Circulating immature granulocytes with T-cell killing functions predict sepsis deterioration

Critical Care Medicine

Volumn 42, Issue 9, 2014, Pages 2007-2018

  Guérin, Estelle ^a   Orabona, Marie ^a   Raquil, Marie Astrid ^b   Giraudeau, Bruno ^c   Bellier, Rémy ^a   Gibot, Sébastien ^d   Béné, Marie Christine ^d   Lacombe, Francis ^e   Droin, Nathalie ^b   Solary, Eric ^b   Vignon, Philippe ^a   Feuillard, Jean ^a   François, Bruno ^a  

^a DUPUYTREN UNIVERSITY HOSPITAL   (France)

^b Universite Paris Sud   (France)

^c UNIVERSITY HOSPITAL OF TOURS   (France)

^d UNIVERSITY HOSPITAL OF NANCY   (France)

^e HAUT LÉVÊQUE HOSPITAL   (France)

Author keywords

biomarker; flow cytometry; granulocytes; leucocytes; prognosis; sepsis

Indexed keywords

CD16 ANTIGEN; CD24 ANTIGEN; CD64 ANTIGEN; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN;

ADULT; ARTICLE; CELL KILLING; CELL POPULATION; CONTROLLED STUDY; DETERIORATION; EMERGENCY WARD; FEMALE; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; HUMAN CELL; IMMATURE GRANULOCYTE; IMMUNOSUPPRESSIVE TREATMENT; INTENSIVE CARE UNIT; LYMPHOCYTOPENIA; MAJOR CLINICAL STUDY; MALE; MORTALITY; MYELOID DERIVED SUPPRESSOR CELL; PRIORITY JOURNAL; PROGNOSIS; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY; SEPSIS; SUPPRESSOR CELL; SURVIVAL RATE; T LYMPHOCYTE;

ADULT; AGED; AGED, 80 AND OVER; BIOLOGICAL MARKERS; FEMALE; GRANULOCYTES; HUMANS; LEUKOCYTE COUNT; MALE; MIDDLE AGED; PROGNOSIS; SEPSIS; SURVIVAL ANALYSIS; T-LYMPHOCYTES;

EID: 84906791003     PISSN: 00903493     EISSN: 15300293     Source Type: Journal    
DOI: 10.1097/CCM.0000000000000344     Document Type: Article

References (67)

