Rhinovirus detection: Comparison of real-time and conventional PCR

Journal of Virological Methods

Volumn 117, Issue 2, 2004, Pages 113-121

  Dagher, Hayat ^a   Donninger, Howard ^b,c   Hutchinson, Paul ^a   Ghildyal, Reena ^a   Bardin, Philip ^a  

^a MONASH MEDICAL CENTRE   (Australia)

^b STELLENBOSCH UNIVERSITY   (South Africa)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Real time PCR; Rhinovirus; SYBR Green I

Indexed keywords

VIRUS DNA;

ACCURACY; ARTICLE; CHILD; CONTROLLED STUDY; DILUTION; DNA DETERMINATION; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHINOVIRUS; VIRUS DETECTION; VIRUS ISOLATION;

COMMON COLD; FLOW CYTOMETRY; HELA CELLS; HUMANS; NASAL MUCOSA; NUCLEIC ACID DENATURATION; PICORNAVIRIDAE INFECTIONS; POLYMERASE CHAIN REACTION; RHINOVIRUS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

RHINOVIRUS;

EID: 1642342855     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.01.003     Document Type: Article

References (25)

1. Atmar R.L., Guy E., Guntupalli K.K., Zimmerman J.L., Bandi V.D., Baxter B.D., Greenberg S.B. Respiratory tract viral infections in inner-city asthmatic adults. Arch. Intern. Med. 158(22):1998;2453-2459.
2. Bates P.J., Sanderson G., Holgate S.T., Johnston S.L. A comparison of RT-PCR, in-situ hybridisation and in-situ RT-PCR for the detection of rhinovirus infection in paraffin sections. J. Virol. Methods. 67(2):1997;153-160.
3. Blomqvist S., Skytta A., Roivainen M., Hovi T. Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription-PCR-hybridization assay. J. Clin. Microbiol. 37(9):1999;2813-2816.
4. Couch, B.R., 2001. Rhinoviruses. In: Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., Straus, S.E. (Eds), Fields Virology, 4th Edition, Vol. 1, Lippincott Williams and Wilkins, pp. 777-797.
5. Donninger H., Glashoff R., Haitchi H.-M., Syce J.A., Ghildyal R., Rensburg E.V., Bardin P.G. Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium. J. Infect. Dis. 187(11):2003;1809-1817.
6. Freymuth F., Vabret A., Brouard J., Toutain F., Verdon R., Petitjean J., Gouarin S., Duhamel J.F., Guillois B. Detection of viral, Chlamydia pneumoniae and Mycoplasma pneumoniae infections in exacerbations of asthma in children. J. Clin. Virol. 13(3):1999;131-139.
7. Freymuth F., Vabret A., Galateau-Salle F., Ferey J., Eugene G., Petitjean J., Gennetay E., Brouard J., Jokik M., Duhamel J.F., Guillois B. Detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization. Clin. Diagn. Virol. 8(1):1997;31-40.
8. Gilbert L.L., Dakhama A., Bone B.M., Thomas E.E., Hegele R.G. Diagnosis of viral respiratory tract infections in children by using a reverse transcription-PCR panel. J. Clin. Microbiol. 34(1):1996;140-143.
9. Hyypia T., Puhakka T., Ruuskanen O., Makela M., Arola A., Arstila P. Molecular diagnosis of human rhinovirus infections: comparison with virus isolation. J. Clin. Microbiol. 36(7):1998;2081-2083.
10. Ireland D.C., Kent J., Nicholson K.G. Improved detection of rhinoviruses in nasal and throat swabs by seminested RT-PCR. J. Med. Virol. 40(2):1993;96-101.
11. Johnston S.L., Pattemore P.K., Sanderson G., Smith S., Lampe F., Josephs L., Symington P., O'Toole S., Myint S.H., Tyrrell D.A., Holgate S.T. Community study of role of viral infections in exacerbations of asthma in 9-11 year old children. Br. Med. J. 310(6989):1995;1225-1229.
12. Johnston S.L., Sanderson G., Pattemore P.K., Smith S., Bardin P.G., Bruce C.B., Lambden P.R., Tyrrell D.A., Holgate S.T. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J. Clin. Microbiol. 31(1):1993;111-117.
13. Komurian-Pradel F., Paranhos-Baccala G., Sodoyer M., Chevallier P., Mandrand B., Lotteau V., Andre P. Quantitation of HCV RNA using real-time PCR and fluorimetry. J. Virol. Methods. 95(1-2):2001;111-119.
14. Lee W.M., Wang W., Rueckert R.R. Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes. 9(2):1995;177-181.
15. Lonnrot M., Sjoroos M., Salminen K., Maaronen M., Hyypia T., Hyoty H. Diagnosis of enterovirus and rhinovirus infections by RT-PCR and time-resolved fluorometry with lanthanide chelate labeled probes. J. Med. Virol. 59(3):1999;378-384.
16. McMillan J.A., Weiner L.B., Higgins A.M., Macknight K. Rhinovirus infection associated with serious illness among pediatric patients. Pediatr. Infect. Dis. J. 12(4):1993;321-325.
17. Nicholson K.G., Kent J., Hammersley V., Cancio E. Acute viral infections of upper respiratory tract in elderly people living in the community: comparative, prospective, population based study of disease burden. Br. Med. J. 25:1997;1060-1064.
18. Nicholson K.G., Kent J., Ireland D.C. Respiratory viruses and exacerbations of asthma in adults. Br. Med. J. 307(6910):1993;982-986.
19. Nijhuis M., van Maarseveen N., Schuurman R., Verkuijlen S., de Vos M., Hendriksen K., van Loon A.M. Rapid and sensitive routine detection of all members of the genus enterovirus in different clinical specimens by real-time PCR. J. Clin. Microbiol. 40(10):2002;3666-3670.
20. Papadopoulos N.G., Hunter J., Sanderson G., Meyer J., Johnston S.L. Rhinovirus identification by BglI digestion of picornavirus RT-PCR amplicons. J. Virol. Methods. 80(2):1999;179-185.
21. Pitkaranta A., Arruda E., Malmberg H., Hayden F.G. Detection of rhinovirus in sinus brushings of patients with acute community-acquired sinusitis by reverse transcription-PCR. J. Clin. Microbiol. 35(7):1997;1791- 1793.
22. Pitkaranta A., Virolainen A., Jero J., Arruda E., Hayden F.G. Detection of rhinovirus, respiratory syncytial virus and coronavirus infections in acute otitis media by reverse transcriptase polymerase chain reaction. Pediatrics. 102(2 Pt 1):1998;291-295.
23. Racaniello, V.R., 2001. Picornaviridae: The viruses and their replication. In: Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., Straus, S.E. (Eds), Fields Virology, 4th Edition, Vol. 1, Lippincott Williams and Wilkins, pp. 685-722.
24. Rohr U.-P., Wulf M.-A., Stahn S., Steidl U., Haas R., Kronenwett R. Fast and reliable titration of recombinant adeno-associated virus type-II using quantitative real-time PCR. J. Virol. Methods. 106(1):2002;81-88.
25. Verstrepen W.A., Kuhn S., Kockx M.M., Van De Vyvere M.E., Mertens A.H. Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay. J. Clin. Microbiol. 39(11):2001;4093-4096.