Validation of the only commercially available immunoassay for synthetic cathinones in urine: Randox Drugs of Abuse V Biochip Array Technology

Drug Testing and Analysis

Volumn 6, Issue 7-8, 2014, Pages 728-738

  Ellefsen, Kayla N ^a,b   Anizan, Sébastien ^a   Castaneto, Marisol S ^a,b   Desrosiers, Nathalie A ^a,b   Martin, Thomas M ^c   Klette, Kevin L ^c   Huestis, Marilyn A ^a  

^a NATIONAL INSTITUTE ON DRUG ABUSE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c Office of the Secretary of Defense   (United States)

Author keywords

Immunoassay; MDPV; Mephedrone; Randox; Synthetic cathinones

Indexed keywords

BIOCHIPS; BODY FLUIDS; CONTROLLED DRUG DELIVERY; DRUG PRODUCTS; IMMUNOLOGY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

BIOCHIP ARRAYS; CATHINONE; DRUG OF ABUSE; HIGH-THROUGHPUT; IMMUNOASSAY; MEPHEDRONE; METHYLENEDIOXYPYROVALERONE; PERFORMANCE; RANDOX; SYNTHETIC CATHINONE;

ANTIBODIES;

4' METHYLMETHCATHINONE; CATHINONE; DESIGNER DRUG; ALKALOID; METHAMPHETAMINE; STREET DRUG;

ANALYZER; ARTICLE; BIOCHIP; CALIBRATION; DRUG ABUSE; DRUG URINE LEVEL; FALSE POSITIVE RESULT; HIGH THROUGHPUT SCREENING; HUMAN; IMMUNOASSAY; LIQUID CHROMATOGRAPHY; MEDICAL TECHNOLOGY; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; URINE PH; VALIDATION PROCESS; ANALOGS AND DERIVATIVES; LIMIT OF DETECTION; PROCEDURES; PROTEIN MICROARRAY; SUBSTANCE ABUSE; URINE; VALIDATION STUDY;

ALKALOIDS; HUMANS; IMMUNOASSAY; LIMIT OF DETECTION; METHAMPHETAMINE; PROTEIN ARRAY ANALYSIS; STREET DRUGS; SUBSTANCE ABUSE DETECTION;

EID: 84904562274     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.1633     Document Type: Article

References (62)

