Effects of pyruvate dehydrogenase subunits overexpression on the α-ketoglutarate production in Yarrowia lipolytica WSH-Z06

Applied Microbiology and Biotechnology

Volumn 98, Issue 16, 2014, Pages 7003-7012

  Guo, Hongwei ^a,b   Madzak, Catherine ^c   Du, Guocheng ^a,b   Zhou, Jingwen ^a,b   Chen, Jian ^a,b  

^a JIANGNAN UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

^c CNRS   (France)

Author keywords

Acetyl CoA; Pyruvate; Pyruvate dehydrogenase complex; Unbalanced assembly; Yarrowia lipolytica; Ketoglutarate

Indexed keywords

BIOTECHNOLOGY;

ACETYL-COA; KETOGLUTARATE; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVATES; YARROWIA LIPOLYTICA;

BIOLOGY;

2 OXOGLUTARIC ACID; ACETYL COENZYME A; CARBON; COCARBOXYLASE; OXOGLUTARATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE E1; PYRUVATE DEHYDROGENASE E2; PYRUVATE DEHYDROGENASE E3; PYRUVIC ACID; THIAMINE; UNCLASSIFIED DRUG;

COMPETITION (ECOLOGY); CONCENTRATION (COMPOSITION); ENZYME ACTIVITY; GENE EXPRESSION;

ARTICLE; BACTERIUM CULTURE; CELL GROWTH; CITRIC ACID CYCLE; ENZYME SUBUNIT; ENZYME SYNTHESIS; FUNGAL STRAIN; FUNGUS GROWTH; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; YARROWIA LIPOLYTICA;

YARROWIA LIPOLYTICA;

EID: 84905980651     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5745-0     Document Type: Article

References (31)

