Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 32, 2014, Pages 11697-11702

  Rose, Honor M ^a   Witte, Christopher ^a   Rossella, Federica ^a   Klippel, Stefan ^a,b   Freund, Christian ^b   Schröder, Leif ^a  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Spin hyperpolarization; Targeted imaging

Indexed keywords

AVIDIN; BIOTIN; CD14 ANTIGEN; CELL SURFACE MARKER; CONTRAST MEDIUM; FLUORESCEIN; IMMUNOGLOBULIN G2B; MACROGOL; UNCLASSIFIED DRUG; XENON 129;

ARTICLE; BINDING AFFINITY; BIOSENSOR; CELL SURFACE; CELL SUSPENSION; CONTROLLED STUDY; FIBROBLAST CULTURE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HYPERPOLARIZATION; MOLECULAR IMAGING; NUCLEAR MAGNETIC RESONANCE IMAGING; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; XENON NUCLEAR MAGNETIC RESONANCE;

SPIN HYPERPOLARIZATION; TARGETED IMAGING;

ANIMALS; ANTIBODY-PRODUCING CELLS; ANTIGENS, CD14; BIOPHYSICAL PHENOMENA; BIOSENSING TECHNIQUES; CELL LINE; CONTRAST MEDIA; IMAGE PROCESSING, COMPUTER-ASSISTED; MAGNETIC RESONANCE IMAGING; MICE; MOLECULAR IMAGING; NANOTECHNOLOGY; NIH 3T3 CELLS; POLYCYCLIC COMPOUNDS; SIGNAL-TO-NOISE RATIO; XENON ISOTOPES;

EID: 84905969132     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1406797111     Document Type: Article

References (41)

