Escherichia coli as a glycoprotein production host: Recent developments and challenges

Current Opinion in Biotechnology

Volumn 30, Issue , 2014, Pages 205-210

  Jaffé, Stephen R P ^a   Strutton, Benjamin ^a   Levarski, Zdenko ^b   Pandhal, Jagroop ^a   Wright, Phillip C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b Physics and Informatics   (Slovakia)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; GLYCAN; GLYCOPROTEIN; OLIGOSACCHARYLTRANSFERASE; RECOMBINANT PROTEIN; TRANSFERASE; UNCLASSIFIED DRUG;

BACTERIAL CELL; CAMPYLOBACTER JEJUNI; CARBOHYDRATE ANALYSIS; CARBOHYDRATE SYNTHESIS; CHO CELL LINE; ESCHERICHIA COLI; HOST CELL; NONHUMAN; PROKARYOTE; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; REVIEW; ANIMAL; CRICETULUS; GENETICS; GLYCOSYLATION; HUMAN; METABOLISM; MICROBIOLOGY;

ANIMALS; CAMPYLOBACTER JEJUNI; CHO CELLS; CRICETULUS; ESCHERICHIA COLI; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; INDUSTRIAL MICROBIOLOGY; RECOMBINANT PROTEINS;

EID: 84907270784     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2014.07.006     Document Type: Review

References (49)