1. Balakrishnan A, Nemeria NS, Chakraborty S, Kakalis L, Jordan F (2012) Determination of pre-steady-state rate constants on the Escherichia coli pyruvate dehydrogenase complex reveals that loop movement controls the rate-limiting step. J Am Chem Soc 134(45):18644-18655. doi:10.1021/ja3062375
2. Blazeck J, Liu L, Redden H, Alper H (2011) Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol 77(22):7905-7914. doi:10.1128/aem.05763-11
3. Bunik VI, Degtyarev D (2008) Structure-function relationships in the 2-oxo acid dehydrogenase family: substrate-specific signatures and functional predictions for the 2-oxoglutarate dehydrogenase-like proteins. Proteins 71(2):874-890. doi:10.1002/prot.21766 (Pubitemid 351430022)
4. Byrne KL, Meacock PA (2001) Thiamin auxotrophy in yeast through altered cofactor dependence of the enzyme acetohydroxyacid synthase. Microbiology 147:2389-2398 (Pubitemid 32899235)
5. Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J (2013) Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 15:48-54. doi:10.1016/j.ymben.2012.11.002
6. Chernyavskaya OG, Shishkanova NV, Il'chenko AP, Finogenova TV (2000) Synthesis of α-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol. Appl Microbiol Biotechnol 53(2):152-158. doi:10.1007/s002530050002 (Pubitemid 30120566)
7. Ciszak EM, Korotchkina LG, Dominiak PM, Sidhu S, Patel MS (2003) Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase. J Biol Chem 278(23):21240-21246. doi:10.1074/jbc.M300339200 (Pubitemid 36806439)
8. Fickers P, Benetti PH, Wache Y, Marty A, Mauersberger S, Smit MS, Nicaud JM (2005) Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res 5(6-7):527-543. doi:10.1016/j.femsyr.2004.09.004 (Pubitemid 40385096)
9. Forster A, Jacobs K, Juretzek T, Mauersberger S, Barth G (2007) Overexpression of the ICL1 gene changes the product ratio of citric acid production by Yarrowia lipolytica. Appl Microbiol Biotechnol 77(4):861-869. doi:10.1007/s00253-007-1205-4 (Pubitemid 350159857)
10. Holz M, Förster A, Mauersberger S, Barth G (2009) Aconitase overexpression changes the product ratio of citric acid production by Yarrowia lipolytica. Appl Microbiol Biotechnol 81(6):1087-1096. doi:10.1007/s00253-008- 1725-6
11. Lessard IA, Perham RN (1994) Expression in Escherichia coli of genes encoding the E1 alpha and E1 beta subunits of the pyruvate dehydrogenase complex of Bacillus stearothermophilus and assembly of a functional E1 component in vitro. J Biol Chem 269(14):10378-10383
12. Lessard IAD, Domingo GJ, Borges A, Perham RN (1998) Expression of genes encoding the E2 and E3 components of the Bacillus stearothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interaction in assembly in vitro. Eur J Biochem 258(2):491-501. doi:10.1046/j.1432-1327.1998.2580491.x (Pubitemid 28557915)
13. Liu L, Li Y, Zhu Y, Du G, Chen J (2007) Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level. Metab Eng 9(1):21-29. doi:10.1016/j.ymben.2006.07.007 (Pubitemid 44960869)
14. Madzak C, Gaillardin C, Beckerich JM (2004) Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol 109(1-2):63-81. doi:10.1016/j.jbiotec.2003.10.027 (Pubitemid 38452572)
15. Mansour S, Bailly J, Delettre J, Bonnarme P (2009) A proteomic and transcriptomic view of amino acids catabolism in the yeast Yarrowia lipolytica. Proteomics 9(20):4714-4725. doi:10.1002/pmic.200900161
16. Morgunov IG, Kamzolova SV, Perevoznikova OA, Shishkanova NV, Finogenova TV (2004) Pyruvic acid production by a thiamine auxotroph of Yarrowia lipolytica. Process Biochem 39(11):1469-1474. doi:10.1016/S0032-9592(03)00259-0 (Pubitemid 38871475)
17. Nicaud JM (2012) Yarrowia lipolytica. Yeast 29(10):409-418. doi:10.1002/yea.2921
18. Otto C, Yovkova V, Aurich A, auersberger S, Barth G (2012) Variation of the by-product spectrum during α-ketoglutaric acid production from raw glycerol by overexpression of fumarase and pyruvate carboxylase genes in Yarrowia lipolytica . Appl Microbiol Biotechnol 95(4):905-917. doi:10.1007/s00253-012-4085-1
19. Otto C, Yovkova V, Barth G (2011) Overproduction and secretion of α-ketoglutaric acid by microorganisms. Appl Microbiol Biotechnol 92(4):689-695. doi:10.1007/s00253-011-3597-4
20. Ren LJ, Huang H, Xiao AH, Lian M, Jin LJ, Ji XJ (2009) Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308. Bioprocess Biosyst Eng 32(6):837-843. doi:10.1007/s00449-009-0310-4
21. Russell GC, Guest JR (1990) Overexpression of restructured pyruvate dehydrogenase complexes and site-directed mutagenesis of a potential active-site histidine residue. Biochem J 269(2):443-450 (Pubitemid 20236037)
22. Shiba Y, Paradise EM, Kirby J, Ro D-K, Keasing JD (2007) Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metab Eng 9(2):160-168. doi:10.1016/j.ymben.2006.10. 005 (Pubitemid 46331877)
23. Soo P-C, Horng Y-T, Lai M-J, Wei J-R, Hsieh S-C, Chang Y-L, Tsai Y-H, Lai H-C (2007) Pirin regulates pyruvate catabolism by interacting with the pyruvate dehydrogenase E1 subunit and modulating pyruvate dehydrogenase activity. J Bacteriol 189(1):109-118. doi:10.1128/jb.00710-06 (Pubitemid 46036226)
24. Strumilo S, Czerniecki J, Dobrzyn P (1999) Regulatory effect of thiamin pyrophosphate on pig heart pyruvate dehydrogenase complex. Biochem Biophys Res Commun 256(2):341-345. doi:10.1006/bbrc.1999.0321 (Pubitemid 29298886)
25. Vemuri GN, Eiteman MA, McEwen JE, Olsson L, Nielsen J (2007) Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104(7):2402-2407. doi:10.1073/pnas.0607469104 (Pubitemid 46391409)
26. Wu SX, Letchworth GJ (2004) High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. Biotechniques 36(1):152-154
27. Xu G, Hua Q, Duan N, Liu L, Chen J (2012) Regulation of thiamine synthesis in Saccharomyces cerevisiae for improved pyruvate production. Yeast 29(6):209-217. doi:10.1002/yea.2902
28. Yu ZZ, Du GC, Zhou JW, Chen J (2012) Enhanced α-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06 by an improved integrated fed-batch strategy. Bioresour Technol 114:597-602. doi:10.1016/j.biortech.2012.03.021
29. Yuzbashev TV, Yuzbasheva EY, Sobolevskaya TI, Laptev IA, Vybornaya TV, Larina AS, Matsui K, Fukui K, Sineoky SP (2010) Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica. Biotechnol Bioeng 107(4):673-682. doi:10.1002/bit.22859
30. Zhou JW, Yin XX, Madzak C, Du GC, Chen J (2012) Enhanced α-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism. J Biotechnol 161(3):257-264. doi:10.1016/j.jbiotec. 2012.05.025
31. Zhou JW, Zhou HY, Du GC, Liu LM, Chen J (2010) Screening of a thiamine-auxotrophic yeast for α-ketoglutaric acid overproduction. Lett Appl Microbiol 51(3):264-271. doi:10.1111/j.1472-765X.2010.02889.x