Deep Imaging: The next frontier in microscopy

Histochemistry and Cell Biology

Volumn 142, Issue 2, 2014, Pages 125-131

  Roukos, Vassilis ^a   Misteli, Tom ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Deep Imaging; High content screening; High throughput imaging

Indexed keywords

DNA; DNA BINDING PROTEIN; SMALL INTERFERING RNA;

CELL CYCLE; CHROMOSOME TRANSLOCATION; DEEP IMAGING; DNA CONTENT; DNA DAMAGE; DNA STRAND BREAKAGE; HELA CELL LINE; HIGH CONTENT SCREENING; HIGH THROUGHPUT IMAGING; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGING; MICROSCOPE; MICROSCOPY; MOLECULAR BIOLOGY; MOLECULE; OPTICS; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVIEW; RNA INTERFERENCE; GENE TRANSLOCATION; IMAGE PROCESSING; PROCEDURES; TUMOR CELL LINE;

CELL LINE, TUMOR; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSLOCATION, GENETIC;

EID: 84905044640     PISSN: 09486143     EISSN: 1432119X     Source Type: Journal    
DOI: 10.1007/s00418-014-1239-5     Document Type: Review

References (32)

1. Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science (New York, NY) 263:802-805 (Pubitemid 24093206)
2. Conrad C, Gerlich DW (2010) Automated microscopy for high-content RNAi screening. J Cell Biol 188:453-461. doi:10.1083/jcb.200910105
3. Cremer C, Cremer T (1978) Considerations on a laser-scanning-microscope with high resolution and depth of field. Microsc Acta 81:31-44 (Pubitemid 9015453)
4. Fong KW et al (2013) Whole-genome screening identifies proteins localized to distinct nuclear bodies. J Cell Biol 203:149-164. doi: 10.1083/jcb.201303145
5. Hakim O et al (2012) DNA damage defines sites of recurrent chromosomal translocations in B lymphocytes. Nature 484:69-74. doi:10.1038/nature10909
6. Hasson SA et al (2013) High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy. Nature 504:291-295. doi:10.1038/nature12748
7. Heun P, Laroche T, Shimada K, Furrer P, Gasser SM (2001) Chromosome dynamics in the yeast interphase nucleus. Science (New York, NY) 294:2181-2186. doi:10.1126/science.1065366 (Pubitemid 33150678)
8. Krawczyk PM et al (2012) Chromatin mobility is increased at sites of DNA double-strand breaks. J Cell Sci. doi:10.1242/jcs.089847
9. Lazarides E, Weber K (1974) Actin antibody: the specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci USA 71:2268-2272
10. Mao Z, Bozzella M, Seluanov A, Gorbunova V (2008) DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells. Cell Cycle 7:2902-2906
11. Mazzarello P (1999) A unifying concept: the history of cell theory. Nat Cell Biol 1:E13-E15. doi:10.1038/8964
12. Meaburn KJ, Misteli T, Soutoglou E (2007) Spatial genome organization in the formation of chromosomal translocations. Semin Cancer Biol 17:80-90. doi:10.1016/j.semcancer.2006.10.008 (Pubitemid 46054486)
13. Misteli T (2007) Beyond the sequence: cellular organization of genome function. Cell 128:787-800. doi:10.1016/j.cell.2007.01.028
14. Mitelman F, Johansson B, Mertens F (2007) The impact of translocations and gene fusions on cancer causation. Nat Rev Cancer 7:233-245. doi:10.1038/nrc2091 (Pubitemid 46480968)
15. Neumann B et al (2010) Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464:721-727. doi:10.1038/nature08869
16. Nieland TJ et al (2014) High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons. PloS One 9:e91744. doi:10.1371/journal.pone.0091744
17. Patterson G, Davidson M, Manley S, Lippincott-Schwartz J (2010) Superresolution imaging using single-molecule localization. Annu Rev Phys Chem 61:345-367. doi:10.1146/annurev.physchem.012809.103444
18. Pegoraro G, Voss TC, Martin SE, Tuzmen P, Guha R, Misteli T (2012) Identification of mammalian protein quality control factors by high-throughput cellular imaging. PloS One 7:e31684. doi:10.1371/journal.pone.0031684
19. Pepperkok R, Ellenberg J (2006) High-throughput fluorescence microscopy for systems biology. Nat Rev Mol Cell Biol 7:690-696. doi:10.1038/nrm1979 (Pubitemid 44268214)
20. Roix JJ, McQueen PG, Munson PJ, Parada LA, Misteli T (2003) Spatial proximity of translocation-prone gene loci in human lymphomas. Nat Genet 34:287-291. doi:10.1038/ng1177 (Pubitemid 36792860)
21. Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26:1074-1081 (Pubitemid 9076936)
22. Roukos V, Misteli T (2014) The biogenesis of chromosome translocations. Nat Cell Biol 16:293-300. doi:10.1038/ncb2941
23. Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T (2013) Spatial dynamics of chromosome translocations in living cells. Science (New York, NY) 341:660-664. doi:10.1126/science.1237150
24. Roukos V, Burgess RC, Misteli T (2014) Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc (in press)
25. Shen J, Cookson MR (2004) Mitochondria and dopamine: new insights into recessive parkinsonism. Neuron 43:301-304. doi:10.1016/j.neuron.2004.07.012 (Pubitemid 39061122)
26. Simpson JC, Neubrand VE, Wiemann S, Pepperkok R (2001) Illuminating the human genome. Histochem Cell Biol 115:23-29 (Pubitemid 32202050)
27. Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok R (2004) High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Genome Res 14:1948-1956. doi:10.1101/gr.2658304 (Pubitemid 39386509)
28. Symington LS, Gautier J (2011) Double-strand break end resection and repair pathway choice. Annu Rev Genet 45:247-271. doi:10.1146/annurev-genet- 110410-132435
29. Vizeacoumar FJ et al (2010) Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis. J Cell Biol 188:69-81. doi:10.1083/jcb.200909013
30. Walter J, Schermelleh L, Cremer M, Tashiro S, Cremer T (2003) Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. J Cell Biol 160:685-697. doi:10.1083/jcb.200211103 (Pubitemid 36298264)
31. Weinstock DM, Richardson CA, Elliott B, Jasin M (2006) Modeling oncogenic translocations: distinct roles for double-strand break repair pathways in translocation formation in mammalian cells. DNA Repair 5:1065-1074. doi:10.1016/j.dnarep.2006.05.028 (Pubitemid 44291619)
32. Zhang Y et al (2012) Spatial organization of the mouse genome and its role in recurrent chromosomal translocations. Cell 148:908-921. doi:10.1016/j.cell.2012.02.002