Rapid analysis of chromosome aberrations in mouse B lymphocytes by PNA-FISH

Journal of Visualized Experiments

Volumn , Issue 90, 2014, Pages

  Misenko, Sarah M ^a   Bunting, Samuel F ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

DNA repair; FISH; Genomic instability; Immunology; Issue 90; Metaphase spread; Mouse; Primary culture

Indexed keywords

EID: 84906347099     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51806     Document Type: Article

References (16)

1. Sancar, A., Lindsey-Boltz, L. A., Unsal-Kacmaz, K., & Linn, S. Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Ann. Rev. Biochem. 73, 39-85, doi:10.1146/annurev.biochem.73.011303.073723, (2004).
2. Sperka, T., Wang, J., & Rudolph, K. L. DNA damage checkpoints in stem cells, ageing and cancer. Nature reviews. Mol. Cell Biol. 13(9), 579-590, doi:10.1038/nrm3420, (2012).
3. Alexandrov, L. B. et al. Signatures of mutational processes in human cancer. Nature. 500(7463), 415-421, doi:10.1038/nature12477, (2013).
4. Bunting, S. F. et al. 53BP1 inhibits homologous recombination in Brca1-deficient cells by blocking resection of DNA breaks. Cell. 141(2), 243-254, doi:10.1016/j.cell.2010.03.012, (2010).
5. Padilla-Nash, H. M., Barenboim-Stapleton, L., Difilippantonio, M. J., & Ried, T. Spectral karyotyping analysis of human and mouse chromosomes. Nat. Protoc. 1(6), 3129-3142, doi:10.1038/nprot.2006.358, (2006).
6. Callen, E. et al. Chimeric IgH-TCRalpha/delta translocations in T lymphocytes mediated by RAG. Cell Cycle. 8(15), 2408-2412 (2009).
7. Boboila, C. et al. Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70. Proc. Natl. Acad. Sci. U.S.A. 107(7), 3034-3039, doi:10.1073/pnas.0915067107, (2010).
8. Benn, P., & Delach, J. Human lymphocyte culture and chromosome analysis. Cold Spring Harb Protoc. 2008, doi:10.1101/pdb.prot5035, (2008).
9. Nguyen, H. N., & Reijo Pera, R. A. Metaphase spreads and spectral karyotyping of human embryonic stem cells. Cold Spring Harb Protoc. 2008, doi:10.1101/pdb.prot5047, (2008).
10. Schreck, R. R., & Disteche, C. M. Chromosome banding techniques. Curr. Protoc. Hum Genet. Chapter 4, Unit4 2, doi:10.1002/0471142905.hg0402s00 (2001).
11. Gilley, D. et al. DNA-PKcs is critical for telomere capping. Proc. Natl. Acad. Sci. U.S.A. 98(26), 15084-15088, doi:10.1073/pnas.261574698, (2001).
12. Bolzan, A. D. Chromosomal aberrations involving telomeres and interstitial telomeric sequences. Mutagenesis. 27(1), 1-15, doi:10.1093/mutage/ger052, (2012).
13. Bolzan, A. D., & Bianchi, M. S. Telomeres, interstitial telomeric repeat sequences, and chromosomal aberrations. Mutat Res. 612(3), 189-214, doi:10.1016/j.mrrev.2005.12.003, (2006).
14. Poon, S. S., & Lansdorp, P. M. Quantitative fluorescence in situ hybridization (Q-FISH). Curr. Protoc. Cell Biol. Chapter 18, Unit 18 14, doi:10.1002/0471143030.cb1804s12, (2001).
15. Morales, J. C. et al. 53BP1 and p53 synergize to suppress genomic instability and lymphomagenesis. Proc. Natl. Acad. Sci. U.S.A. 103(9), 3310-3315, doi:10.1073/pnas.0511259103, (2006).
16. Halaby, M. J. et al. Synergistic interaction of Rnf8 and p53 in the protection against genomic instability and tumorigenesis. PLoS Genet. 9(1), e1003259, doi:10.1371/journal.pgen.1003259, (2013).