1. Mugler JP, 3rd, et al. (1997) MR imaging and spectroscopy using hyperpolarized 129Xe gas: preliminary human results. Magn Reson Med 37(6):809-815
2. Driehuys B, et al. (2006) Imaging alveolar-capillary gas transfer using hyperpolarized 129Xe MRI. Proc Natl Acad Sci USA 103(48):18278-18283
3. Mugler JP, 3rd, Altes TA (2013) Hyperpolarized 129Xe MRI of the human lung. J Magn Reson Imaging 37(2):313-331
4. Nikolaou P, et al. (2013) Near-unity nuclear polarization with an open-source 129Xe hyperpolarizer for NMR and MRI. Proc Natl Acad Sci USA 110(35):14150-14155
5. Schröder L (2013) Xenon for NMR biosensing-Inert but alert. Phys Med 29(1):3-16
6. Spence MM, et al. (2001) Functionalized xenon as a biosensor. Proc Natl Acad Sci USA 98(19):10654-10657
7. Spence MM, et al. (2004) Development of a functionalized xenon biosensor. J Am Chem Soc 126(46):15287-15294
8. Wei Q, et al. (2006) Designing 129Xe NMR biosensors for matrix metalloproteinase detection. J Am Chem Soc 128(40):13274-13283
9. Seward GK, Wei Q, Dmochowski IJ (2008) Peptide-mediated cellular uptake of cryptophane. Bioconjug Chem 19(11):2129-2135
10. Garimella PD, et al. (2014) Hyperpolarized xenon-based molecular sensors for labelfree detection of analytes. J Am Chem Soc 136(1):164-168
11. Kunth M, Döpfert J, Witte C, Rossella F, Schröder L (2012) Optimized use of reversible binding for fast and selective NMR localization of caged xenon. Angew Chem Int Ed Engl 51(33):8217-8220
12. Schröder L, Lowery TJ, Hilty C, Wemmer DE, Pines A (2006) Molecular imaging using a targeted magnetic resonance hyperpolarized biosensor. Science 314(5798):446-449
13. Witte C, Kunth M, Döpfert J, Rossella F, Schröder L (2012) Hyperpolarized xenon for NMR and MRI applications. J Vis Exp 67:4268
14. Vinogradov E, Sherry AD, Lenkinski RE (2013) CEST: From basic principles to applications, challenges and opportunities. J Magn Reson 229:155-172
15. Terreno E, Castelli DD, Aime S (2010) Encoding the frequency dependence in MRI contrast media: The emerging class of CEST agents. Contrast Media Mol Imaging 5(2): 78-98
16. Bai Y, Hill PA, Dmochowski IJ (2012) Utilizing a water-soluble cryptophane with fast xenon exchange rates for picomolar sensitivity NMR measurements. Anal Chem 84(22):9935-9941
17. Stevens TK, Ramirez RM, Pines A (2013) Nanoemulsion contrast agents with subpicomolar sensitivity for xenon NMR. J Am Chem Soc 135(26):9576-9579
18. Huber G, et al. (2006) Water soluble cryptophanes showing unprecedented affinity for xenon: candidates as NMR-based biosensors. J Am Chem Soc 128(18):6239-6246
19. Delacour L, et al. (2013) "Clickable" hydrosoluble PEGylated cryptophane as a universal platform for 129Xe magnetic resonance imaging biosensors. Chemistry 19(19): 6089-6093
20. Meldrum T, et al. (2010) A xenon-based molecular sensor assembled on an MS2 viral capsid scaffold. J Am Chem Soc 132(17):5936-5937
21. Boutin C, et al. (2011) Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: The transferrin case. Bioorg Med Chem 19(13):4135-4143
22. Sloniec J, et al. (2013) Biomembrane interactions of functionalized cryptophane-A: Combined fluorescence and 129Xe NMR studies of a bimodal contrast agent. Chemistry 19(9):3110-3118
23. Palaniappan KK, et al. (2013) Molecular imaging of cancer cells using a bacteriophagebased 129Xe NMR biosensor. Angew Chem Int Ed Engl 52(18):4849-4853
24. Klippel S, et al. (2014) Cell tracking with caged xenon: Using cryptophanes as MRI reporters upon cellular internalization. Angew Chem Int Ed Engl 53(2):493-496
25. Wright SD (1995) CD14 and innate recognition of bacteria. J Immunol 155(1):6-8
26. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249(4975): 1431-1433
27. Schütt C, Schilling T, Krüger C (1991) sCD14 prevents endotoxin inducible oxidative burst response of human monocytes. Allerg Immunol (Leipz) 37(3-4):159-164
28. Te Boekhorst BC, van Tilborg GA, Strijkers GJ, Nicolay K (2012) Molecular MRI of inflammation in atherosclerosis. Curr Cardiovasc Imaging Rep 5(1):60-68
29. Daldrup-Link HE, et al. (2011) MRI of tumor-associated macrophages with clinically applicable iron oxide nanoparticles. Clin Cancer Res 17(17):5695-5704
30. Schnurr M, Witte C, Schröder L (2013) Functionalized 129Xe as a potential biosensor for membrane fluidity. Phys Chem Chem Phys 15(34):14178-14181
31. Sano K, et al. (2011) A pre-targeting strategy for MR imaging of functional molecules using dendritic Gd-based contrast agents. Mol Imaging Biol 13(6):1196-1203
32. Sakahara H, Saga T (1999) Avidin-biotin system for delivery of diagnostic agents. Adv Drug Deliv Rev 37(1-3):89-101
33. Sung C, van Osdol WW (1995) Pharmacokinetic comparison of direct antibody targeting with pretargeting protocols based on streptavidin-biotin binding. J Nucl Med 36(5):867-876
34. Frost SH, Jensen H, Lindegren S (2010) In vitro evaluation of avidin antibody pretargeting using 211At-labeled and biotinylated poly-L-lysine as effector molecule. Cancer 116(4, Suppl):1101-1110
35. Foulon CF, Alston KL, Zalutsky MR (1998) Astatine-211-labeled biotin conjugates resistant to biotinidase for use in pretargeted radioimmunotherapy. Nucl Med Biol 25(2):81-88
36. Hamblett KJ, et al. (2002) A streptavidin-biotin binding system that minimizes blocking by endogenous biotin. Bioconjug Chem 13(3):588-598
37. Rusckowski M, Fogarasi M, Fritz B, Hnatowich DJ (1997) Effect of endogenous biotin on the applications of streptavidin and biotin in mice. Nucl Med Biol 24(3):263-268
38. Carnaroglio D, et al. (2013) One-pot sequential synthesis of isocyanates and urea derivatives via a microwave-assisted Staudinger-aza-Wittig reaction. Beilstein J Org Chem 9:2378-2386
39. Witte C, Kunth M, Rossella F, Schröder L (2014) Observing and preventing rubidium runaway in a direct-infusion xenon-spin hyperpolarizer optimized for high-resolution hyper-CEST (chemical exchange saturation transfer using hyperpolarized nuclei) NMR. J Chem Phys 140(8):084203
40. Zaiss M, Schnurr M, Bachert P (2012) Analytical solution for the depolarization of hyperpolarized nuclei by chemical exchange saturation transfer between free and encapsulated xenon (HyperCEST). J Chem Phys 136(14):144106
41. Hennig J, Nauerth A, Friedburg H (1986) RARE imaging: A fast imaging method for clinical MR. Magn Reson Med 3(6):823-833.