Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308

Bioprocess and Biosystems Engineering

Volumn 32, Issue 6, 2009, Pages 837-843

  Ren, Lu Jing ^a   Huang, He ^a   Xiao, Ai Hua ^a   Lian, Min ^a   Jin, Li Jing ^a   Ji, Xiao Jun ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

Acetyl CoA; DHA; NADPH; Schizochytrium sp.

Indexed keywords

ACETYL-COA; DHA; DOCOSAHEXAENOIC ACID; ENZYMATIC ACTIVITIES; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; MALIC ACIDS; MALIC ENZYMES; NADPH; SCHIZOCHYTRIUM; SCHIZOCHYTRIUM SP.; TOTAL LIPIDS;

ELECTRIC CONDUCTIVITY MEASUREMENT; ENZYMES; FATTY ACIDS; FERMENTATION; GLUCOSE;

ACIDS;

ACETYL COENZYME A; ADENOSINE TRIPHOSPHATE CITRATE LYASE; DOCOSAHEXAENOIC ACID; FATTY ACID; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; MALATE DEHYDROGENASE (DECARBOXYLATING); MALIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ALGA; ARTICLE; BIOMASS; CONTROLLED STUDY; ENZYME ACTIVITY; FATTY ACID SYNTHESIS; FERMENTATION; LIPID STORAGE; NONHUMAN; PRIORITY JOURNAL; REGULATORY MECHANISM; SCHIZOCHYTRIUM;

ACETYL COENZYME A; ALGAE; ATP CITRATE (PRO-S)-LYASE; BIOREACTORS; BIOTECHNOLOGY; CULTURE MEDIA; DOCOSAHEXAENOIC ACIDS; EUKARYOTIC CELLS; FATTY ACIDS; FERMENTATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; KINETICS; MALATE DEHYDROGENASE; NADP;

SCHIZOCHYTRIUM;

EID: 69249221546     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-009-0310-4     Document Type: Article

References (24)

1. C Agostoni E Riva S Trojan R Bellu M Giovannini 1995 Docosahexaenoic acid status and development quotient of healthy term infants Lancet 346 8975 638
2. AH Lloyd KY Young 1999 Health benefits of docohexaenoic acid (DHA) Pharmacol Res 40 3 211 225
3. L Sijtsma ME Swaaf 2004 Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid Appl Microbiol Biotechnol 64 146 153
4. T Yokochi D Honda T Higashihara, et al. 1998 Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 Appl Microbiol Biotechnol 29 72 76
5. Barclay W, Weaver C, Metz J (2005) Development of a docosahexaenoic acid production technology using Schizochytrium: a historical perspective. In: Cohen Z, Ratledge C (eds) Single cell oils. AOCS Press, Champaign
6. GW Harrington GG Holz Jr 1968 The monoenoic and docosahexaenoic fatty acids of a heterotrophic dinoflagellate Biochim Biophys Acta 164 137 139
7. HB David WH Glenn G Joannel, et al. 1974 Biosynthesis of oleic acid and docosahexaenoic acid by a heterotrophic marine dinoflagellate Crypthcodinium cohnii Biochim Biophys 369 16 24
8. T Yaguchi S Tanaka T Yokochi, et al. 1997 Production of high yields of docosahexaenoic acid by Schizochytrium sp strain SR21 J Am Oil Chem Soc 74 1431 1434
9. E Ganuza MS Izquierdo 2007 Lipid accumulation in Schizochytrium G13/2S produced in continuous culture Appl Microbiol Biotechnol 270 985 990
10. E Ganuza AJ Anderson C Raledge 2008 High-cell-density cultivation of Schizochytrium sp. in an ammonium/pH-auxostat fed-batch system Biotechnol Lett 30 9 1559 1564
11. C Ratledge JP Wynn 2002 The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms Adv Appl Microbiol 51 1 51
12. C Ratledge 2004 Fatty acid biosynthesis in microorganisms being used for single cell oil production Biochimie 86 807 815
13. JP Wynn C Ratledge 1997 Malic enzyme is a major source of NADPH for lipid accumulation by Aspergillus nidulans Microbiology 143 53 257
14. TW Randolph 1989 Malic enzyme of higher plants characteristics, regulation, and physiological function Plant Physiol 90 367 371
15. ME de Swaaf TC de Rijk, et al. 1999 Optimisation of docosahexaenoic acid production in batch cultivations by Crypthecodimium cohnii J Biotechnol 70 185 192
16. ME de Swaaf L Sijtsma JT Pronk 2003 High-cell-density fedbatch cultivation of the docosahexaenoic-acid producing marine alga Crypthecodinium cohnii Biotechnol Bioeng 81 666 672 (Pubitemid 36258519)
17. ME de Swaaf JT Pronk L Sijtsma 2003 Fed-batch cultivation of the docosahexaenoic acid producing marine alga Crypthecodinium cohnii on ethanol Appl Microbiol Biotechnol 61 40 43 (Pubitemid 36458051)
18. E Indarti MIA Majid R Hashim A Chong 2005 Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil J Food Compost Anal 18 161 170
19. J RodrI guez-Ruiz EH Belarbi JLG Sa nchez, et al. 1998 Rapid simultaneous lipid extraction and transesterification for fatty acid analyses Biotechnol Technol 12 689 691
20. OH Lowry NJ Rosebrough AL Farr RJ Randall 1951 Protein measurement with the Folin phenol reagent J Biol Chem 193 265 275
21. RY Hsu HA Lardy 1969 Malic enzyme Methods Enzymol 13 230 235
22. RC Langdon 1966 Glucose 6-phosphate dehydrogenases from erythrocytes Methods Enzymol 9 126 131
23. Y Takeda F Suzuki H Inoue 1969 ATP citrate lyase (citrate-cleavage enzyme) Methods Enzymol 13 153 160
24. Sijtsma L, Anderson AJ, Ratledge C (2005) Alternative carbon sources for heterotrophic production of docosahexaenoic acid by the marine alga Crythecodinium cohnii. In: Cohen Z, Ratledge C (eds) Single cell oils. AOCS Press, Champaign