: a heterogeneous population of multifunctional cells in liver fibrogenesis. Int J Biochem Cell Biol 2009; 41: 2089-2093, doi: 10.1016/j.biocel.2009.03.010.
9. Ballardini G, Fallani M, Biagini G, Bianchi FB, Pisi E. Desmin and actin in the identification of Ito cells and in monitoring their evolution to myofibroblasts in experimental liver fibrosis. Virchows Arch B Cell Pathol Incl Mol Pathol 1988; 56: 45-49, doi: 10.1007/BF02890000.
10. Henderson NC, Sheppard D. Integrin-mediated regulation of TGFbeta in fibrosis. Biochim Biophys Acta 2013; 1832: 891- 896, doi: 10.1016/j.bbadis.2012.10.005.
11. Rosenbloom J, Mendoza FA, Jimenez SA. Strategies for anti-fibrotic therapies. Biochim Biophys Acta 2013; 1832: 1088-1103, doi: 10.1016/j.bbadis.2012.12.007.
12. COBEA (Colégio Brasileiro de Experimentação Animal). Ethic Commission for animal experimentation. http://www.cobea.org.br.
13. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408, doi: 10.1006/meth.2001.1262.
14. Chobert MN, Couchie D, Fourcot A, Zafrani ES, Laperche Y, Mavier P, et al. Liver precursor cells increase hepatic fibrosis induced by chronic carbon tetrachloride intoxication in rats. Lab Invest 2012; 92: 135-150, doi: 10.1038/labinvest.2011.143.
15. Golbar HM, Izawa T, Yano R, Ichikawa C, Sawamoto O, Kuwamura M, et al. Immunohistochemical characterization of macrophages and myofibroblasts in alpha-Naphthylisothiocyanate (ANIT) - induced bile duct injury and subsequent fibrogenesis in rats. Toxicol Pathol 2011; 39: 795-808, doi: 10.1177/0192623311413790.
16. Gibelli NE, Tannuri U, Mello ES. Immunohistochemical studies of stellate cells in experimental cholestasis in newborn and adult rats. Clinics 2008; 63: 689-694, doi: 10.1590/S1807-59322008000500019.
17. Iredale JP, Thompson A, Henderson NC. Extracellular matrix degradation in liver fibrosis: Biochemistry and regulation. Biochim Biophys Acta 2013; 1832: 876-883, doi: 10.1016/j.bbadis.2012.11.002.
18. Ballardini G, Groff P, Badiali de Giorgi L, Schuppan D, Bianchi FB. Ito cell heterogeneity: desmin-negative Ito cells in normal rat liver. Hepatology 1994; 19: 440-446, doi: 10.1002/hep.1840190224.
19. Takase S, Leo MA, Nouchi T, Lieber CS. Desmin distinguishes cultured fat-storing cells from myofibroblasts, smooth muscle cells and fibroblasts in the rat. J Hepatol 1988; 6: 267-276, doi: 10.1016/S0168-8278(88)80042-4.
20. Gressner AM. Transdifferentiation of hepatic stellate cells (Ito cells) to myofibroblasts: a key event in hepatic fibrogenesis. Kidney Int Suppl 1996; 54: S39-S45.
21. Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol 2011; 25: 195-206, doi: 10.1016/j.bpg.2011.02.005.
22. Kaimori A, Potter J, Kaimori JY, Wang C, Mezey E, Koteish A. Transforming growth factor-beta1 induces an epithelialto- mesenchymal transition state in mouse hepatocytes in vitro. J Biol Chem 2007; 282: 22089-22101, doi: 10.1074/jbc.M700998200.
23. Lewindon PJ, Pereira TN, Hoskins AC, Bridle KR, Williamson RM, Shepherd RW, et al. The role of hepatic stellate cells and transforming growth factor-beta(1) in cystic fibrosis liver disease. Am J Pathol 2002; 160: 1705-1715, doi: 10.1016/S0002-9440(10)61117-0.
24. Lijnen P, Petrov V. Transforming growth factor-beta 1- induced collagen production in cultures of cardiac fibroblasts is the result of the appearance of myofibroblasts. Methods Find Exp Clin Pharmacol 2002; 24: 333-344, doi: 10.1358/mf.2002.24.6.693065.
25. Meindl-Beinker NM, Dooley S. Transforming growth factorbeta and hepatocyte transdifferentiation in liver fibrogenesis. J Gastroenterol Hepatol 2008; 23 (Suppl 1): S122-S127, doi: 10.1111/j.1440-1746.2007.05297.x.
26. Dooley S, Delvoux B, Lahme B, Mangasser-Stephan K, Gressner AM. Modulation of transforming growth factor beta response and signaling during transdifferentiation of rat hepatic stellate cells to myofibroblasts. Hepatology 2000; 31: 1094-1106, doi: 10.1053/he.2000.6126.
27. Jakowlew SB, Mead JE, Danielpour D, Wu J, Roberts AB, Fausto N. Transforming growth factor-beta (TGF-beta) isoforms in rat liver regeneration: messenger RNA expression and activation of latent TGF-beta. Cell Regul 1991; 2: 535-548.
28. Schrum LW, Bird MA, Salcher O, Burchardt ER, Grisham JW, Brenner DA, et al. Autocrine expression of activated transforming growth factor-beta(1) induces apoptosis in normal rat liver. Am J Physiol Gastrointest Liver Physiol 2001; 280: G139-G148.
29. Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal Biochem 2000; 285: 194-204, doi: 10.1006/abio.2000.4753.
30. Dagher H, Donninger H, Hutchinson P, Ghildyal R, Bardin P. Rhinovirus detection: comparison of real-time and conventional PCR. J Virol Methods 2004; 117: 113-121, doi: 10.1016/j.jviromet.2004.01.003.
31. Bachem MG, Meyer D, Melchior R, Sell KM, Gressner AM. Activation of rat liver perisinusoidal lipocytes by transforming growth factors derived from myofibroblastlike cells. A potential mechanism of self perpetuation in liver fibrogenesis. J Clin Invest 1992; 89: 19-27, doi: 10.1172/JCI115561.