1. Drug Enforcement Administration (DEA). National Forensic Laboratory Information System Special Report: Synthetic Cannabinoids and Synthetic Cathinones Reported in NFLIS, 2009-2010. US DEA, Springfield, Virginia, 2011.
2. F.T. Peters, M.R. Meyer . In vitro approaches to studying the metabolism of new psychoactive compounds. Drug Test. Anal. 2011, 3, 483.
3. European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Annual Report 2012: The State of the Drugs Problem in Europe. EMCDDA, Luxembourg, 2012, pp. 89.
4. M.H. Baumann, J.S. Partilla, K.R. Lehner . Psychoactive 'bath salts': not so soothing. Eur. J. Pharmacol. 2013, 698, 1.
5. J.B. Zawilska, J. Wojcieszak . Designer cathinones--an emerging class of novel recreational drugs. Forensic Sci. Int. 2013, 231, 42.
6. S.L. Hill, S.H. Thomas . Clinical toxicology of newer recreational drugs. Clin. Toxicol. 2011, 49, 705.
7. F. Schifano, A. Albanese, S. Fergus, J.L. Stair, P. Deluca, O. Corazza, Z. Davey, J. Corkery, H. Siemann, N. Scherbaum, M. Farre, M. Torrens, Z. Demetrovics, A.H. Ghodse . Mephedrone (4-methylmethcathinone; 'meow meow'): chemical, pharmacological and clinical issues. Psychopharmacology (Berl) 2011, 214, 593.
8. H.A. Spiller, M.L. Ryan, R.G. Weston, J. Jansen . Clinical experience with and analytical confirmation of 'bath salts' and 'legal highs' (synthetic cathinones) in the United States. Clin. Toxicol. 2011, 49, 499.
9. T.M. Penders, R.E. Gestring, D.A. Vilensky . Intoxication delirium following use of synthetic cathinone derivatives. Am. J. Drug Alcohol Abuse 2012, 38, 616.
10. D. James, R.D. Adams, R. Spears, G. Cooper, D.J. Lupton, J.P. Thompson, S.H. Thomas . Clinical characteristics of mephedrone toxicity reported to the UK National Poisons Information Service. Emerg. Med. J. 2011, 28, 686.
11. K.J. Lusthof, R. Oosting, A. Maes, M. Verschraagen, A. Dijkhuizen, A.G. Sprong . A case of extreme agitation and death after the use of mephedrone in the Netherlands. Forensic Sci. Int. 2011, 206, e93.
12. P.N. Carbone, D.L. Carbone, S.D. Carstairs, S.A. Luzi . Sudden cardiac death associated with methylone use. Am. J. Forensic Med. Pathol. 2013, 34, 26.
13. J.M. Pearson, T.L. Hargraves, L.S. Hair, C.J. Massucci, C.C. Frazee 3rd, U. Garg, B.R. Pietak . Three fatal intoxications due to methylone. J. Anal. Toxicol. 2012, 36, 444.
14. B.L. Murray, C.M. Murphy, M.C. Beuhler . Death following recreational use of designer drug "bath salts" containing 3, 4-Methylenedioxypyrovalerone (MDPV). J. Med. Toxicol. 2012, 8, 69.
15. S. Rojek, M. Klys, M. Strona, M. Maciow, K. Kula . 'Legal highs'-toxicity in the clinical and medico-legal aspect as exemplified by suicide with bk-MBDB administration. Forensic Sci. Int. 2012, 222, e1.
16. I. Vardakou, C. Pistos, C. Spiliopoulou . Drugs for youth via Internet and the example of mephedrone. Toxicol. Lett. 2011, 201, 191.
17. A.R. Winstock, L.R. Mitcheson, P. Deluca, Z. Davey, O. Corazza, F. Schifano . Mephedrone, new kid for the chop? Addiction 2011, 106, 154.
18. European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Risk Assessment Report of a New Pyschoactive Substance- 4-methylmehtcathinone (mephedrone). EMCDDA, Lisbon, 2011.
19. American Association of Poison Control Centers (AAPCC). Alerts: Bath Salts. Available at: http://www.aapcc.org/alerts/bath-salts/ [2 December 2013].
20. Synthetic Drug Abuse Prevention Act of 2012, Congress of the United States, Washington, D.C., 2012, S.3187-139.
21. Drug Enforcement Administration (DEA). Schedules of controlled substances: Temporary placement of three synthetic cathinones into schedule I. Fed. Regist. 2011, 76, 204.
22. National Conference of State Legislatures. Substituted Cathinones (aka 'Bath Salts') Enactments. Available at: http://www.ncsl.org/issues-research/justice/substituted-cathinones-enactments.aspx [2 December 2013].
23. M. Wikstrom, G. Thelander, I. Nystrom, R. Kronstrand . Two fatal intoxications with the new designer drug methedrone (4-methoxymethcathinone). J. Anal. Toxicol. 2010, 34, 594.