1. Martin GS, Mannino DM, Eaton S, et al: The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003; 348:1546-1554
2. Angus DC, Wax RS: Epidemiology of sepsis: An update. Crit Care Med 2001; 29:S109-S116
3. Dombrovskiy VY, Martin AA, Sunderram J, et al: Rapid increase in hospitalization and mortality rates for severe sepsis in the United States: A trend analysis from 1993 to 2003. Crit Care Med 2007; 35:1244-1250
4. Dellinger RP, Levy MM, Carlet JM, et al: Surviving Sepsis Campaign: International guidelines for management of severe sepsis and septic shock: 2008. Intensive Care Med 2008; 34:17-60
5. Annane D, Bellissant E, Cavaillon JM: Septic shock. Lancet 2005; 365:63-78
6. Hotchkiss RS, Opal S: Immunotherapy for sepsis-A new approach against an ancient foe. N Engl J Med 2010; 363:87-89
7. Delano MJ, Scumpia PO, Weinstein JS, et al: MyD88-dependent expansion of an immature GR-1(+)CD11b(+) population induces T cell suppression and Th2 polarization in sepsis. J Exp Med 2007; 204:1463-1474
8. Orr Y, Wilson DP, Taylor JM, et al: A kinetic model of bone marrow neutrophil production that characterizes late phenotypic maturation. Am J Physiol Regul Integr Comp Physiol 2007; 292:R1707-R1716
9. Bone RC, Balk RA, Cerra FB, et al: Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 1992; 101:1644-1655
10. Cornbleet PJ: Clinical utility of the band count. Clin Lab Med 2002; 22:101-136
11. Seebach JD, Morant R, Rüegg R, et al: The diagnostic value of the neutrophil left shift in predicting inflammatory and infectious disease. Am J Clin Pathol 1997; 107:582-591
12. Roussel M, Gros A, Gacouin A, et al: Toward new insights on the white blood cell differential by flow cytometry: A proof of concept study on the sepsis model. Cytometry B Clin Cytom 2012; 82:345-352
13. Hirsh M, Mahamid E, Bashenko Y, et al: Overexpression of the high-affinity Fcgamma receptor (CD64) is associated with leukocyte dysfunction in sepsis. Shock 2001; 16:102-108
14. Davis BH, Olsen SH, Ahmad E, et al: Neutrophil CD64 is an improved indicator of infection or sepsis in emergency department patients. Arch Pathol Lab Med 2006; 130:654-661
15. Danikas DD, Karakantza M, Theodorou GL, et al: Prognostic value of phagocytic activity of neutrophils and monocytes in sepsis. Correlation to CD64 and CD14 antigen expression. Clin Exp Immunol 2008; 154:87-97
16. Icardi M, Erickson Y, Kilborn S, et al: CD64 index provides simple and predictive testing for detection and monitoring of sepsis and bacterial infection in hospital patients. J Clin Microbiol 2009; 47:3914-3919
17. Döcke WD, Höflich C, Davis KA, et al: Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression: A multicenter standardized study. Clin Chem 2005; 51:2341-2347
18. Faucher JL, Lacronique-Gazaille C, Frébet E, et al: "6 markers/5 colors" extended white blood cell differential by flow cytometry. Cytometry A 2007; 71:934-944
19. Fingerle G, Pforte A, Passlick B, et al: The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis patients. Blood 1993; 82:3170-3176
20. Skinner NA, MacIsaac CM, Hamilton JA, et al: Regulation of Toll-like receptor (TLR)2 and TLR4 on CD14dimCD16+ monocytes in response to sepsis-related antigens. Clin Exp Immunol 2005; 141:270-278
21. Ziegler-Heitbrock L: The CD14+ CD16+ blood monocytes: Their role in infection and inflammation. J Leukoc Biol 2007; 81:584-592
22. Venet F, Lepape A, Monneret G: Clinical review: Flow cytometry perspectives in the ICU-From diagnosis of infection to monitoring of injury-induced immune dysfunctions. Crit Care 2011; 15:231
23. Schwulst SJ, Muenzer JT, Chang KC, et al: Lymphocyte phenotyping to distinguish septic from nonseptic critical illness. J Am Coll Surg 2008; 206:335-342
24. Unsinger J, Kazama H, McDonough JS, et al: Differential lymphopenia-induced homeostatic proliferation for CD4+ and CD8+ T cells following septic injury. J Leukoc Biol 2009; 85:382-390
25. Guisset O, Dilhuydy MS, Thiébaut R, et al: Decrease in circulating dendritic cells predicts fatal outcome in septic shock. Intensive Care Med 2007; 33:148-152
26. Jaye DL, Bray RA, Gebel HM, et al: Translational applications of flow cytometry in clinical practice. J Immunol 2012; 188:4715-4719
27. Levy MM, Fink MP, Marshall JC, et al; SCCM/ESICM/ACCP/ATS/SIS: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med 2003; 31:1250-1256
28. Ponder E, Mineola L: The polycyte. J Lab Clin Med 1942; 27:866
29. Orfanakis NG, Ostlund RE, Bishop CR, et al: Normal blood leukocyte concentration values. Am J Clin Pathol 1970; 53:647-651
30. Droin N, Jacquel A, Hendra JB, et al: Alpha-defensins secreted by dysplastic granulocytes inhibit the differentiation of monocytes in chronic myelomonocytic leukemia. Blood 2010; 115:78-88
31. Valleron A: Introduction à la biostatistique. Paris, Masson, 1988
32. Valleron A: Probabilité et Statistique. Paris, Masson, 2001
33. Kaplan EL, Meier P: Nonparametric estimation from incomplete observation. J Am Stat Assoc 1958; 53:457-481
34. Cox DR: Regression models and life-tables. J Stat Assoc 1972; 34:187-220
35. Lyn-Kew K, Standiford TJ: Immunosuppression in sepsis. Curr Pharm Des 2008; 14:1870-1881
36. Reddy RC, Chen GH, Tekchandani PK, et al: Sepsis-induced immunosuppression: From bad to worse. Immunol Res 2001; 24:273-287
