Characterization of secondary structure and fad conformational state in free and sol-gel immobilized glucose oxidase

Journal of Sol-Gel Science and Technology

Volumn 71, Issue 3, 2014, Pages 580-588

  Portaccio, Marianna ^a   Esposito, Rosario ^b   Delfino, Ines ^c   Lepore, Maria ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c UNIVERSITY OF TUSCIA   (Italy)

Author keywords

FT IR spectroscopy; Glucose oxidase; Secondary structure; Tertiary structure; Time resolved fluorescence

Indexed keywords

GLUCOSE OXIDASE; GLUCOSE SENSORS; INFRARED SPECTROSCOPY; SOL-GEL PROCESS; SOL-GELS;

FLAVIN ADENINE DINUCLEOTIDE; FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY; FTIR SPECTROSCOPY; OPTICAL SPECTROSCOPIC TECHNIQUES; SECONDARY STRUCTURES; STRUCTURAL AND DYNAMIC PROPERTIES; TERTIARY STRUCTURES; TIME-RESOLVED FLUORESCENCE;

ENZYME IMMOBILIZATION;

EID: 84906059153     PISSN: 09280707     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10971-014-3408-3     Document Type: Article

References (48)

1. Brinker J, Scherer GW (1990) The physics and chemistry of sol gel processing. Academic Press, New York
2. Dave BC, Dunn B, Valentine JS, Zink JI (1998) Sol-gel matrices for protein entrapment. In: Cass T, Ligier FS (eds) Immobilized biomolecules in analysis, a practical approach. Oxford University, Oxford
3. Pierre AC (2004) The sol-gel encapsulation of enzymes. Biocatal Biotransform 22:145-170
4. Mac Craith BD, McDonagh C, McEvoy AK, Butler T, O'Keeffe G, Murphy V (1997) Optical chemical sensors based on sol-gel materials: recent advances and critical issues. J Solgel Sci Technol 8:1053-1061
5. Jin W, Brennan JD (2002) Properties and applications of proteins encapsulated within sol-gel derived materials. Anal Chim Acta 461:1-36
6. Sakka S (2005) Handbook of Sol-gel science and technology: processing, characterization and applications. Kluwer Academic Publisher, New York (USA)
7. Prodromis MI, Karayannis MI (2002) Enzyme based amperometric biosensors for food analysis. Electroanalysis 14:241-261
8. Chaudhury NK, Bhardwaj R, Murari BM (2003) Fluorescence spectroscopic study to characterize and monitor TEOS based sol-gel process for development of optical biosensors. Curr Appl Phys 3:177-184
9. Tatsu Y, Yamashita K, Yamaguchi M, Yamamura S, Yamamoto H, Yoshikawa S (1992) Entrapment of glucose oxidase in silica gel by the sol-gel method and its application to glucose sensor. Chem Lett 21:1615-1618
10. Narang U, Prasad PN, Bright FV, Ramanathan K, Kumar DK, Malhotra BD, Kamalahasan MN, Chandra S (1994) Glucose biosensor based on a sol-gel-derived platform. Anal Chem 66:3139-3144
11. Portaccio M, Lepore M, Della Ventura B, Stoilova O, Manolova N, Rashkov I, Mita DG (2009) Fiber-optic glucose biosensor based on glucose oxidase immobilised in a silica gel matrix. J Solgel Sci Technol 50:437-448
12. Esposito R, Della Ventura B, De Nicola S, Altucci C, Velotta R, Mita DG, Lepore M (2011) Glucose sensing based on the intrinsic time-resolved visible fluorescence of sol-gel immobilized glucose oxidase. Sensors 11:3483-3497
13. Lim J, Malati P, Bonet F, Dunn B (2007) Nanostructured sol-gel electrodes for biofuel cells. J Electrochem Soc 154:A140-A145
14. 2 biofuel cell based on graphene and multiwalled carbon nanotube composite modified electrode. Int J Electrochem Sci 7:8064-8075
15. Hartnett AM, Ingersoll CM, Baker GA, Bright FV (1999) Kinetics and thermodynamics of free flavins and the flavins-based redox active site within glucose oxidase dissolved in solution or sequestered within a sol-gel derived glass. Anal Chem 71:1215-1224
16. Przybyt M (2003) Behaviour of glucose oxidase during formation and ageing of silica gel studied by fluorescence spectroscopy. Mater Sci 21:397-416
17. Han K, Wu Z, Lee J, Ahn I-S, Park JW, Min BR, Lee K (2005) Activity of glucose oxidase entrapped in mesoporous gels. Biochem Eng J 22:161-166
18. Przybyt M, Miller E, Szreder T (2011) Thermostability of glucose oxidase in silica gel obtained by sol-gel method and in solution studied by fluorimetric method. J Photochem Photobiol B Biol 103:22-28
19. Portaccio M, Della Ventura B, Mita DG, Manolova N, Stoilova O, Rashkov I, Lepore M (2011) FT-IR microscopy characterization of sol-gel layers prior and after glucose oxidase immobilization for biosensing applications. J Solgel Sci Technol 57:204-211
20. Delfino I, Portaccio M, Della Ventura B, Mita DG, Lepore M (2013) Enzyme distribution and secondary structure of sol-gel immobilized glucose oxidase by means of micro-attenuated total reflection FT-IR spectroscopy. Mater Sci Eng C 33:304-310. doi: 10.1016/j.msec.2012.08.044
21. Zuo S, Teng Y, Yuan H, Lan M (2008) Direct electrochemistry of glucose oxidase on screen-printed electrodes through one-step enzyme immobilization process with silica sol-gel/polyvinyl alcohol hybrid film. Sens Actuators B 133:555-560