24. M. Martin, J.F. Muller, K. Turner, M. Duez, V. Cirimele . Evidence of mephedrone chronic abuse through hair analysis using GC/MS. Forensic Sci. Int. 2012, 218, 44.
25. K.Y. Rust, M.R. Baumgartner, A.M. Dally, T. Kraemer . Prevalence of new psychoactive substances: A retrospective study in hair. Drug Test. Anal. 2012, 4, 402.
26. S.A. Shah, N.I. Deshmukh, J. Barker, A. Petroczi, P. Cross, R. Archer, D.P. Naughton . Quantitative analysis of mephedrone using liquid chromatography tandem mass spectroscopy: Application to human hair. J. Pharm. Biomed. Anal. 2012, 61, 64.
27. H. Torrance, G. Cooper . The detection of mephedrone (4-methylmethcathinone) in 4 fatalities in Scotland. Forensic Sci. Int. 2010, 202, e62.
28. A.J. Dickson, S.P. Vorce, B. Levine, M.R. Past . Multiple-drug toxicity caused by the coadministration of 4-methylmethcathinone (mephedrone) and heroin. J. Anal. Toxicol. 2010, 34, 162.
29. P. Adamowicz, B. Tokarczyk, R. Stanaszek, M. Slopianka . Fatal mephedrone intoxication--a case report. J. Anal. Toxicol. 2013, 37, 37.
30. J.F. Wyman, E.S. Lavins, D. Engelhart, E.J. Armstrong, K.D. Snell, P.D. Boggs, S.M. Taylor, R.N. Norris, F.P. Miller . Postmortem tissue distribution of MDPV following lethal intoxication by 'bath salts'. J. Anal. Toxicol. 2013, 37, 182.
31. B.M. Cawrse, B. Levine, R.A. Jufer, D.R. Fowler, S.P. Vorce, A.J. Dickson, J.M. Holler . Distribution of methylone in four postmortem cases. J. Anal. Toxicol. 2012, 36, 434.
32. A.J. Pedersen, L.A. Reitzel, S.S. Johansen, K. Linnet . In vitro metabolism studies on mephedrone and analysis of forensic cases. Drug Test. Anal. 2013, 5, 430.
33. P. Kriikku, L. Wilhelm, O. Schwarz, J. Rintatalo . New designer drug of abuse: 3, 4-Methylenedioxypyrovalerone (MDPV). Findings from apprehended drivers in Finland. Forensic Sci. Int. 2011, 210, 195.
34. P.D. Maskell, G. De Paoli, C. Seneviratne, D.J. Pounder . Mephedrone (4-methylmethcathinone)-related deaths. J. Anal. Toxicol. 2011, 35, 188.
35. L.K. Sorensen . Determination of cathinones and related ephedrines in forensic whole-blood samples by liquid-chromatography-electrospray tandem mass spectrometry. J. Chromatogr. B 2011, 879, 727.
36. J. Ammann, J.M. McLaren, D. Gerostamoulos, J. Beyer . Detection and quantification of new designer drugs in human blood: Part 1 - Synthetic Cannabinoids. J. Anal.Toxicol. 2012, 36, 372.
37. A. Derungs, S. Schietzel, M.R. Meyer, H.H. Maurer, S. Krahenbuhl, M.E. Liechti . Sympathomimetic toxicity in a case of analytically confirmed recreational use of naphyrone (naphthylpyrovalerone). Clin. Toxicol. 2011, 49, 691.
38. D.M. Wood, S. Davies, M. Puchnarewicz, J. Button, R. Archer, H. Ovaska, J. Ramsey, T. Lee, D.W. Holt, P.I. Dargan . Recreational use of mephedrone (4-methylmethcathinone, 4-MMC) with associated sympathomimetic toxicity. J. Med. Toxicol. 2010, 6, 327.
39. S.L. Thornton, R.R. Gerona, C.A. Tomaszewski . Psychosis from a bath salt product containing flephedrone and MDPV with serum, urine, and product quantification. J. Med. Toxicol. 2012, 8, 310.
40. M. Concheiro, S. Anizan, K. Ellefsen, M.A. Huestis . Simultaneous quantification of 28 synthetic cathinones and metabolites in urine by liquid chromatography-high resolution mass spectrometry. Anal. Bioanal. Chem. 2013, 405, 9437.
41. L.J. Marinetti, H.M. Antonides . Analysis of synthetic cathinones commonly found in bath salts in human performance and postmortem toxicology: Method development, drug distribution and interpretation of results. J. Anal. Toxicol. 2013, 37, 135.
42. H.A. Borek, C.P. Holstege . Hyperthermia and multiorgan failure after abuse of 'bath salts' containing 3, 4-methylenedioxypyrovalerone. Ann. Emerg. Med. 2012, 60, 103.
43. I.A. Ojanpera, P.K. Heikman, I.J. Rasanen . Urine analysis of 3, 4-methylenedioxypyrovalerone in opioid-dependent patients by gas chromatography-mass spectrometry. Ther. Drug Monit. 2011, 33, 257.