1. Aggarwal S. What's fueling the biotech engine-2012 to 2013. Nat Biotechnol 2014, 32:32-39.
2. Pandhal J., Wright P.C. N-Linked glycoengineering for human therapeutic proteins in bacteria. Biotechnol Lett 2010, 32:1189-1198.
3. Solá R.J., Griebenow K. Glycosylation of therapeutic proteins: an effective strategy to optimize efficacy. BioDrugs 2011, 24:9-21.
4. Stork R., Zettlitz K.A., Müller D., Rether M., Hanisch F.-G., Kontermann R.E. N-glycosylation as novel strategy to improve pharmacokinetic properties of bispecific single-chain diabodies. J Biol Chem 2008, 283:7804-7812.
5. Forthal D.N., Gach J.S., Landucci G., Jez J., Strasser R., Kunert R., Steinkellner H. Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12. J Immunol 2010, 185:6876-6882.
6. Varki A. Glycan-based interactions involving vertebrate sialic-acid-recognizing proteins. Nature 2007, 446:1023-1029.
7. Kim J.Y., Kim Y-G., Lee G.M. CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl Microbiol Biotechnol 2012, 93:917-930.
8. Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M., Wang W., Hammond S., et al. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat Biotechnol 2011, 29:735-741.
9. Noh S.M., Sathyamurthy M., Lee G.M. Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production. Curr Opin Chem Eng 2013, 2:391-397.
10. Wacker M., Linton D., Hitchen P.G., Nita-Lazar M., Haslam S.M., North S.J., Panico M., Morris H.R., Dell A., Wren B.W., et al. N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science 2002, 298:1790-1793.
11. Faridmoayer A., Fentabil M.a., Haurat M.F., Yi W., Woodward R., Wang P.G., Feldman M.F. Extreme substrate promiscuity of the Neisseria oligosaccharyl transferase involved in protein O-glycosylation. J Biol Chem 2008, 283:34596-34604.
12. Cuccui J., Thomas R. Exploitation of bacterial N-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against Francisella tularensis. Open Biol 2013, 3.
13. Kowarik M., Young N.M., Numao S., Schulz B.L., Hug I., Callewaert N., Mills D.C., Watson D.C., Hernandez M., Kelly J.F., et al. Definition of the bacterial N-glycosylation site consensus sequence. EMBO J 2006, 25:1957-1966.
14. Feldman M.F., Wacker M., Hernandez M., Hitchen P.G., Marolda C.L., Kowarik M., Morris H.R., Dell A., Valvano M.a., Aebi M. Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli. Proc Natl Acad Sci U S A 2005, 102:3016-3021.
15. Nothaft H., Szymanski C.M. Protein glycosylation in bacteria: sweeter than ever. Nat Rev Microbiol 2010, 8:765-778.
16. Nothaft H., Szymanski C.M. Bacterial protein N-glycosylation: new perspectives and applications. J Biol Chem 2013, 288:6912-6920.
17. Schwarz F., Huang W., Li C., Schulz B.L., Lizak C., Palumbo A., Numao S., Neri D., Aebi M., Wang L-X. A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation. Nat Chem Biol 2010, 6:264-266.
18. Pandhal J., Woodruff L.B.A., Jaffe S., Desai P., Ow S.Y., Noirel J., Gill R.T., Wright P.C. Inverse metabolic engineering to improve Escherichia coli as an N-glycosylation host. Biotechnol Bioeng 2013, 110:2482-2493.
19. Robinson C., Matos C.F.R.O., Beck D., Ren C., Lawrence J., Vasisht N., Mendel S. Transport and proofreading of proteins by the twin-arginine translocation (Tat) system in bacteria. Biochim Biophys Acta 2011, 1808:876-884.
20. Fisher A.C., Kim J.-Y., Perez-Rodriguez R., Tullman-Ercek D., Fish W.R., Henderson L.a., DeLisa M.P. Exploration of twin-arginine translocation for expression and purification of correctly folded proteins in Escherichia coli. Microb Biotechnol 2008, 1:403-415.
21. Zhang G., Brokx S., Weiner J.H. Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nat Biotechnol 2006, 24:100-104.
22. Fisher A.C., Haitjema C.H., Guarino C., Çelik E., Endicott C.E., Reading C.A., Merritt J.H., Ptak A.C., Zhang S., DeLisa M.P. Production of secretory and extracellular N-linked glycoproteins in Escherichia coli. Appl Environ Microbiol 2011, 77:871-881.
23. Nita-Lazar M., Wacker M., Schegg B., Amber S., Aebi M. The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology 2005, 15:361-367.
24. Schwarz F., Lizak C., Fan Y-Y., Fleurkens S., Kowarik M., Aebi M. Relaxed acceptor site specificity of bacterial oligosaccharyltransferase in vivo. Glycobiology 2011, 21:45-54.
25. Ielmini M.V., Feldman M.F. Desulfovibrio desulfuricans PglB homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements. Glycobiology 2011, 21:734-742.
26. Han R., Li J., Shin H.-D., Chen R.R., Du G., Liu L., Chen J. Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications. Biotechnol Adv 2013, 10.1016/j.biotechadv.2013.12.004.
27. Gerber S., Lizak C., Michaud G., Bucher M., Darbre T., Aebi M., Reymond J-L., Locher K.P. Mechanism of bacterial oligosaccharyltransferase: in vitro quantification of sequon binding and catalysis. J Biol Chem 2013, 288:8849-8861.
28. Lizak C., Gerber S., Numao S., Aebi M., Locher K.P. X-ray structure of a bacterial oligosaccharyltransferase. Nature 2011, 474:350-355.
29. Ihssen J., Kowarik M., Wiesli L., Reiss R., Wacker M., Thöny-Meyer L. Structural insights from random mutagenesis of Campylobacter jejuni oligosaccharyltransferase PglB. BMC Biotechnol 2012, 12:67.
30. Hansen E.H., Osmani S.a., Kristensen C., Møller B.L., Hansen J. Substrate specificities of family 1 UGTs gained by domain swapping. Phytochemistry 2009, 70:473-482.
31. Latemple D.C., Abrams J.T., Zhang S.Y. Increased immunogenicity of tumor vaccines complexed with anti-gal: studies in knockout mice for αlpha1,3Galactosyltransferase. Cancer Res 1999, 59:3417-3423.
32. Comstock L.E., Kasper D.L. Bacterial glycans: key mediators of diverse host immune responses. Cell 2006, 126:847-850.
33. Valderrama-Rincon J., Fisher A., Merritt J., Fan Y-Y., Reading C., Chhiba K., Heiss C., Azadi P., Aebi M., DeLisa M.P. An engineered eukaryotic protein glycosylation pathway in Escherichia coli. Nat Chem Biol 2012, 8:434-436.
34. Linton D., Dorrell N., Hitchen P.G., Amber S., Karlyshev A.V., Morris H.R., Dell A., Valvano M.A., Aebi M., Wren B.W. Functional analysis of the Campylobacter jejuni N-linked protein glycosylation pathway. Mol Microbiol 2005, 55:1695-1703.
35. Li B., Zeng Y., Hauser S., Song H., Wang L-X. Highly efficient endoglycosidase-catalyzed synthesis of glycopeptides using oligosaccharide oxazolines as donor substrates. J Am Chem Soc 2005, 127:9692-9693.
36. Couto J., Huffaker T., Robbins P. Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway. J Biol Chem 1984, 259:378-382.
37. O'Reilly M., Zhang G., Imperiali B. In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis. Biochemistry 2006, 14:9593-9603.
38. Wang X., Weldeghiorghis T., Zhang G. Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase. Structure 2008, 16:965-975.
39. Tyo K.E.J., Ajikumar P.K., Stephanopoulos G. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nat Biotechnol 2009, 27:760-765.
40. Pandhal J., Desai P., Walpole C., Doroudi L., Malyshev D., Wright P.C. Systematic metabolic engineering for improvement of glycosylation efficiency in Escherichia coli. Biochem Biophys Res Commun 2012, 419:472-476.
41. Feldman M.F., Wacker M., Hernandez M., Hitchen P.G., Marolda C.L., Kowarik M., Morris H.R., Dell A., Valvano M.A., Aebi M. Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli. Proc Natl Acad Sci U S A 2005, 102:3016-3021.
42. Ihssen J., Kowarik M., Dilettoso S., Tanner C., Wacker M., Thöny-Meyer L. Production of glycoprotein vaccines in Escherichia coli. Microb Cell Fact 2010, 9.
43. Gstaiger M., Aebersold R. Applying mass spectrometry-based proteomics to genetics, genomics and network biology. Nat Rev Genet 2009, 10:617-627.
44. Aebersold R., Burlingame A.L., Bradshaw R.A. Western blots versus selected reaction monitoring assays: time to turn the tables?. Mol Cell Proteomics 2013, 12:2381-2382.
45. Sanda M., Pompach P., Brnakova Z. Quantitative LC-MS-MRM analysis of site-specific glycoforms of haptoglobin in liver disease. Mol Cell Proteomics 2013, 12:1294-1305.
46. Song E., Pyreddy S., Mechref Y. Quantification of glycopeptides by multiple reaction monitoring LC-MS/MS. Rapid Commun Mass Spectrom 2013, 26:1941-1954.
47. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Biotechnology 1979, 76:4350-4354.
48. Ahn Y.H., Shin P.M., Ji E.S., Kim H., Yoo J.S. A lectin-coupled, multiple reaction monitoring based quantitative analysis of human plasma glycoproteins by mass spectrometry. Anal Bioanal Chem 2012, 402:2101-2112.
49. Pan S., Aebersold R., Chen R. Mass spectrometry based targeted protein quantification: methods and applications. J Proteome Res 2009, 8:787-797.