37. Gibot S, Béné MC, Noel R, et al: Combination biomarkers to diagnose sepsis in the critically ill patient. Am J Respir Crit Care Med 2012; 186:65-71
38. Della Bella S, Giannelli S, Taddeo A, et al: Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays. J Immunol Methods 2008; 339:153-164
39. Oberholzer A, Steckholzer U, Kurimoto M, et al: Interleukin-18 plasma levels are increased in patients with sepsis compared to severely injured patients. Shock 2001; 16:411-414
40. Silveira RC, Procianoy RS: Evaluation of interleukin-6, tumour necrosis factor-alpha and interleukin-1beta for early diagnosis of neonatal sepsis. Acta Paediatr 1999; 88:647-650
41. Marchant A, Alegre ML, Hakim A, et al: Clinical and biological significance of interleukin-10 plasma levels in patients with septic shock. J Clin Immunol 1995; 15:266-273
42. Gogos CA, Drosou E, Bassaris HP, et al: Pro-versus anti-inflammatory cytokine profile in patients with severe sepsis: A marker for prognosis and future therapeutic options. J Infect Dis 2000; 181:176-180
43. Orr Y, Taylor JM, Bannon PG, et al: Circulating CD10-/CD16 low neutrophils provide a quantitative index of active bone marrow neutrophil release. Br J Haematol 2005; 131:508-519
44. Terstappen LW, Safford M, Loken MR: Flow cytometric analysis of human bone marrow. III. Neutrophil maturation. Leukemia 1990; 4:657-663
45. Arnoulet C, Béné MC, Durrieu F, et al: Four-and five-color flow cytometry analysis of leukocyte differentiation pathways in normal bone marrow: A reference document based on a systematic approach by the GTLLF and GEIL. Cytometry B Clin Cytom 2010; 78:4-10
46. Drifte G, Dunn-Siegrist I, Tissières P, et al: Innate immune functions of immature neutrophils in patients with sepsis and severe systemic inflammatory response syndrome. Crit Care Med 2013; 41: 820-832
47. Linderkamp O, Ruef P, Brenner B, et al: Passive deformability of mature, immature, and active neutrophils in healthy and septicemic neonates. Pediatr Res 1998; 44:946-950
48. Rodwell RL, Taylor KM, Tudehope DI, et al: Hematologic scoring system in early diagnosis of sepsis in neutropenic newborns. Pediatr Infect Dis J 1993; 12:372-376
49. Cavallazzi R, Bennin CL, Hirani A, et al: Is the band count useful in the diagnosis of infection? An accuracy study in critically ill patients. J Intensive Care Med 2010; 25:353-357
50. Park DH, Park K, Park J, et al: Screening of sepsis using leukocyte cell population data from the Coulter automatic blood cell analyzer DxH800. Int J Lab Hematol 2011; 33:391-399
51. Elghetany MT: Surface antigen changes during normal neutrophilic development: A critical review. Blood Cells Mol Dis 2002; 28:260-274
52. Gratama JW, Orfao A, Barnett D, et al: Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry 1998; 34:128-142
53. Frébet E, Abraham J, Geneviève F, et al; GEIL Groupe d'Etude Immunologique des Leucémies Study Group: A GEIL flow cytometry consensus proposal for quantification of plasma cells: Application to differential diagnosis between MGUS and myeloma. Cytometry B Clin Cytom 2011; 80:176-185
54. Ansari-Lari MA, Kickler TS, Borowitz MJ: Immature granulocyte measurement using the Sysmex XE-2100. Relationship to infection and sepsis. Am J Clin Pathol 2003; 120:795-799
55. Nierhaus A, Klatte S, Linssen J, et al: Revisiting the white blood cell count: Immature granulocytes count as a diagnostic marker to discriminate between SIRS and sepsis-A prospective, observational study. BMC Immunol 2013; 14:8
56. Heffernan DS, Monaghan SF, Thakkar RK, et al: Failure to normalize lymphopenia following trauma is associated with increased mortality, independent of the leukocytosis pattern. Crit Care 2012; 16:R12
57. Giamarellos-Bourboulis EJ: What is the pathophysiology of the septic host upon admission? Int J Antimicrob Agents 2010; 36(Suppl 2): S2-S5
58. Hotchkiss RS, Osmon SB, Chang KC, et al: Accelerated lymphocyte death in sepsis occurs by both the death receptor and mitochondrial pathways. J Immunol 2005; 174:5110-5118
59. Hotchkiss RS, Tinsley KW, Swanson PE, et al: Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol 2001; 166:6952-6963
60. Le Tulzo Y, Pangault C, Gacouin A, et al: Early circulating lymphocyte apoptosis in human septic shock is associated with poor outcome. Shock 2002; 18:487-494
61. Hotchkiss RS, Swanson PE, Freeman BD, et al: Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction. Crit Care Med 1999; 27:1230-1251
62. Wyllie DH, Bowler IC, Peto TE: Relation between lymphopenia and bacteraemia in UK adults with medical emergencies. J Clin Pathol 2004; 57:950-955
63. Ayala A, Lomas JL, Grutkoski PS, et al: Fas-ligand mediated apoptosis in severe sepsis and shock. Scand J Infect Dis 2003; 35: 593-600
64. Ward NS, Casserly B, Ayala A: The compensatory anti-inflammatory response syndrome (CARS) in critically ill patients. Clin Chest Med 2008; 29:617-625, viii
65. Herzig DS, Guo Y, Fang G, et al: Therapeutic efficacy of CXCR3 blockade in an experimental model of severe sepsis. Crit Care 2012; 16:R168
66. Cuenca AG, Delano MJ, Kelly-Scumpia KM, et al: A paradoxical role for myeloid-derived suppressor cells in sepsis and trauma. Mol Med 2011; 17:281-292
67. Gachard N, Salviat A, Boutet C, et al; GEIL: Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method. Haematologica 2008; 93:215-223