22. Kong T, Chen Y, Ye Y, Zhang K, Wang Z, Wang X (2009) An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes. Sens Actuators B 138:344-350
23. Dirix C, Duvetter T, van Loey A, Hendrickx M, Heremans K (2005) The in situ observation of the temperature and pressure stability of recombinant Aspergillus aculeatus pectin methylesterase with Fourier transform IR spectroscopy reveals an unusual pressure stability of b-helices. Biochem J 392:565-571. doi: 10.1042/BJ20050721
24. Zhao H-Z, Sun J-J, Song J, Yang Q-Z (2010) Direct electron transfer and conformational change of glucose oxidase on carbon nanotube-based electrodes. Carbon 48:1508-1514
25. Smith AW (2012) Lipid-protein interactions in biological membranes: a dynamic perspective. Biochim Biophys Acta 118:172-177
26. Engelborghs Y (2001) The analysis of time resolved protein fluorescence in multi-tryptophan proteins. Spectrochim Acta A Mol Biomol Spectrosc 57:2255-2270
27. Esposito R, Delfino I, Lepore M (2013) Time-resolved flavin adenine dinucleotide fluorescence study of the interaction between immobilized glucose oxidase and glucose. J Fluoresc 23:947-955. doi: 10.1007/s10895-013-1220-z
28. Shao Q, Wu P, Xu X, Zhang H, Cai C (2012) Insight into the effects of graphene oxide sheets on the conformation and activity of glucose oxidase: towards developing a nanomaterial-based protein conformation assay. Phys Chem Chem Phys 14:9076-9085
29. Perkin Elmer Inc. (2004) Applications and Design of a Micro-ATR objective: Technical Note http://www.perkinelmer.com/CMSResources/Images/44- 4861TCH-MicroATRObjective.pdf
30. Levenberg K (1944) A method for the estimation of certain non-linear problems in least squares. Q Appl Math 2:164-168
31. Marquardt DW (1963) An algorithm for least squares estimation of nonlinear parameters. ESIAM J Appl Math 11:431-441
32. Zhong D, Zewail AH (2001) Femtosecond dynamics of flavoproteins: charge separation and recombination in riboflavine (Vitamin B2)-binding protein and in glucose oxidase enzyme. Proc Natl Acad Sci USA 98:11867-11872
33. Lepore M, Portaccio M, De Tommasi E, De Luca P, Bencivenga U, Maiuri P, Mita DG (2004) Glucose concentration determination by means of fluorescence emission spectra of soluble and insoluble glucose oxidase: some useful indications for optical fibre-based sensors. J Mol Catal B Enzym 31:151-158
34. Trettnak W, Wolfbeis OS (1989) Fully reversible fiber-optic glucose biosensor based on the intrinsic fluorescence of glucose oxidase. Anal Chim Acta 211:195-203
35. Started G (1997) MATLAB The Language of Technical Computing. Components, L(7):186
36. Qingwen L, Guoan L, Yiming W, Xingrong Z (2000) Immobilization of glucose oxidase in sol-gel matrix and its application to fabricate chemiluminescent glucose sensor. Mater Sci Eng C 11:67-70. doi: 10.1016/S0928-4931(00)00130-2
37. Barth A (2007) Infrared spectroscopy of proteins. Biochim Biophys Acta Bioenerg 1767:1073-1101
38. Krimm S, Bandekar J (1986) Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Adv Protein Chem 38:181-364
39. Susi H, Byler DM (1986) Resolution-enhanced Fourier transform infrared spectroscopy of enzymes. Methods Enzymol 130:290-311
40. Mei Y, Miller L, Gao W, Gross RA (2003) Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy. Biomacromolecules 4:70-74. doi: 10.1021/bm025611t
41. Jurgen-Lohmann DL, Nacke C, Legge RL, Simon LC (2009) Preparation and methodolodgy for chemical mapping of sol-gel thin films containing lysozyme. J Solgel Sci Technol 50:77-86
42. Kong T, Chen Y, Ye Y, Zhang K, Wang Z, Wang X (2009) An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes. Sens Actuators B 138:344-350
43. Liang R-P, Jiang J-L, Qiu J-D (2008) Preparation of GOD/Sol-Gel silica film on prussian blue modified electrode for glucose biosensor application. Electroanalysis 20:2642-2648. doi: 10.1002/elan.200804370
44. Li J, Wang YB, Qiu J-D, Sun DC, Xia XH (2005) Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor. Anal Bioanal Chem 383:918-922
45. Jackson M, Mantsch HH (1995) The use and misuse of FTIR spectroscopy in the determination of protein structure. Crit Rev Biochem Mol Biol 30:95-120. doi: 10.3109/10409239509085140
46. Hecht HJ, Kalisz HM, Hendle J, Schmid RD, Schomburg D (1993) Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 A resolution. J Mol Biol 229:153-172
47. van de Weert M, Haris PI, Hennink WE, Crommelin DJ (2001) Fourier transform infrared spectrometric analysis of protein conformation: effect of sampling method and stress factors. Anal Biochem 297:160-169
48. Gouda MD, Singh SA, Rao AGA, Thakurt MS, Karanth NG (2003) Thermal inactivation of glucose oxidase: mechanism and stabilization using additives. J Biol Chem 278:24324-24333. doi: 10.1074/jbc.M208711200