44. M. Meyer . Detection of beta-keto amphetamines in Urine within the systematic toxicological analysis (STA) using GC-MS. TIAFT Bull. 2010, 40, 36.
45. K. Zaitsu, M. Katagi, H.T. Kamata, T. Kamata, N. Shima, A. Miki, H. Tsuchihashi, Y. Mori . Determination of the metabolites of the new designer drugs bk-MBDB and bk-MDEA in human urine. Forensic Sci. Int. 2009, 188, 131.
46. M.R. Meyer, J. Wilhelm, F.T. Peters, H.H. Maurer . Beta-keto amphetamines: Studies on the metabolism of the designer drug mephedrone and toxicological detection of mephedrone, butylone, and methylone in urine using gas chromatography-mass spectrometry. Anal. Bioanal. Chem. 2010, 397, 1225.
47. M. Katagi . Metabolism and forensic toxicological analyses of the extensively abused designer drug methylone. TIAFT Bull. 2010, 40, 30.
48. H.T. Kamata, N. Shima, K. Zaitsu, T. Kamata, A. Miki, M. Nishikawa, M. Katagi, H. Tsuchihashi . Metabolism of the recently encountered designer drug, methylone, in humans and rats. Xenobiotica 2006, 36, 709.
49. S. Strano-Rossi, A.B. Cadwallader, X. de la Torre, F. Botre . Toxicological determination and in vitro metabolism of the designer drug methylenedioxypyrovalerone (MDPV) by gas chromatography/mass spectrometry and liquid chromatography/quadrupole time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 2010, 24, 2706.
50. M.R. Meyer, P. Du, F. Schuster, H.H. Maurer . Studies on the metabolism of the α-pyrrolidinophenone designer drug methylenedioxy-pyrovalerone (MDPV) in rat and human urine and human liver microsomes using GC-MS and LC-high-resolution MS and its detectability in urine by GC-MS. J. Mass Spectrom. 2010, 45, 1426.
51. D. Grueninger, R. Englert . Determination of the amphetamine-like designer drugs methcathinone and 4-methylmethcathinone in urine by LC-MS/MS. Ann. Toxicol. Anal. 2011, 23, 7.
52. C. Bell, C. George, A.T. Kicman, A. Traynor . Development of a rapid LC-MS/MS method for direct urinalysis of designer drugs. Drug Test. Anal. 2011, 3, 496.
53. D.P. Kasick, C.A. McKnight, E. Klisovic . "Bath salt" ingestion leading to severe intoxication delirium: Two cases and a brief review of the emergence of mephedrone use. Am. J. Drug Alcohol Abuse 2012, 38, 176.
54. A.M. Macher, T.M. Penders . False-positive phencyclidine immunoassay results caused by 3, 4-methylenedioxypyrovalerone (MDPV). Drug Test. Anal. 2013, 5, 130.
55. M.J. Swortwood, W.L. Hearn, A.P. Decaprio . Cross-reactivity of designer drugs, including cathinone derivatives, in commercial enzyme-linked immunosorbent assays. Drug Test. Anal. 2013, 405, 1383.
56. J.S. Krouwer, R. Rabinowitz . How to improve estimates of imprecision. Clin. Chem. 1984, 30, 290.
57. R.D. Johnson, S.R. Botch-Jones . The stability of four designer drugs: MDPV, mephedrone, BZP and TFMPP in three biological matrices under various storage conditions. J. Anal. Toxicol. 2013, 37, 51.
58. Y.N.A. Soh, S. Elliott . An investigation of the stability of emerging new psychoactive substances. Drug Test. Anal. 2013, DOI: 10.1002/dta.1576
59. K. Tsujikawa, T. Mikuma, K. Kuwayama, H. Miyaguchi, T. Kanamori, Y.T. Iwata, H. Inoue . Degradation pathways of 4-methylmethcathinone in alkaline solution and stability of methcathinone analogs in various pH solutions. Forensic Sci. Int. 2012, 220, 103.
60. K. Zaitsu, M. Katagi, M. Tatsuno, T. Sato, H. Tsuchihashi, K. Suzuki . Recently abused β-keto derivatives of 3, 4-methylenedioxyphenylalkylamines: A review of their metabolisms and toxicological analysis. Forensic Toxicol. 2011, 29, 73.
61. J.M. Stogner, B.L. Miller . Investigating the 'bath salt' panic: The rarity of synthetic cathinone use among students in the United States. Drug Alcohol Rev. 2013, 32, 545.
62. P.M. O'Byrne, P.V. Kavanagh, S.M. McNamara, S.M. Stokes . Screening of stimulants including designer drugs in urine using a liquid chromatography tandem mass spectrometry system. J. Anal. Toxicol. 2013, 37, 64.