Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

Protein Expression and Purification

Volumn 101, Issue , 2014, Pages 157-164

  Vinther, Lena ^a   Lademann, Ulrik ^a   Andersen, Elisabeth Veyhe ^a   Højrup, Peter ^b   Thaysen Andersen, Morten ^b   Krogh, Berit Olsen ^c   Viuff, Birgitte ^a   Brünner, Nils ^a   Stenvang, Jan ^a   Moreira, José M A ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^c NOVO NORDISK A S   (Denmark)

Author keywords

Bioactive; HEK293E expression system; His tagged; Tissue inhibitor of metalloproteinase 1 protein

Indexed keywords

GELATINASE A; MATRIX METALLOPROTEINASE INHIBITOR; MMP2 PROTEIN, HUMAN; RECOMBINANT PROTEIN; TIMP1 PROTEIN, HUMAN; TISSUE INHIBITOR OF METALLOPROTEINASE 1;

AMINO ACID SEQUENCE; BIOSYNTHESIS; BREAST NEOPLASMS; CELL PROLIFERATION; CELL SURVIVAL; DRUG EFFECTS; FEMALE; GENE EXPRESSION; GENETICS; HEK293 CELL LINE; HUMAN; MASS SPECTROMETRY; MCF 7 CELL LINE; METABOLISM; MOLECULAR GENETICS; TUMOR CELL LINE;

AMINO ACID SEQUENCE; BREAST NEOPLASMS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; FEMALE; GENE EXPRESSION; HEK293 CELLS; HUMANS; MATRIX METALLOPROTEINASE 2; MATRIX METALLOPROTEINASE INHIBITORS; MCF-7 CELLS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TISSUE INHIBITOR OF METALLOPROTEINASE-1;

EID: 84905251910     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.06.013     Document Type: Article

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